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1.2

Important user information

Read this before operating the
product

All users must read the entire Operating Instructions before installing, operating or
maintaining the product.

Always keep the Operating Instructions at hand when operating the product.

Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.

Intended use

ÄKTA start is a liquid chromatography system used for preparative purification of proteins
at laboratory-scale. The system can be used for a variety of research purposes to fulfill
the needs of the users in the academia and in the life sciences industry.

ÄKTA start is intended for research use only, and shall not be used in any clinical proce-
dures, or for diagnostic purposes.

Prerequisites

In order to follow this manual and use the system in the manner it is intended, it is im-
portant that:

•

you understand the concepts of liquid chromatography

•

you have read and understood the Safety instructions chapter in the ÄKTA start
Maintenance Manual.

ÄKTA start Maintenance Manual 29-0603-08 AC

7

1 Introduction

1.2 Important user information

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Содержание akta start

Страница 1: ...ÄKTA start Maintenance Manual Original instructions ...

Страница 2: ...Page intentionally left blank ...

Страница 3: ...ssories package 55 5 Service and settings 56 5 1 Preventive maintenance 58 5 2 Clean before planned maintenance or service 59 5 3 Access the modules 62 5 4 UV Monitor 64 5 4 1 UV LED calibration 66 5 4 2 Diagnostics 69 5 4 3 Flow cell path length 72 5 4 4 Reset number of run hours 74 5 5 Pump 76 5 5 1 Diagnostics 78 5 5 2 Calibration 80 5 5 3 Pump tubing log 82 5 6 Buffer valve 85 5 7 Sample valve...

Страница 4: ... 133 6 3 Frac30 134 6 4 Pump 135 6 5 Pressure sensor 136 6 6 Main board and Power Supply 138 6 7 System related error messages 139 6 8 Troubleshooting flow charts 145 7 Disassembly 149 7 1 Buffer valve 153 7 2 Mixer 156 7 3 UV 161 7 4 UV flow cell 163 7 5 Sample valve 167 7 6 Pump 172 7 7 Pump tubing 175 7 8 Wash valve 179 7 9 Outlet valve 182 7 10 Conductivity Monitor 186 7 11 Injection valve 189...

Страница 5: ...notices regula tory information intended use of ÄKTA start and list of associated documentation In this chapter See page Section 6 1 1 About this manual 7 1 2 Important user information 9 1 3 Regulatory information 12 1 4 Associated documentation ÄKTA start Maintenance Manual 29 0603 08 AC 5 1 Introduction ...

Страница 6: ...e Fraction collector Frac30 The software installed on a computer UNICORN start The entire liquid chromatography system including instrument Fraction collector and software ÄKTA start System Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in ...

Страница 7: ...raphy system used for preparative purification of proteins at laboratory scale The system can be used for a variety of research purposes to fulfill the needs of the users in the academia and in the life sciences industry ÄKTA start is intended for research use only and shall not be used in any clinical proce dures or for diagnostic purposes Prerequisites In order to follow this manual and use the ...

Страница 8: ...azardous situation which if not avoided could result in minor or moderate injury It is important not to pro ceed until all stated conditions are met and clearly understood NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips A note is used to indicate information that is important for trouble free and optimal use of the product...

Страница 9: ...es AB Name and address of manufacturer Björkgatan 30 SE 751 84 Uppsala Sweden Conformity with EU Directives This product complies with the European directives listed in the table below by fulfilling the corresponding harmonized standards For further information see the EC Declaration of Conformity document Title Directive Machinery Directive MD 2006 42 EC Electromagnetic Compatibility EMC Directiv...

Страница 10: ...trical equipment for mea surement control and labora tory use EN 61010 1 IEC 61010 1 UL 61010 1 CAN CSA C22 2 No 61010 1 EN standard is harmonized with EU directive 2004 108 EC Electrical equipment for measurement control and laboratory use EMC require ments EN 61326 1 IEC 61326 1 Emission according to CISPR 11 Group 1 class A EN ISO standard is harmonized with EU directive 2006 42 EC Safety of ma...

