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Choose method and tray position

Adjust the focus

Set exposure sensitivity

Expose

Save the image

4.3.2 Choose method and tray position

Action

Step

Click the Method/Tray position button.

Result: The Method/Tray position dialog opens.

Getting Started with ImageQuant LAS 4000 28-9607-59 AB

4 Operation

4.3 Performing an exposure

4.3.1 Workflow

Содержание ImageQuant LAS 4000

Страница 1: ...GE Healthcare Getting Started with ImageQuant LAS 4000 Original instructions...

Страница 2: ......

Страница 3: ...a head to the computer 35 3 5 3 Installing the lens 37 3 5 4 Attaching a filter to the filter changer 39 3 5 5 Installing a transmitted light source 40 3 5 6 Installing Epi light sources 42 4 Operatio...

Страница 4: ...odors are perceived 67 6 3 Problems with ImageQuant LAS 4000 68 6 4 Problems with ImageQuant LAS 4000 Control Software 69 6 5 Message ImageQuant LAS 4000 hardware error occurred 71 6 6 Error messages...

Страница 5: ...be acquainted with the use of general laboratory equipment and with handling of biological materials In this chapter This chapter contains important user information a general description of the Imag...

Страница 6: ...ates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Notices NOTICE NOTICE...

Страница 7: ...the Declaration of Conformity is available on request Title Directive Machinery Directive MD 2006 42 EC Low Voltage Directive LVD 2006 95 EC ElectroMagnetic Compatibility EMC Directive 2004 108 EC Int...

Страница 8: ...1 or relevant harmonized standards Within EU connected equipment must be CE marked 1 3 The ImageQuant LAS 4000 Feature overview The following features are offered by the ImageQuant LAS 4000 Digitized...

Страница 9: ...uant LAS 4000 5 3 6 4 7 1 2 8 IDX interior Intelligent dark box IDX Description Name Part CCD cooling and image data output Camera head 1 Dark box Intelligent dark box IDX 2 F0 85 43 mm LAS High Sens...

Страница 10: ...ht table UV trans tray for UV transilluminator NP tray for titer plates Turn the ImageQuant LAS 4000 on or off Power switch 8 Computer The ImageQuant LAS 4000 is operated using a PC running Windows XP...

Страница 11: ...escent exposure used for overlaying a marker image Add Digitization Image 5 The state of the CCD and exposure type Setting state CCD 6 Current settings of tray position and illumination inside the dar...

Страница 12: ...d or if the equipment becomes unusually hot This may result in fire or electric shock Stop using immediately turn off the power switch and unplug the equipment from the power outlet Contact your local...

Страница 13: ...lass such as lenses filters or lights Personal protection WARNING Parts of this equipment emit UV radiation Avoid exposure Wear protective clothing and eyewear UV radiation can cause severe burns and...

Страница 14: ...ads on one electrical outlet could result in fire and electric shock CAUTION Do not use the same power supply as that of large equipment such as an air conditioner or centrifuge Malfunction could resu...

Страница 15: ...connecting or disconnecting cables otherwise the equipment may malfunction CAUTION Do not connect any lens or light source other than those recommended by GE Healthcare CAUTION Turn the power switch o...

Страница 16: ...differing types CAUTION Ensure that the Epi lights are connected and locked in position before turning the power on CAUTION Keep the windows of the light sources clean and avoid touching them NOTICE...

Страница 17: ...onnecting the power supply cable Do not tug on the cable and do not handle the connection plugs with wet hands CAUTION Connect the computerhardware on the same power circuit otherwise the equipment ma...

Страница 18: ...d the user documentation before using the system Do not open any covers or replace parts unless specifically stated in the user documentation The system complies with the requirements for electromagne...

Страница 19: ...xcess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concen tration Limits for Certain Hazardous Substances in Electronics Intelligent dark box IDX Camera cable Gett...

Страница 20: ...DX Mechanical shutter Lens mount LAS High Sens lens Lens cap Lens control connector Mount End cap Label Back side F0 85 43mm LAS High Sens Lens 20 Getting Started with ImageQuant LAS 4000 28 9607 59 A...

Страница 21: ...pairs are labelled corresponding to their type Connector Optical filter and LED Illumination LED Distinguishing labels for right and left Retaining screw hole 1 position Guide pin Getting Started wit...

