Molecular Devices QPix 420, Руководство пользователя

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QPix 420 Colony Picking System User Guide
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To undo all selections, click the Remove User Selections icon to display the Remove User
Selections dialog. Click Yes.
To select the colonies for picking:
1. From the list in the upper-right area, select the barcode or identifier of the receptacle to
view.
2. Refine the colony Selection criteria.
Compactness sets the level of irregularity for picking colonies. The value is a ratio of
the perimeter divided by the area of the colony, so that irregular shaped colonies are
closer to 0 and colonies that are more of a perfect circle are closer to 1. The system
default value is that a colony equal to or less than 0.65 and is not picked.
Axis Ratio measures how oval the colony is. The value is a ratio of the longest diameter
divided by the shortest diameter, so oval shaped colonies are closer to 0 and round
colonies are closer to 1. The system default value is that a colony equal to or less than
0.65 is not picked.
Min Diameter (mm) sets the minimum diameter of colonies for picking. A colony equal
to or smaller than the value in this field is not picked.
Note: The Min Diameter (mm) cannot be lower than the Diameter value set in the
Debris Discard section of the test image.
Max Diameter (mm) sets the maximum diameter of colonies for picking. A colony equal
to or greater than the value in this field is not picked.
Min Proximity (mm) sets the distance between colonies to pick, so that when picking
one colony, a different adjacent colony is not picked. The system default value is
0.45 mm.