Страница 11: ...tial area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Note Regulatory compliance of connected equipment Any equipment connected to ÄKTA start should meet the safety requirements of EN 61010 1 IEC61010 1 orrelevantharmonizedstandards WithinEU connectedequipment must be CE marked Environmental Conformity Description R...

Страница 12: ...ain ÄKTA start in a safe way ÄKTA start Operating Instructions Dialog descriptions of the functionality menu for ÄKTA start only accessible from the Instrument Display ÄKTA start Instrument Display Help Detailed instrument and module descrip tions including instructions needed to maintain and troubleshoot ÄKTA start ÄKTA start Maintenance Manual A condensed guide to prepare and run chromatographic...

Страница 13: ...rt 1 0 User Manual Dialog descriptions for UNICORN start only accessible from the Help menu UNICORN start Online Help Data files application notes and user documentation on the web To order or download data files application notes or user documentation see the instruc tion below Action Step Go to www gelifesciences com AKTA 1 Click ÄKTA Lab Scale Systems 2 Select ÄKTA start from the list 3 Click R...

Страница 14: ...the code using your mobile phone or tablet computer to access the product page for ÄKTA start Select documents to download under the Related Documents tab 14 ÄKTA start Maintenance Manual 29 0603 08 AC 1 Introduction 1 4 Associated documentation ...

Страница 15: ...n the system and recycling procedures are also described In this chapter This chapter contains the following sections See page Section 16 2 1 Safety precautions 24 2 2 Labels 28 2 3 Emergency procedures 30 2 4 Recycling procedures 31 2 5 Declaration of Hazardous Substances DoHS ÄKTA start Maintenance Manual 29 0603 08 AC 15 2 Safety instructions ...

Страница 16: ...ns WARNING Always follow these General precautions to avoid injury when using ÄKTA start Do not operate ÄKTA start in any other way than described in the ÄKTA start user documentation Operation and user maintenance of ÄKTA start should be per formed according to the instructions described in ÄKTA start Operating Instructions and ÄKTA start Maintenance Manual Do not use any accessories not supplied...

Страница 17: ...w ÄKTA start is filled with denaturatedalcohol 50 C2H5OH ethanol at delivery The denatured alcohol mixture can be hazardous to humans if consumed Flush out the denaturated alcohol before assembling testing or integrating ÄKTA start into the intended process context Personal protection WARNING To avoid hazardous situations when working with ÄKTA start take the following measures for personal protec...

Страница 18: ...d gloves to avoid any circumstances of spillage Cut injuries The tubing cutter is very sharp and must be han dled with care to avoid injuries Hazardous substances When using hazardous chemical and biological agents take all suitable protective measures such as wearing protective glasses and gloves resistant to the sub stances used Follow local and or national regulations for safe operation mainten...

Страница 19: ...ess to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect Installing the computer optional The computer should be installed and used according to the instructions provided by the manufacturer of the computer Disconnect power Always switch off power to ÄKTA start be fore an instrument module is removed or install...

Страница 20: ...When turning or moving the instrument take care not to stretch or squeeze tubing or cables A disconnected cable may cause power interruption or network interruption Stretched tubing may cause bottles to fall resulting in liquid spillage and shattered glass Squeezed tubing may cause in crease in pressure or block liquid flow To avoid the risk of knocking over bottles always place bottles on the buf...

Страница 21: ... cell Make sure that the UV flow cell is pre filled with liquid before starting the system Avoid condensation If ÄKTA start is kept in a cold room cold cabinet or similar keep the instrument switched on in order to avoid condensation Avoid overheating If ÄKTA start is kept in a cold cabinet and the cold cabinet is switched off make sure to switch off the instrument and keep the cold cabinet open t...

Страница 22: ...umen tation Spillage Hazard Avoid spillage of fluids on the surfaces of the instrument which have cables plugs and other wirings Be careful if there is spillage of fluids on the tray while trying to remove the tray from ÄKTA start NaOH is corrosive and therefore dangerous to health When using hazardous chemicals avoid spillage and wear protective glasses and other suitable Personal Protective Equi...

