Molecular Devices QPix 420, Руководство пользователя

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Chapter 10: Blue-White Regional Picking Processes
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To manually select blue or white colonies:
To manually select Blue Colonies, select an Intensity Range that starts near the left side
of the histogram and ends to the left of the large spike that represents the largest area,
which is the agar. See Step 6 for Intensity Range setting procedures.
To manually select White Colonies, increase the minimum detectable threshold by
moving the histogram red slider line to the right. This allows the exclusion of the agar,
which is predominantly the large black area in the background, and the colonies that
are less intense than the threshold value.
5. To use Auto Select, click either Blue or White. Click the button as many times as required to
intensify the target colonies. Generally, two or three clicks get you the optimal value. Click
Reset to restore the previous intensity settings.
6. Select the Display tab.
7. Select the method to view the detected colonies in the image.
Click Image Only to display the detected colonies in white with yellow rings and a gray
background.
Click Image and Overlay to display the detected colonies in green with yellow rings
and a gray background.
Click Overlay only to display the detected colonies in white with yellow rings and a
black background.
To remove the yellow ring from the detected colonies, clear the Outline Detected Features
check box.
8. Click the Debris tab.
9. Adjust the Diameter and Axis Ratio to exclude objects that are smaller than the desired
colonies. Each detected feature displays within a yellow ring while excluded features do not
have a yellow ring.
In the Diameter (mm) field, enter a value in millimeters for the minimum diameter of the
required colonies.
In the Axis Ratio field, define the minimum roundness ratio of the required colonies.
The Features Found field displays the number of objects detected as colonies. The value
changes as you make adjustments.
Note: Further refinement for colony detection can be done on a higher-resolution
image in the Feature Selection window. See
Selecting Blue-White Colonies for
Regional Picking on page 132 .
10. Click Next to go to the next step.