Compass
™
Stainer Operator’s Manual page 45
Staining Protocol, 95% Reagent Alcohol Fixed ThinPrep
®
Slides
for use with ThinPrep Imaging
Equipment: Hologic Compass Stainer Agitation: Dips = 10, Height = 30 mm Exit speed = Normal
Run no more than four slide racks together. Wait for all four to be unloaded before adding new racks.
Caution:
Change the Distilled / Instrument Feed Water bath at
Station 5
after every
four
racks of slides.
Maintain bath heights to completely cover the slides at full immersion.
Note:
Bath fill volume = 360 ml.
Station
Time
Duration
Solution
LOAD
n/a
n/a
95% Reagent or Ethyl alcohol
1
01’00”
Non-critical
70% Reagent or Ethyl alcohol
2
01’00”
Non-critical
50% Reagent or Ethyl alcohol
3
01’00”
Non-critical
Distilled or Instrument Feed Water (IFW)
a
a. Hologic specification for IFW: ≥1.0 megohm-cm Resistivity OR ≤1.0 μSiemens/cm Conductivity (Refer to Clinical and Laboratory Standards
Institute (CLSI) document C3-A4, 2006)
4
07’00”
Critical
ThinPrep Nuclear Stain
5
00’10”
Critical
Distilled or Instrument Feed Water (IFW)
a
(Replace after every 4
racks.)
6
01’00”
Critical
ThinPrep Rinse Solution
7
00’30”
Non-critical
Distilled or Instrument Feed Water (IFW)
a
8
00’30”
Critical
ThinPrep Bluing Solution
9
00’30”
Non-critical
Distilled or Instrument Feed Water (IFW)
a
10
00’30”
Non-critical
50% Reagent or Ethyl alcohol
11
00’30”
Non-critical
95% Reagent or Ethyl alcohol
12
02’00”
Critical
ThinPrep Orange G Solution
13
00’15”
Critical
95% Reagent or Ethyl alcohol
14
00’15”
Critical
95% Reagent or Ethyl alcohol
15
04’00”
Critical
ThinPrep EA Solution
16
01’00”
Critical
95% Reagent or Ethyl alcohol
17
01’00”
Critical
95% Reagent or Ethyl alcohol
18
00’30”
Non-critical
100% Reagent or Ethyl alcohol
19
00’30”
Non-critical
100% Reagent or Ethyl alcohol
20
00’30”
Non-critical
100% Reagent or Ethyl alcohol
21
01’00”
Non-critical
Xylene or other Hologic approved clearing agent
b
b. See ThinPrep Stain User’s Manual, Section 1, “STAINING” or contact Hologic for current list of Hologic approved clearing agents and mounting
medias
22
03’00”
Non-critical
Xylene or other Hologic approved clearing agent
b
UNLOAD
n/a
n/a
Xylene or other Hologic approved clearing agent
b