Страница 23: ...ment dry and clean Wipe regularly with a soft damp tissue and if necessary a mild cleaning agent Let the instrument dry completely before use ÄKTA start Maintenance Manual 29 0603 08 AC 23 2 Safety instructions 2 1 Safety precautions ...

Страница 24: ...here Labels on ÄKTA start The illustrations below show the labels that are attached to ÄKTA start Placement Description Label Instrument label including safety symbols and speci fication Do not open any covers on the instrument This will void the warranty Keep the pump cover open when not using the system Open the pump cover after you switch off the instrument 24 ÄKTA start Maintenance Manual 29 0...

Страница 25: ... the Pump before tubing loading Label on Frac30 The illustration below shows the labels that are attached to Frac30 Placement Description Label Instrument label including safety symbols and speci fication Do not open any covers on the instrument This will void the warranty ÄKTA start Maintenance Manual 29 0603 08 AC 25 2 Safety instructions 2 2 Labels ...

Страница 26: ...ed Testing Laboratory NRTL NRTL means an organization that is recognized by the US Occupational Safety and Health Administration OS HA as meeting the legal requirements of Title 29 of the Code of Federal Regulations 29 CFR Part 1910 7 Labels concerning hazardous substances The following symbols on the labels concern hazardous substances Meaning Label This symbol indicates that electrical and elect...

Страница 27: ...s materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Sub stances in Electronic Information Products ÄKTA start Maintenance Manual 29 0603 08 AC 27 2 Safety instructions 2 2 Labels ...

Страница 28: ...ment by pressing the power switch to the O position or by disconnecting the power cord from the instrument The run is interrupted immediately WARNING Access to power switch and power cord with plug Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect 28 ÄKTA start Maintenance Manual 29 0603 ...

Страница 29: ...installed shuts down On the ÄKTA start Instrument Display all four touch buttons will be highlighted The run is interrupted immediately Data generated up to 10 seconds before the power failure can be recovered Note The UNICORN start client may close down during a temporary overload of the processor This may appear as a computer failure The run continues and you can restart the UNICORN start client...

Страница 30: ... according to national and local environmental regulations Recycling of hazardous substances ÄKTA start contains hazardous substances Detailed information is available from your GE representative Disposal of electrical components Waste comprising electrical and electronic equipment must not be disposed of as un sorted municipal waste and must be collected separately Please contact an authorized re...

Страница 31: ...k or mutate under normal operating conditions so that the use of such electronic information products will not result in any severe environmental pollution any bodily injury or damage to any assets The unit of the period is Year In order to maintain the declared EFUP the product shall be operated normally according to the instructions and environmental conditions as defined in the product manual a...

Страница 32: ... limit Meaning Value Indicates that this toxic or hazardous substance contained in all of the homogeneous materials for this part is below the limit requirement in SJ T11363 2006 O 表示该有毒有害物质在该部件所有均质材料中的含量均在SJ T11363 2006 标准规定的限量要求以下 Indicates that this toxic or hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in SJ T11363...

Страница 33: ... 0 0 0 X Frac30 29 0230 511 0 0 0 0 0 0 UNICORN start 29 0187 511 1 The product has not been tested as per the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Product ÄKTA start Maintenance Manual 29 0603 08 AC 33 2 Safety instructions 2 5 Declaration of Hazardous Substances DoHS ...

Страница 34: ...onal Frac30 Fraction col lector For details about the Instrument Display refer to ÄKTA start Operating Instructions In this chapter This chapter contains the following section See page Section 35 3 1 System overview 39 3 2 Instrument 34 ÄKTA start Maintenance Manual 29 0603 08 AC 3 System description ...

Страница 35: ...CORN start software can be used to control ÄKTA start and to analyze the data ac quired during chromatography runs UNICORN start offers several additional features that are described in detail in UNICORN start 1 0 User Manual This section gives an overview of the ÄKTA start System Illustration of the system The illustration below shows the ÄKTA start System with UNICORN start installed on a comput...