Страница 22: ...wer supply connector Diffusion board UV transilluminator Power supply connector UV glass filter Mercury containing Products Label 22 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 2 Safety ins...

Страница 23: ...lates Sample trays The Epi tray is shown here The White trans tray and UV trans tray are labelled correspondingly Getting Started with ImageQuant LAS 4000 28 9607 59 AB 23 2 Safety instructions 2 2 La...

Страница 24: ...sy to disconnect 2 4 Recycling information The equipment must be clean from contaminants before decommissioning and all local regulations must be observed with regard to waste disposal Disposal genera...

Страница 25: ...ed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of the equipment Getting...

Страница 26: ...table laboratory bench load capacity 100kg m2 Placement At least 20 cm free on all sides 30 to 70 non condensing Humidity Storage 5 to 100 non condensing 3 2 Transport The ImageQuant LAS 4000 weighs a...

Страница 27: ...Note The computer and ImageQuant LAS 4000 must not be connected with a USB cable during the operation 1 Open the control panel and select Printers and Other Hardware 2 Click Add Hardware 3 Click the N...

Страница 28: ...and click the Browse button 10 Select to install the driver from the ImageQuant LAS 4000 CD 11 Open the USB Control folder 12 Select the DevMng inf file and click the Open button 28 Getting Started w...

Страница 29: ...3 Insert the ImageQuant LAS 4000 CD then select Install the software automatically Recommended and click the Next button 4 Click the Finish button to complete the installation 3 4 1 3 Installation of...

Страница 30: ...SB Control Driver Windows VISTA Note The computer and ImageQuant LAS 4000 must not be connected with a USB cable during the operation Note During software installations you may be asked to confirm you...

Страница 31: ...LAS 4000 Control Software CD and click the Browse button 8 Select to install the driver from the ImageQuant LAS 4000 Control Software CD 9 Open the USB Control folder Getting Started with ImageQuant L...

Страница 32: ...inue Enter an administrator password if prompted then click Continue to proceed with the installation 1 Connect the computer and the ImageQuant LAS 4000 with a USB cable and turn ON the power switch o...

Страница 33: ...in the license agreement and click the Next button 5 Select destination folder in the dialog Click the Next button to install the software at the default folder C Program Files Click the Change button...

Страница 34: ...ce of the camera hole Lens release button 2 Attach the two screws fixing the camera head Two screws 3 Connect the camera cable and USB cable USB cable Camera cable 4 Proceed to install the lens See Se...

Страница 35: ...n the loss of image data 3 5 3 Installing the lens This section describes how to install the LAS High Sens lens For instructions on installing the wide view lens refer to the ImageQuant LAS 4000 User...

Страница 36: ...ate it clockwise to lock in position 7 Connect the lens cable Turn the knurling ring until it is securely fixed Connector of the lens cable Knurling ring Note Be sure to connect the lens cable Otherwi...

Страница 37: ...changer position in the ImageQuant LAS 4000 Control Software Note Before installing or changing filters in the filter changer the ImageQuant LAS 4000 Control Software should be installed and running F...

Страница 38: ...instrument door 3 Select Filter Customization in the Option menu in the ImageQuant LAS 4000 Control Software main window 4 Click the Filter down button The filter changer is lowered 5 Open the instrum...

Страница 39: ...ou click the Start Focusing or Method Tray position buttons 3 5 5 Installing a transmitted light source This section describes how to insert the UV transilluminator or the white light table into the I...

Страница 40: ...4 using the ImageQuant LAS 4000 Control Software before attaching the lights as described below Hook Latch Epi light base 1 If the tray is at Tray position 4 proceed to step 3 Otherwise close the IDX...

Страница 41: ...ide it inwards until a click sound is heard 7 Lower the latch to lock the Epi light in place 8 Repeat steps 4 7 for the opposite side Note The Epi lights are clearly labelled L for the left and R for...

Страница 42: ...view The general flow of operation is as follows Turn on the instrument and computer Start the Control Software Place the sample Expose the sample Analyze the image Preparation Exposure 4 2 Preparatio...

