Mindray BA-88A, Руководство по эксплуатации

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6-2
This calibration method requires only one calibrator.
Two-point linear calibration
Calibration formula:
b aC R
+ =
.
This calibration method adopts two calibration parameters:
a
and
b
, where,
1 2
1 2
C C
R R
a
−
−
=
,
1
1 2
1 2
1
C
C C
R R
R b
） （
−
−
− =
.
This calibration method requires two calibrators.
C
1
and
C
2
are respectively the
concentrations of calibrator 1 and calibrator 2.
R
1
and
R
2
are respectively the
responses of calibrator 1 and calibrator 2.
Multi-point linear calibration
Calibration formula:
b aC R
+ =
.
This calibration method adopts two calibration parameters:
a
and
b
.
This calibration method requires n (n
≥
3) calibrators.
i
C
is the concentration of
calibrator
i
.
i
R
is the response of calibrator
i
.
a
and
b
can be obtained
through the method of least squares.
n C C
n R C R C
a
n
i
i
n
i
i
n
i
i
n
i
i
n
i
i i
/ ) (
/ ) )( (
2
1 1
2
1 1 1
∑ ∑
∑ ∑ ∑
= =
= = =
−
−
=
6.2 Calculating Nonlinear Calibration
Parameters
Logistic-Log 4P
Calibration formula:
)] ln ( exp[ 1
1
0
C b a
K R R
+ − +
+ =
This calibration method adopts four parameters:
0
R
,
K
,
a
and
b
.
This calibration method requires at least four calibrators. The concentration (or
activity) of calibrator 1 is 0, and the corresponding
R
is equal to
R
0
.This
calibration method is applied to the calibration curve that the response becomes
smaller and smaller with the concentration increase. See Figure 6-1.