Страница 36: ...ng functions Wet side 2 to deliver the liquid in a specified flow path and divert the flow as required to monitor the UV absorbance and conductivity of the liquid Location intended for the placement of buffer bottles used during chromatography runs Buffer tray 3 Connects or disconnects the power Power switch 4 To connect a USB memory stick for storage of results and transfer of files USB port 5 36...

Страница 37: ...ds the tubing used for dispensing the liquid fractions into the collection tubes Tubing holder 2 10 to 18 mm diameter tubes used to collect the fractions Collection tubes 3 Holder for collection tubes which supports tubes of four sizes Bowl assembly 4 Case for electromechanical assembly and holder for the Bowl assembly Base unit 5 Power on indicator LED 6 ÄKTA start Maintenance Manual 29 0603 08 A...

Страница 38: ...niques such as Affinity Chromatography Ion Exchange Chromatography Gel filtration and De salting Quick start methods are available for purifying several common proteins Predefined system methods are available for cleaning the flow path ÄKTA start is operated using a touch screen on the instrument In addition the system can be operated from a computer connectedto the instrument using the UNICORN st...

Страница 39: ... descriptions of the modules placed on the wet side of the instrument 1 2 3 4 5 6 7 8 9 10 Description Function Part A 3 port valve that is used as a switching valve for gradient formation It enables the use of two buffers which are required for forming a gradient during runs Buffer valve 1 ÄKTA start Maintenance Manual 29 0603 08 AC 39 3 System description 3 2 Instrument ...

Страница 40: ...e switches automatically during the predefined cleaning procedure Pump wash In a manual run the valve can be set to the intended position by configuring the run parameters Wash valve 6 A 6 port manually operated valve that is used to transfer the sample loaded in the sample loop on to the column Injection valve 7 A sample loop is connected to the appropriate ports of the valve The valve is switche...

Страница 41: ... cell The cell constant is factory calibrated The Conductivity flow cell is provided with a temperature sensor that measures the tempera ture of the liquid in the Conductivity flow cell Note The buffers used should be within the conductivity range of the instrument 0 to 300 mS cm A 3 port valve that is used to direct the flow to the Fraction collector or to Waste Outlet valve 10 ÄKTA start Mainten...

Страница 42: ...tains the following sections See page Section 43 4 1 Unpack ÄKTA start 48 4 2 Unpack Frac30 54 4 3 Accessories package Introduction This chapter describes how to unpack ÄKTA start and Frac30 Save all the original packing material If the system has to be repacked for transportation or otherwise it is important that the system can be safely packed using the original packing material Note 42 ÄKTA sta...

Страница 43: ... The denatured alcohol mixture can be hazardous to humans if consumed Flush out the denaturated alcohol before assembling testing or integrating ÄKTA start into the intended process context ÄKTA start with packaging weighs about 12 kg No lifting equipment required one person can lift and move the instrument Note Action Step Open the delivery box by cutting the adhesive tape at the top of the box 1...

Страница 44: ... packing Instructions Note Save the documents for future reference 2 Take out the box placed at the top of the package The box contains the accessories delivered with the instrument 3 44 ÄKTA start Maintenance Manual 29 0603 08 AC 4 Unpack ÄKTA start and Frac30 4 1 Unpack ÄKTA start ...

Страница 45: ...old the red strap and then lift the instrument out of the delivery box 4 Open the strap lock and remove the strap 5 ÄKTA start Maintenance Manual 29 0603 08 AC 45 4 Unpack ÄKTA start and Frac30 4 1 Unpack ÄKTA start ...

Страница 46: ...hion from the top of the instrument 6 Remove the foam cushion from the bottom of the instrument by carefully lifting the instrument 7 46 ÄKTA start Maintenance Manual 29 0603 08 AC 4 Unpack ÄKTA start and Frac30 4 1 Unpack ÄKTA start ...