Страница 43: ...ns the cooling temperature of the CCD camera is set to 25 C Note The Method Tray position and Focusing may be set even if the CCD is not ready A sample can be placed during the time taken for the CCD...

Страница 44: ...hite Epi light Changing the transmitted light source 1 If no transmitted light source is currently in place inside the instrument proceed to step 4 2 Turn the lever holding the transmitted light sourc...

Страница 45: ...Software before attaching the lights as described below Hook Latch Epi light base 1 If the tray is at Tray position 4 proceed to step 3 Otherwise close the IDX door 2 Click on Method Tray position set...

Страница 46: ...ng open upwards 8 Hook the hanger located on top of the Epi light to the top edge of the Epi light base 1 2 Action No Two guide holes are located on the back of the Epi light base 1 Slide the hook aft...

Страница 47: ...the opposite side Note The Epi lights are clearly labelled L for the left and R for the right sides Install the Epi lights in matching pairs only If Epi lights with different wavelengths are used the...

Страница 48: ...strument and the PC and start the ImageQuant LAS 4000 Control Software 2 Close the instrument door 3 Select Filter Customization in the Option menu in the ImageQuant LAS 4000 Control Software main win...

Страница 49: ...or Method Tray position buttons 4 2 3 Placing the sample This section describes how to choose an appropriate sample tray and how to place the sample for exposure 1 Select a sample tray suitable for t...

Страница 50: ...ut out a gel sheet slightly larger than the sample size b Place the gel sheet on the Trans tray Gel sheet c Place the sample on the gel sheet Gel sheet Sample Note The gel sheet can be used repeatedly...

Страница 51: ...P On the Epi tray there are round dents marked on the tray for positioning the sample Line up the sample using the appropriate dents For NP tray The NP tray is to be used at tray position 3 Note The r...

Страница 52: ...idium bromide EtBr stained sample The general procedure is however similar for all types of sample For detailed instructions refer to the ImageQuant LAS 4000 User Manual Images may also be obtained by...

Страница 53: ...ty Expose Save the image 4 3 2 Choose method and tray position Action Step Click the Method Tray position button 1 Result The Method Tray position dialog opens Getting Started with ImageQuant LAS 4000...

Страница 54: ...escence using Green Epi light 520 nm Fluorescence Cy5 Fluorescence using Red Epi light 630 nm Fluorescence DAPI Fluorescence using NIR Epi light 710 nm Digitization Epi illumination Sample digitizatio...

Страница 55: ...Note Adjusting the brightness does not influence the actual exposure Check that the sample is correctly positioned and focus the image 2 Click the Return button 3 4 3 4 Set exposure type and exposure...

Страница 56: ...ing multiple pixels into one larger pixel Smoothing by interpolation then increases the number of pixels for the final image The sensitivity increase in order Standard High Super and Ultra No pixels f...

Страница 57: ...sure and a digitized white light exposure in order to add an image of an opaque molecular marker with a single click TIP Select Sensitivity Resolution in the Help menu This displays a diagram that des...

Страница 58: ...ntrols at the bottom of the window 1 1 3 2 Function Part Adjust the dynamic range by dragging the limits with the mouse 1 The gradation conversion curve can be toggled between Linear or Sigmoid 2 The...

Страница 59: ...iff format A 16 bit TIFF format suitable for analysis in external software Exposure details are not maintained Click the Complete button 3 Result The current display returns to the initial screen 4 4...

Страница 60: ...CCD will be maintained and you can immediately start using it upon simply restarting Image Quant LAS 4000 Control Software This is useful if more samples will be exposed later in the day Switch off t...

Страница 61: ...oft cloth slightly dampened with a neutral detergent then wipe with a new clean dry cloth To clean the interior of the instrument use a non fluorescent cleanser for biochemical use If using a neutral...

Страница 62: ...the performance to deteriorate Use the protective caps on the camera head and lens whenever they are not installed in the ImageQuant LAS 4000 Store these protective caps carefully Do not allow dirt o...

Страница 63: ...The lights do not remain lit and the tray rail or filter changer do not move when the IDX door is opened Getting Started with ImageQuant LAS 4000 28 9607 59 AB 63 5 Maintenance 5 5 Regular inspections...

