MJ Research DNA Engine Dyad PTC-220, Руководство по эксплуатации

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4-9
Operation
Microseal
®
'B' adhesive seals feature an aggressive adhesive, effective from –20
°
C to 110
°
C,
which allows secure sample storage or transport before and after cycling. The clear polyes-
ter backing allows easy inspection of sample wells. Microseal 'B' clear, adhesive seals are
ideal for thermal cycling in all polypropylene and polystyrene microplates.
Microseal ® 'M' rubber sealing mats are an economical means to seal 96-well microplates.
An array of 96 dimples on the mat helps orient it on the microplate and prevents the mat from
sticking to the heated lid. The mats may be cleaned with sodium hypochlorite (bleach) for
reuse, and they are autoclavable.
Adjusting the Hot Bonnet Lid’s Pressure
The pressure exerted by the Hot Bonnet lid must be manually adjusted to fit the sample
vessels being used. Once set, the Hot Bonnet lid can be opened and closed repeatedly
without readjustment as long as neither the tube or microplate type nor the sealing method
is changed. Any change in vessel type or sealing method requires readjustment of the
Hot Bonnet lid.
Follow these steps to adjust the pressure exerted by the inner lid:
1. Make sure the block’s wells are clean. Even tiny amounts of extraneous material can
decrease thermal conduction and interfere with the proper seating of a microplate or
tubes.
2. Open the Hot Bonnet lid. Turn the blue thumbwheel all the way counterclockwise to
completely raise the inner lid.
3. Load either a microplate or at least eight individual tubes into the sample block. The
inner lid pivots around a central point, so it is important to distribute individual tubes
evenly: load at least four tubes in the center of the block and at least one tube in each
of the four corners of the block. If using a sealing film or mat, apply it to the loaded
microplate according to the manufacturer’s directions.
4. Close the Hot Bonnet lid by pressing down on the top lever. Turn the thumbwheel
clockwise to lower the inner lid onto the loaded microplate/tubes. The thumbwheel
turns easily at first since the inner lid has not yet come into contact with anything.
Stop turning the thumbwheel when you feel increased resistance, which indicates
that the inner lid has touched the microplate/tubes.
5. For microplate sealing films or mats that require additional pressure, turn the
thumbwheel clockwise an extra half turn past the point of initial contact to set an
appropriate lid pressure.
Caution: Do not turn the thumbwheel more than three-quarters of a turn. This can
make it hard or impossible to close the lid and puts excessive strain on the
latch holding the lid closed.