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Using the Function Buttons

Function buttons are located at the bottom of the screen:

To save the process data in a file, click the 

 button. 

Navigate to the appropriate location. The format of  

the saved file is CSV (comma-separated values).
To save the file in spreadsheet format in Microsoft

®

 

Excel

®

, OpenOffice

®

 Calc, or LibreOffice

®

 Calc, the 

spreadsheet software must be installed on the 

computer. Click the 

 button. Navigate to the 

appropriate location.

Click the 

 button to delete the process data  

on the screen.
Click the 

 button to exit the software.

Maintenance

Cleaning the Instrument

During operation, clean up any leakage quickly  

and thoroughly. 

Note: Do not use bleach to clean the DiluCult™ 

instrument housing. Bleach could corrode the 

stainless steel.
During cleaning, do not let any liquid drip into  

the instrument. 

Autoclavable items:

• Dispensing assemblies
• Filter
• Bottle

Non-autoclavable items:

• Instrument housing
• Pumps
• Bag holder
• Drip tray
• Fixed arm
• Nozzle holder (including roller)
• Protecting plate for nozzle

Follow these maintenance practices:

• Autoclave all the dispensing assemblies every day 

when working in sterile conditions.

• Replace the silicone tubing whenever the tubing is 

not air-tight or becomes sticky or stiff.

• Every 20 autoclavings, replace the air filter.
• To rinse the tubing using the DiluCult™ instrument:

• Fill a bottle with water or another rinsing fluid.
• Place the tube weight and the tubing in the bottle.
• Pull the nozzle out of the nozzle holder by leaving  

the tubing in the pump and place the tubing into 

the rinsing bottle.

• Select the distribution mode by pressing  

Mode

dilution distribution

.

• Choose 100 g to dispense and press  

the 

Go

 button. The tubing is rinsed.

• Repeat this operation as many times  

as necessary.

Maintaining the Pump Head

The pump head is not autoclavable.
To clean the pump head:
1.  Unplug the instrument.
2.  Remove dust and dirt with a soft cloth.
3.  Wipe all surfaces with a disinfecting wipe.
Once a week, grease the pump head to prevent 

premature wearing. Apply a universal multi-purpose 

grease to the friction areas:

Содержание DiluCult

Страница 1: ...sigmaaldrich com User Guide DiluCult and DiluCult 2 instruments The life science business of Merck operates as MilliporeSigma in the US and Canada...

Страница 2: ...ers 3 Operator and Equipment Safety 3 System Components 5 Setting Up the Instrument 6 Unpacking the Instrument 6 Installing the Instrument 6 Operating the Instrument 11 Powering the Instrument On 11 E...

Страница 3: ...on Do not expose the DiluCult and DiluCult 2 instruments to liquid If liquid gets inside the instrument through the ventilation cut outs at the back of the instrument avoid any contact with the instru...

Страница 4: ...ion time for a 375 g sample diluted at 1 10 80 s Dispensing time for 90 mL 7 s 7 s Dispensing time for 225 mL 10 s 10 s Maximum number of pumps 2 integrated 2 integrated Dispensing distribution mode F...

Страница 5: ...acy sticker RS232 output USB output System Components Tubing holder Removable drip tray Fixed dispensing arm Nozzle holder Bag holder DiluCult Instrument Front Back On Off switch Identification sticke...

Страница 6: ...DiluCult 2 Instrument The shipping box contains the following One dispensing assembly 6 4 mm diameter for GL45 bottle per pump One set of 10 GeckoGrip One set of adhesive gum One monitoring software C...

Страница 7: ...t Instrument DiluCult 2 Instrument 2 1 Nozzle protector Installing the Bag Holder Clip the bag holder into the two holes in the base of the instrument For the DiluCult 2 instrument only also clip on t...

Страница 8: ...pump extension are not aligned They should be staggered in relation to each other 4 Clip the pump extension on the pump 5 Open the 2 covers If the alignment is incorrect separate the pump from the pum...

Страница 9: ...2 Install the tubing The direction of flow is indicated by the arrows on the housing 3 For tubing with an internal diameter smaller than 6 4 mm delivered as standard see Adjusting the Pump and Insert...

Страница 10: ...r is 6 4 mm To change the setting turn the button on each side of the pump to obtain the right setting Tubing is pinched Tubing is not pinched 2 Insert the tubing into the pump 3 Close the pump keepin...

Страница 11: ...dy to function The arm of the DiluCult 2 instrument is initialized Ensuring the Accuracy of the Weighing Platform To ensure greater accuracy of the weighing platform wait 10 minutes before the first u...

Страница 12: ...ocessing Green indicates that the weighing platform is stable Proceed with the process 11 Start the dilution by pressing the Go button The In progress LED lights This indicates that the dilution is ta...

Страница 13: ...Start the dispensing by pressing the Go button The In progress LED lights This indicates that the dispensing is taking place The light under the instrument turns orange and flashes slowly The robotic...

Страница 14: ...on instructions at the back of the unit button again The instrument beeps 3 times 7 Remove the weight The instrument is calibrated and ready for use Using the Monitoring Software For traceability the...

Страница 15: ...ed is a dilution Displayed as a whole number no decimal places the number N when the dilution ratio is 1 N Sample weight Displayed in grams with 2 decimal places Theoretical weight Displayed when the...

Страница 16: ...g holder Drip tray Fixed arm Nozzle holder including roller Protecting plate for nozzle Follow these maintenance practices Autoclave all the dispensing assemblies every day when working in sterile con...

Страница 17: ...ons correctly Cleaning the Nozzle Holder DiluCult 2 Instrument Only 1 Switch the instrument to standby mode by pressing the button 2 Press the Go button twice The arm initializes 3 Unscrew the nozzle...

Страница 18: ...sure that the pumps are set correctly see Adjusting the Pump and Inserting the Tubing Stretch the tubing on both sides without opening the pump The pump perforates the tubing Ensure that the tubing ha...

Страница 19: ...nsing reports Ensure that the DiluCult instrument is connected to the USB cable Disconnect and then reconnect the cable and ensure that the computer makes a sound every time If the computer does not h...

Страница 20: ...demarks of Merck KGaA Darmstadt Germany or its affiliates All other trademarks are the property of their respective owners Detailed information on trademarks is available via publicly accessible resou...

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