Страница 64: ...1 Take note of the error code and error message on the monitor 2 Turn off the power to ImageQuant LAS 4000 and the analysis PC then turn them on again after about ten seconds 3 If the error persists f...

Страница 65: ...d Select the correct light source The wrong light source is selected Expose the Flat Frame again The Flat Frame file is not exposed correctly Place the sample correctly on the sample tray and place th...

Страница 66: ...rn off the ImageQuant LAS 4000 power switch immediately and contact your GE Healthcare representative Camera head failure The camera head generates a strange noise Turn off the ImageQuant LAS 4000 pow...

Страница 67: ...ect the power cable correctly Power cable installation failure LED lights on ImageQuant LAS 4000 do not light during startup diagnosis Remove the foreign object Foreign object is present in the lockin...

Страница 68: ...its value does not approach the set temperature af ter a sufficient time about 15 minutes has elapsed since the start of cooling Set the cooling temperature of the CCD properly The CCD temperature is...

Страница 69: ...ray to the correct position Connect the lens cable correctly Lens cable connection failure No button is displayed in Focusing mode Adjust the focus manually when using the wide view lens The wide view...

Страница 70: ...B020 above are generated during startup diagnosis B0 except described above Lifting table error B101 Filter changer error B102 Focus error B104 Iris error B108 Transmittedlight source error B110 UV tr...

Страница 71: ...D700 Board fan error D800 Camera head fan error D900 Shutter error during exposure DC00 OS system call error E000 6 6 Error messages and countermeasures The tables below describe error messages that...

Страница 72: ...e is write protected Cannot save A file with the same name is write protected Check the correct file is chosen Check the PC is operating cor rectly In the worst case the file may have become corrupted...

Страница 73: ...um exposure time is 30 hours The maximum interval time when using the EtBr method is 60 seconds The maximum interval time when using the UV light is 60 seconds Input a valid filename in the Program Se...

Страница 74: ...ed Please run flat frame calibration for the selected settings Cannot start the exposure Flat frame calibration has not been performed for digitization epi illumination Please run flat frame calibrati...

Страница 75: ...out of the photometric range or a setting may need to be adjusted Reduce the total exposure time so that the UV transilluminator is lit for less than 10 minutes The UV transilluminator was turned off...

Страница 76: ...n Countermeasure Meaning Error message Ensure that the light source is installed correctly The light source could not be identified Cannot identlify the light source Please check that the light unit i...

Страница 77: ...he following settings Light none Filter No1 FUJINON VRF43LMD lens Change the settings appropri ately or perform an exposure in Manual mode The automatic exposure function is unable to calcu late a cor...

Страница 78: ...message Enter a suitable value The supplied value is not appropriate The range of value is 90 110 Please set a suitable value within the interval 78 Getting Started with ImageQuant LAS 4000 28 9607 5...

Страница 79: ...be continuously exposed for up to 30 hours using ImageQuant LAS 4000 Control Software High sensitivity lens F0 85 43 mm Lens Wide view lens F1 8 24 mm Software system Shading correction 250 x 250 mm...

Страница 80: ...x 87 mm Size 200 x 200 mm UV transmitted filter size White LED White transmitted light table 322 x 313 x 87 mm Size 224 x 161 x 252 mm W H D Camera head 510 x 730 x 480 mm W H D IDX External dimension...

Страница 81: ...ecifications of cables required for the use of ImageQuant LAS 4000 100 120 V Voltage 125 V AC 13 A Plug connector SJT3 x 16AWG 60 C Cable Maximum 3 m Power supply cord length 200 240 V Voltage 250 V A...

Страница 82: ...must be complet ed in advance 3 It is not desirable to have a heat source on the IDX right side face where there is an air intake fan even if the environmental requirements have been met 4 Do not ins...

Страница 83: ...it may get wet or flooded 4 Do not install the equipment in an area where it may be exposed to corrosive gas 5 Do not install the equipment in a dusty area 6 Do not install the equipment in an area c...

Страница 84: ...rives More than 1280 1024 pixels Monitor resolution 7 3 Literature For further information on the ImageQuant LAS 4000 and the ImageQuant LAS 4000 Control Software refer to the ImageQuant LAS 4000 User...

Страница 85: ......

Страница 86: ...the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative f...

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