42
Calibration
4000 MiD® Instructions, V6695
9.
Unscrew the fingertight nuts from the tee and reducing union and
remove the low flow tube.
10.
To fit a new low flow tube, first slide the tube through one of the
fingertight nuts.
11.
Fit the nut to the splitting tee; gently push the tube into the tee whilst
tightening the nut.
12.
Slide the opposite end of the low flow tube into the second finger-
tight nut.
13.
Using the weight of the reducing union to maintain pressure on the
tube, tighten the second fingertight nut into the splitting tee.
14.
Press the tee, union and tube back into position within the SFI.
Ensure the electrical connection for the tee is aligned with the retai-
ning screw aperture. The fittings will click into the metal clip and the
moulding of the SFI base. Ensure the unions are fitted as shown in
Fig. 57.
Note:
The tee and union will only fit into the SFI base in one
configuration. Do not force the unions to fit.
15.
Using the PH1 Phillips screwdriver fit the retaining screw to the split-
16.
Refit the SFI cover and tighten the 3 securing screws using the PH1
screwdriver.
17.
Refit the input line nut and tubing (Fig. 40 and Fig. 41).
18.
Refit the metal collar and PEEK screw by reversing steps 3-6.
The SFI low flow tube has been successfully changed. Fit either a spray-
chip 200 or measure the flow rate (
Liquid flow rate measurement
). If the initi-
al problem persists and/or you have any queries please contact KNAUER.
9.7 Removing and replacing the vac-chip
Please follow the procedure below in the event that the vac-chip has to
be replaced or removed. Please refer to Fig. 44 when following this
procedure.
Caution
Turn off any fume hoods or ventilation directly surround-
ing the MiD when replacing the vac-chip, if safe to do so.
Attention
Lors du remplacement du vac-chip, éteignez les hottes
de ventilation entourant directement le MiD, si le niveau de sécu-
rité le permet.
1.
If the MiD is under vacuum, make sure that the instrument is not
scanning. Turn off any sample/mobile phase pumps to ensure that
fluid is not flowing to the spraychip.
2.
Place the MiD into standby mode, if necessary, by clicking the Ope-
rate/Standby button.
3.
To remove the vac-chip assembly from the MiD first vent the system.
4.
Open the door until at 90˚.
5.
Remove the DFI/SFI from the MiD (see „5.8.3 Installing spraychip
assemblies“ on page 25 for details).
6.
Use the 4mm “T” Allen key loosen the four M4 screws securing
the vac-chip assembly in an alternating criss-cross pattern until the
screws are loose.
Procedure
