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Dilution
1. Place the bag holder on the instrument.
2. Select dilution mode by pressing the
Mode
dilution distribution
button. Two beeps sound, and the
“Dilution” LED lights.
3. Select the pump (right or left) by pressing
the
left
right
Pump
button.
4. Select one of 3 modes by pressing
the
Speed
accurate standard fast
button:
5. • Fast: Fast flow with lower accuracy
• Standard: Standard flow with standard accuracy
• Accurate: Slow flow with optimal accuracy
Note: Choose the Accurate mode to prime the
instrument for the first dilution after opening
the pumps, changing the tubing, or changing the
container. This guarantees an accuracy greater
than 98%.
6. Select the dilution rate by pressing
the
– +
buttons.
The dilution/distribution mode, the selected
pump, the dilution rate, and the chosen speed
remain in the instrument’s memory even after the
instrument is switched off.
7. Place the bag in the bag holder.
8. Press the
Tare
button. A beep sounds.
9. When the "Place sample" LED lights, place the
sample in the bag. The weight of the sample
appears on the screen.
10. The color of the light under the instrument
indicates the stability of the weighing cell:
• Blue indicates that the weighing cell is not
stable. Wait for the instrument to stabilize
before processing.
• Green indicates that the weighing platform
is stable. Proceed with the process.
11. Start the dilution by pressing the
Go
button.
The “In progress” LED lights. This indicates
that the dilution is taking place. The lights
under the instrument turn orange and flash
slowly. The robotic arm of the DiluCult™ 2
instrument moves forward simultaneously.
12. To stop the dilution at any time, press the
STOP
button. A beep sounds.
13. To resume the dilution, press the
Go
button.
This process can be repeated as many times
as necessary during the dilution. During these
interruptions, the diluent, the tubing, the pump
(right/left), and speed can all be changed before
the current dilution finishes.
The process may also be started all over again.
To stop everything, press the
STOP
button a second
time. Three beeps sound.
When the dilution is finished:
• A beep sounds.
• The final weight is displayed.
• An LED indicates the obtained accuracy
of the dilution:
• Green = high
• Orange = medium
• Red = low
• The lights under the instrument flash quickly,
indicating the accuracy with a color code.
• The robotic arm of the DiluCult™ 2 instrument
moves back.
• To run the process again with the same type
of bag, place a bag in the bag holder and
press the
Go
button.
Note: The weight of the bag remains in the
instrument’s memory. As long as all the bags
weigh the same, multiple samples can be prepared
ahead of time and diluted one after the other
without changing settings on the instrument.
