MJ Research DNA Engine Dyad PTC-220, Руководство по эксплуатации

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DNA Engine Dyad Operations Manual
To resume a run, select <Resume> from the bottom of the Status window or select <Com-
mand>, <Pause/Resume>, and either <Resume All> or select the desired block from the
drop-down list.
Skipping a Step
To skip to the next step in a running program, select <Skip> at the bottom of the Status
window. A confirmation screen will ask you to confirm the skip, select <Yes> to skip to the
next step in the protocol displayed in the Status window.
Inaccessible Features
Considerations must also be made for the compatibility of the program with the installed
Alpha units. The Run Program window will display one or more “grayed-out” Alpha units in
the
Select Blocks section if there is an incompatibility with the program in queue, or if an
Alpha unit is currently unavailable due to a protocol that is already running.
Some scenarios which may be the cause of inaccessible features or display changes include:
Block control: A sample temperature will not be displayed.
Probe control: Sample temperature will be replaced with Probe temperature. Additionally,
programs with lid temperature steps will not run with probe control.
Gradient step: Alpha units other than 96-well Alpha units will be “grayed-out”.
Lid step: Alpha units will be “grayed-out” if a lid step is included in the protocol, but there
is no Power Bonnet lid installed.
Lid mode: Alpha units will be “grayed-out” if the lid mode is not set to OFF when using a
block with no lid such as a Twin Towers Alpha unit.
When encountering an inaccessible feature or block, please review your program with the
installed Alpha units to determine if an incompatibility is present.
Running Multiple Programs
One particularly useful feature of the Dyad cycler is the ability to run several programs at
once on different blocks. For example, in a Dyad cycler setup with two 96-well Alpha units,
a gradient for optimizing annealing temperature can be run in one Alpha unit, whereas a
typical experiment without a gradient can be run in the other Alpha unit,
simultaneously.
Before running multiple protocols, considerations should be made as to the compatibility of
the program with the available Alpha units. Please review Chapter 4:
Operation for a more
complete treatment of Alpha units.