Страница 47: ... Step Remove the plastic bag by gently tilting the system back and forth while pulling out the plastic bag 8 ÄKTA start Maintenance Manual 29 0603 08 AC 47 4 Unpack ÄKTA start and Frac30 4 1 Unpack ÄKTA start ...

Страница 48: ...nser arm This may damage the fraction collector Frac30 with packaging weighs about 6 kg No lifting equipment required one person can lift and move the Fraction collector Note Action Step Open the Frac30 delivery box by cutting the adhesive tape at the top of the box 1 Take out the document placed at the top of the package and read the Un packing Instructions 2 48 ÄKTA start Maintenance Manual 29 0...

Страница 49: ...the fraction collector out of the delivery box Place the Fraction collector on the laboratory bench 3 Open the strap lock and remove the strap 4 ÄKTA start Maintenance Manual 29 0603 08 AC 49 4 Unpack ÄKTA start and Frac30 4 2 Unpack Frac30 ...

Страница 50: ...the top of the Fraction collector 5 Remove the foam cushion from the bottom of the fraction collector by carefully lifting the Fraction collector 6 50 ÄKTA start Maintenance Manual 29 0603 08 AC 4 Unpack ÄKTA start and Frac30 4 2 Unpack Frac30 ...

Страница 51: ...se unit 8 Gently move the Dispenser arm counterclockwise to the end position Push the Drive assembly and hold it at the retracted position At the same time lift the Bowl assembly ÄKTA start Maintenance Manual 29 0603 08 AC 51 4 Unpack ÄKTA start and Frac30 4 2 Unpack Frac30 ...

Страница 52: ...the Base unit 10 Orient the Bowl to match the aligning groove and the aligning features located on the bowl holder Slightly push the Drive assembly laterally and lower the Bowl assembly onto the Base unit 52 ÄKTA start Maintenance Manual 29 0603 08 AC 4 Unpack ÄKTA start and Frac30 4 2 Unpack Frac30 ...

Страница 53: ... or hold Frac30 To lift the module use the handle provided on the bottom plate Do not damage or break the warranty seal label during unpacking of Frac30 Note ÄKTA start Maintenance Manual 29 0603 08 AC 53 4 Unpack ÄKTA start and Frac30 4 2 Unpack Frac30 ...

Страница 54: ...scription Part Unpacking Instructions 1 System certificate 2 Product documentation 3 Maintenance Cue Card 4 System Cue Card 5 CD containing user documentation files and an unpacking video 6 The CD includes Operating Instructions and Maintenance Manual in English and translated versions Accessories box 7 54 ÄKTA start Maintenance Manual 29 0603 08 AC 4 Unpack ÄKTA start and Frac30 4 3 Accessories p...

Страница 55: ...apter This chapter contains the following sections See page Section 56 5 1 Preventive maintenance 58 5 2 Clean before planned maintenance or service 59 5 3 Access the modules 62 5 4 UV Monitor 74 5 5 Pump 82 5 6 Buffer valve 85 5 7 Sample valve 88 5 8 Wash valve 91 5 9 Outlet valve 94 5 10 Conductivity Monitor 101 5 11 Pressure sensor 103 5 12 Frac30 109 5 13 Instrument Display 118 5 14 System 127...

Страница 56: ... is not going to be used for a few days prepare the system for storage Daily Visually inspect the inlet filters and clean them if necessary Weekly or when required Cleaning procedure immerse and leave the inlet filters in 1 M NaOH for about 2 hours Clean the system flow path with 0 5 to 1 M NaOH and rinse with demineralized water Note Do not leave the UV flow cell in NaOH for more than 20 minutes ...

Страница 57: ...leaning Pump wash A and B Washout fractionation tubing Clean the UV flow cell Clean the Conductivity flow cell When required Calibrate the Pump Weekly or when required Calibrate the UV flow cell When required Calibrate the Conductivity flow cell When required Perform Zero offset calibration for the Pressure sensor When required Replace the inlet filters When required Replace the tubing and connect...

