Knauer 4000 MiD, Инструкция по эксплуатации

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35 Calibration
4000 MiD® Instructions, V6695
Note:
If an increased delay is observed on the split flow interface (SFI)
when using the cleaned spraychip then attempt to clean again. In the
event that this delay continues to be unacceptable then the spraychip
must be replaced.
If the spraychip looks visually degraded or you have any queries
please contact KNAUER before using the device.
9.4 Liquid flow rate measurement
In the case that sensitivity or spray stability remains poor after cleaning
or replacement of the spraychip 200. It is recommended the liquid flow
rate through the spraychip assembly is measured. This can be performed
on both the DFI and SFI. To measure the liquid flow rate, the flow meter
assembly (Fig. 33) will be required.
Fig. 19
Flow meter assembly
1. Remove the spraychip from the DFI/SFI (see „8.2 Removing and re -
placing the spraychip“ on page 33).
2. Fit the flow meter assembly to the DFI or SFI as required, using the
same procedure as when fitting a spraychip. An SFI complete with
the flow meter assembly is shown in Fig. 34.
Fig. 20 SFI with flow meter assembly
3. Set the desired input flow rate for the liquid/solvent supply e.g. LC or
syringe pump.
4. Turn on the pump.
Note: Flow rates should only be measured with the flow me-
ter tube in a horizontal position.
adapter union
flow meter tube
1
/
16
” sleeving
1
/
16
” finger tight nut
flow meter
adapter
Procedure
1.
Fill a syringe with a 50:50 (v/v) mixture of HPLC grade methanol and
water with no additives and mount in a syringe pump.
2. Disconnect the PEEK tubing from the SFI (input and waste line).
3. Install the SFI plug into the waste line port and complete all fluidic
connections between the SFI and the syringe.
4.
Screw the spraychip to be cleaned into the SFI and place it on a lint
free tissue on a flat surface.
Caution Do not mount the SFI to the MiD during this process
Attention Ne montez pas le SFI á la MiD au cours de ce proces-
sus
5. Infuse at least 150 µL of the water/methanol using a flow rate of 10
µL/min. The solution will flood the spraychip during this process.
6. Unscrew the spraychip from the SFI and gently blow dry with com-
pressed air/nitrogen to remove any excess liquid.
7. Leave the spraychip for a few hours to dry completely
8. The spraychip is now clean and ready for use.
Procedure