37
Calibration
4000 MiD® Instructions, V6695
4.
Attach the DFI to the syringe using the input line provided with the
calibration kit.
Caution
Do not mount the DFI to the MiD during this process
Attention
Ne montez pas le DFI á la MiD au cours de ce proces-
sus
5.
Attach the input line to the syringe.
6.
Using the syringe pump, infuse at least 100 µL of the methanol/water
solution through the DFI at a flow rate of 10 µL/min. The solution will
flow through the DFI and will drip from the end of the flow meter
adaptor.
7.
When the desired amount of solvent has been infused stop the syrin-
ge pump.
8.
Remove the flow meter adaptor from the DFI using the same proce-
dure as when removing a spraychip.
9.
The DFI is now clean and ready for use.
In the event the DFI appears blocked and/or the measured flow rate is in-
correct, then replace the low flow tubing located inside the DFI (see „8.6
Spraychip assembly maintenance“ on page 38).
9.5.2 Cleaning the Split Flow Interface
The following procedure should be used to clean the SFI.
1.
Fill a syringe with a 50:50 (v/v) mixture of HPLC grade methanol and
water with no additives and mount in a syringe pump.
2.
If fitted, remove the SFI from the MiD (see „5.8.3 Installing spraychip
assemblies“ on page 25) and remove the spraychip (see „8.2 Removv-
ing and replacing the spraychip“ on page 33).
3.
Fit the following components of the flow meter assembly to the SFI;
flow meter adapter and adaptor union.
4.
Attach the SFI to the syringe using the adaptor union and the
provided infusion line and fitting (Fig. 36).
Fig. 22
SFI and flow meter adapter fittings
5.
Using the syringe pump flush the SFI.
6.
Infuse at least 200 µL of the methanol/water solution through the SFI.
The solution will drip from the end of the waste and input lines of the
SFI.
Procedure
adapter ferrule
nut
adapter union
infusion line
flow meter adapter