Страница 58: ...ety Declaration Form must be completed before this is started Please complete the checklist in the On Site Service Health Safety Declaration Form or the Health Safety Declaration Form for Product Return or Servicing depending on whether the instrument is going to be serviced on site or returned to GE for service re spectively Copy the form you need from Section 8 1 Health and Safety Declaration Fo...

Страница 59: ... 7 8 9 10 Service settings instructions Module Part Instructions on page 82 Buffer valve 1 Mixer 2 Instructions on page 85 Sample valve 3 Section 5 5 Pump on page 74 Pump 4 Instructions on page 101 Pressure sensor 5 Instructions on page 88 Wash valve 6 Injection valve 7 ÄKTA start Maintenance Manual 29 0603 08 AC 59 5 Service and settings 5 3 Access the modules ...

Страница 60: ... valve 10 Instructions Maintenance and service of the different modules are operated from the Instrument Display For instructions about maintenance of a module see the specific sections in this chapter Action Step Tap Settings and service to access different modules for parameter setting or maintenance 1 60 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 3 Access the modules ...

Страница 61: ...Next or Back to move through the screens 1 to 3 Tap the intended module button to select a specific module for maintenance 2 ÄKTA start Maintenance Manual 29 0603 08 AC 61 5 Service and settings 5 3 Access the modules ...

Страница 62: ...ration 66 5 4 2 Diagnostics 69 5 4 3 Flow cell path length 72 5 4 4 Reset number of run hours Access the UV Monitor options Follow the instructions below to access the options for calibrating and troubleshooting the UV Monitor Action Step In the Settings and service screen tap Next Next to access the 3rd screen 1 Result The following screen opens 62 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Se...

Страница 63: ...Action Step Tap UV to access the UV Monitor options 2 Result The UV screen opens ÄKTA start Maintenance Manual 29 0603 08 AC 63 5 Service and settings 5 4 UV Monitor ...

Страница 64: ...uctions below to calibrate the UV LED Action Step Make sure that 1 The UV flow cell is assembled tightly The inlet and outlet tubing and the fingertight connectors are properly tightened to block any stray light from entering the UV flow cell The system is not exposed to direct sunlight Flush the UV flow cell with demineralized water using the Pump Make sure that there are no air bubbles in the UV...

Страница 65: ...Signal response of 2500 mV 5 If the Signal value is above 2500 mV tap Save If the Signal value is below 2500 mV use the arrows to increase the Light strength until the Signal is above 2500 mV Tap Save 6 Result The calibrated Light strength value is saved and stored in the perma nent memory on main board ÄKTA start Maintenance Manual 29 0603 08 AC 65 5 Service and settings 5 4 UV Monitor 5 4 1 UV L...

Страница 66: ...e Absorbance Abs should have a value 2000 mAU Stray light test Check for electrical noise Follow the instructions below to perform a Dark current test Action Step Make sure that 1 The UV flow cell is assembled tightly The inlet and the outlet tubing and connectors are tightened to block any stray light from entering The system is not exposed to direct sunlight Flush and fill the UV flow cell with ...

Страница 67: ...ss the UV Monitor is working as it should Fail replace the UV Monitor Check for presence of stray light Follow the instructions below to perform a Stray light test Action Step Flush and fill the UV flow cell with 15 acetone using a syringe Make sure that there are no air bubbles in the syringe 1 ÄKTA start Maintenance Manual 29 0603 08 AC 67 5 Service and settings 5 4 UV Monitor 5 4 2 Diagnostics ...

Страница 68: ...nected tubing Make sure that the detector is not exposed to e g direct sunlight If the test fails again replace the flow cell and then possibly the UV Monitor or contact a GE Service Engineer Flush the system with demineralized water so that the Abs value returns close to zero 3 68 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 4 UV Monitor 5 4 2 Diagnostics ...

Страница 69: ...bsorbance of the liquid in the UV flow cell mAU Abs Ambient temperature compensation constant C amb UV LED drift compensation constant C drft NOTICE UV Configuration of C amb and C drft values must be performed by a GE Service Engineer The C amb and C drft values should not be altered by a user Prerequisites Buffer A immerse Buffer A inlet into demineralized water Buffer B freshly prepared 1 0 ace...

