Knauer Azura V 2.1S, Руководство пользователя

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Application Examples 35
AZURA V 2.1S User Manual, V6860, Version 2.2
Backflushing the Column
The backflushing of a column is used, for example, in the following cases:

analysis of heavily retarding substances

optimization of analysis times

flushing of a column
Note: The eluent flushes the column after switchover in opposite direction.
Make sure there is no pressure when switching so that the column is not dam-
aged.
Backflushing the Precolumn
The precolumn is used in chromatography, for example, in the following cases:

preseparation or separation of heavily retarding substances
Functional Principle
From the valve, the column
1
is filled with sample through the channel 2->3 .
The quicker part of the substances is separated through the channels 6->1 and
5->4 and transported to the detector
2
.
Fig. 24 backflushing - transport of the sample onto the column
After switching over the valve, heavily retarding substances are eluted in oppo-
site direction from the column and are transported back to the detector
2
.
Fig. 25 backflushing of the column
1
2 3
4
5 6
1
2
1
2 3
4
5 6
2
1