4 Calibration
18
4.4
Calibration of UV/VIS
There are three kinds of calibration method for UV/VIS: common zero calibration, common
span calibration, and individual span calibration.
Reference
"4.1 Calibration Pattern and its Cycle" (page 15).
4.4.1
How to Prepare Calibration Solution
Use distilled water for the zero calibration solution, and exclusive calibration solution for the
common span calibration solution.
The calibration solution ampoule (H calibration solution (blue label)) for 1 Abs (100 m
-1
) is
attached to this unit. An attached calibration solution ampoule covers the measurement range
up to 1 Abs. Full scale can be covered from 0.1 Abs to 5.0 Abs (10 m
-1
to 500 m
-1
) by using
the 2 or 4 calibration solution ampoules.
Select the measurement full scale you use from the following, and compound the calibration
solution.
Also, our calibration solution ampoule (H II calibration solution (green label)) for 2 Abs
(200 m
-1
) is also available. If using in the measurement full scale of 1.1 Abs (110 m
-1
) or over,
it is more economical to buy H II calibration solution ampoules as the number of ampoules
used is fewer.
Common zero calibration solution
Preparation
Make sure to use the distilled water.
How to prepare common span calibration solution
Preparation
Calibration solution
Measurement full
scale range
Qty. of calibration
solution ampoule
Concentration of calibration
solution when diluted with 2 L
H calibration
solution(blue label)
0 to 1 Abs
for
calibration
0.1 Abs to 1.0 Abs
(10 m
-1
to 100 m
-1
)
1
UV: 0.690 Abs (69.0 m
-1
)
VIS: 0.880 Abs (88.0 m
-1
)
1.1 Abs to 2.5 Abs
(110 m
-1
to 250 m
-1
)
2
UV: 1.380 Abs (138.0 m
-1
)
VIS: 1.760 Abs (176.0 m
-1
)
2.6 Abs to 5.0 Abs
(260 m
-1
to 500 m
-1
)
4
UV: 2.760 Abs (276.0 m
-1
)
VIS: 3.520 Abs (352.0 m
-1
)
Calibration solution
Measurement full
scale range
Qty. of calibration
solution ampoule
Concentration of calibration
solution when diluted with 2 L
H II calibration solution
(green label)
0 to 2 Abs
for calibration
1.1 Abs to 2.5 Abs
(110 m
-1
to 250 m
-1
)
1
UV: 1.380 Abs (138.0 m
-1
)
VIS: 1.760 Abs (176.0 m
-1
)
2.6 Abs to 5.0 Abs
(260 m
-1
to 500 m
-1
)
2
UV: 2.760 Abs (276.0 m
-1
)
VIS: 3.520 Abs (352.0 m
-1
)
Distilled water:
2 L
Calibration solution ampoule:
1 to 4
Zero solution (distilled water):
approximately 3 L
2 L Measuring flask:
1
Cleaning bottle:
1
