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Troubleshooting

Before contacting us, ensure that the solution to your technical issue is not in the following table.

Technical issue

Solution

The display shows Err 1, Err 2, Err 3, or Err 4.

See Error Messages.

The instrument does not start.

Ensure the power cord is connected.
Ensure that the power switch is in the on position: “|”.
Check that the 2 fuses at the back of the instrument are functional (value: T3.15A).

There are air bubbles  

in the diluent.

Ensure that the tubing is correctly inserted in the peristaltic pump and follows the direction  

of the flow indicated on the side of the instrument.

The indicated weight  

cannot remain stable.

Ensure that the instrument has been turned on for 10 minutes.
Ensure that the drip tray is properly positioned.
Ensure that the weighing cell holes are free.
Ensure that the instrument is not subjected to any vibrations.
Ensure that the instrument is horizontal.
Ensure that there is no air flow affecting the weighing.
Switch the instrument off and on to re-initialize it.
After following all of the above suggestions, repeat the calibration procedure.

The motor makes a noise  

but the pump does not start.

Ensure that the tubing is correctly positioned in the pump (see Adjusting the Pump  

and Inserting the Tubing).
Ensure that the tubing is not pinched.
Ensure that the rollers of the pump are not blocked.
Ensure that the pumps are set correctly (see Adjusting the Pump and Inserting the Tubing).
Stretch the tubing on both sides without opening the pump.

The pump perforates the tubing.

Ensure that the tubing has been correctly inserted and centered in the pump  

(see Adjusting the Pump and Inserting the Tubing).
Ensure that the pumps are set correctly (see Adjusting the Pump and Inserting the Tubing).

The pump does not intake  

the liquid.

Ensure that the pumps are correctly set (see Adjusting the Pump and Inserting the Tubing).
Ensure that the tubing is correctly inserted in the peristaltic pump and follows the direction  

of the flow indicated on the side of the instrument.

Error Messages

If operation fails, the device displays an error: Err 1, Err 2, Err 3, or Err 4.

Error message

Technical issue

Solution

Err 1 (DiluCult™ 2  

instrument only)

The robotic arm cannot move automatically.

Ensure that nothing is blocking the movement of the arm  

and repeat the process.

Err 2

For a dilution, the weight of the sample is too  

low (< 0.5 g) or too high (the total weight  

after dilution should not exceed the weight  

capacity of the DiluCult™ instrument).

Replace the sample and start the dilution again.

Err 3

The liquid does not flow.

Ensure that the tubing is correctly placed in the pump and 

nothing is preventing the liquid from flowing in the tubing.  

Press the 

Go

 button again.

Err 4

At the end of a calibration, one of the  

2 measures (the empty weight or the  

500 g calibrated weight) is wrong.

Ensure that nothing is resting on the weighing platform  

that could alter the measurement.

Содержание DiluCult

Страница 1: ...sigmaaldrich com User Guide DiluCult and DiluCult 2 instruments The life science business of Merck operates as MilliporeSigma in the US and Canada...

Страница 2: ...ers 3 Operator and Equipment Safety 3 System Components 5 Setting Up the Instrument 6 Unpacking the Instrument 6 Installing the Instrument 6 Operating the Instrument 11 Powering the Instrument On 11 E...

Страница 3: ...on Do not expose the DiluCult and DiluCult 2 instruments to liquid If liquid gets inside the instrument through the ventilation cut outs at the back of the instrument avoid any contact with the instru...

Страница 4: ...ion time for a 375 g sample diluted at 1 10 80 s Dispensing time for 90 mL 7 s 7 s Dispensing time for 225 mL 10 s 10 s Maximum number of pumps 2 integrated 2 integrated Dispensing distribution mode F...

Страница 5: ...acy sticker RS232 output USB output System Components Tubing holder Removable drip tray Fixed dispensing arm Nozzle holder Bag holder DiluCult Instrument Front Back On Off switch Identification sticke...

Страница 6: ...DiluCult 2 Instrument The shipping box contains the following One dispensing assembly 6 4 mm diameter for GL45 bottle per pump One set of 10 GeckoGrip One set of adhesive gum One monitoring software C...

Страница 7: ...t Instrument DiluCult 2 Instrument 2 1 Nozzle protector Installing the Bag Holder Clip the bag holder into the two holes in the base of the instrument For the DiluCult 2 instrument only also clip on t...

Страница 8: ...pump extension are not aligned They should be staggered in relation to each other 4 Clip the pump extension on the pump 5 Open the 2 covers If the alignment is incorrect separate the pump from the pum...

Страница 9: ...2 Install the tubing The direction of flow is indicated by the arrows on the housing 3 For tubing with an internal diameter smaller than 6 4 mm delivered as standard see Adjusting the Pump and Insert...

Страница 10: ...r is 6 4 mm To change the setting turn the button on each side of the pump to obtain the right setting Tubing is pinched Tubing is not pinched 2 Insert the tubing into the pump 3 Close the pump keepin...

Страница 11: ...dy to function The arm of the DiluCult 2 instrument is initialized Ensuring the Accuracy of the Weighing Platform To ensure greater accuracy of the weighing platform wait 10 minutes before the first u...

Страница 12: ...ocessing Green indicates that the weighing platform is stable Proceed with the process 11 Start the dilution by pressing the Go button The In progress LED lights This indicates that the dilution is ta...

Страница 13: ...Start the dispensing by pressing the Go button The In progress LED lights This indicates that the dispensing is taking place The light under the instrument turns orange and flashes slowly The robotic...

Страница 14: ...on instructions at the back of the unit button again The instrument beeps 3 times 7 Remove the weight The instrument is calibrated and ready for use Using the Monitoring Software For traceability the...

Страница 15: ...ed is a dilution Displayed as a whole number no decimal places the number N when the dilution ratio is 1 N Sample weight Displayed in grams with 2 decimal places Theoretical weight Displayed when the...

Страница 16: ...g holder Drip tray Fixed arm Nozzle holder including roller Protecting plate for nozzle Follow these maintenance practices Autoclave all the dispensing assemblies every day when working in sterile con...

Страница 17: ...ons correctly Cleaning the Nozzle Holder DiluCult 2 Instrument Only 1 Switch the instrument to standby mode by pressing the button 2 Press the Go button twice The arm initializes 3 Unscrew the nozzle...

Страница 18: ...sure that the pumps are set correctly see Adjusting the Pump and Inserting the Tubing Stretch the tubing on both sides without opening the pump The pump perforates the tubing Ensure that the tubing ha...

Страница 19: ...nsing reports Ensure that the DiluCult instrument is connected to the USB cable Disconnect and then reconnect the cable and ensure that the computer makes a sound every time If the computer does not h...

Страница 20: ...demarks of Merck KGaA Darmstadt Germany or its affiliates All other trademarks are the property of their respective owners Detailed information on trademarks is available via publicly accessible resou...

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