Страница 70: ...rrows or enter the value in the text box 3 Flush the UV flow cell thoroughly and leave it filled with demineralized water 4 Tap Set baseline to capture a new reference value for the test Result Abs should show a value close to 0 mAU Flush the UV flow cell with 1 0 acetone solution and then leave it filled 5 Note the new Abs value Calculate the actual Flow cell path length by using the following fo...

Страница 71: ...the value to permanent memory Note The Abs value should be 340 5 mAU confirming that normalization has been done 7 Note For higher precision instead of using acetone use the Fe2 SO4 3 kit product number 18 1129 63 ÄKTA start Maintenance Manual 29 0603 08 AC 71 5 Service and settings 5 4 UV Monitor 5 4 3 Flow cell path length ...

Страница 72: ...e performed by a GE Service Engineer The C amb and C drft values should not be altered by a user After replacing the UV Monitor it is required to reset the number of run hours of UV Monitor Note Action Step Tap Configuration 1 Result The Configuration screen opens 72 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 4 UV Monitor 5 4 4 Reset number of run hours ...

Страница 73: ... screen opens If the UV Monitor has been replaced tap Yes 3 Or If the UV Monitor has not been replaced tap No to cancel the action ÄKTA start Maintenance Manual 29 0603 08 AC 73 5 Service and settings 5 4 UV Monitor 5 4 4 Reset number of run hours ...

Страница 74: ...ctions See page Section 76 5 5 1 Diagnostics 78 5 5 2 Calibration 80 5 5 3 Pump tubing log Access the Pump service options Follow the instruction below to access the options for Pump calibration and troubleshoot ing Action Step In the Settings and service screen tap Pump 1 Result The Pump screen opens 74 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 5 Pump ...

Страница 75: ...Action Step Tap to select the intended option 2 ÄKTA start Maintenance Manual 29 0603 08 AC 75 5 Service and settings 5 5 Pump ...

Страница 76: ...w rate Follow the instructions below to perform Pump diagnostics Action Step Immerse the buffer inlet tubing A in demineralized water 1 In the Pump screen tap Diagnostics 2 Result The Diagnostics screen opens Use the up down arrows to set the desired Flow Rate value in the range 0 5 to 5 ml min 3 76 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 5 Pump 5 5 1 Diagnostics ...

Страница 77: ...ed collection tube containing the collected water and estimate the volume of the pumped water Calculate the flow rate and verify that it corresponds to the set flow rate Note If the collected water does not correspond to the required volume i e the Pump does not deliver water with the intended flow rate inspect the condition of the pump tubing then re calibrate the Pump and re diagnose 6 If the co...

Страница 78: ...o calibrate the Pump Action Step Immerse the buffer inlet tubing A in demineralized water Place the outlet tubing from the Wash valve in a pre weighed collection tube Note Before starting the calibration prime the flow path with water and make sure that the outlet tubing where the pumped water is collected is filled with demineralized water This ensures that the volume of the collected water corre...

Страница 79: ...cted water weigh the pre weighed collection tube containing the collected water and calculate the volume of the pumped water and then set the Collected Volume value equal to this volume 4 Tap Calibrate 5 Result The following screen opens Wait while the calibration is running The calibration is done when the Cali bration screen re opens 6 ÄKTA start Maintenance Manual 29 0603 08 AC 79 5 Service and...

Страница 80: ...s to 0 Tubing run Instructions Follow the instructions below to check the Pump tubing log and to reset the number of hours the pump tubing has been used Action Step Tap Pump tubing log 1 Result The Pump tubing log screen opens In the Pump tubing log screen tap Reset 2 Result A confirmation screen opens 80 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 5 Pump 5 5 3 Pump tubing...

Страница 81: ... Yes if the pump tubing has been replaced 3 Or If the pump tubing has not been replaced tap No to cancel the action ÄKTA start Maintenance Manual 29 0603 08 AC 81 5 Service and settings 5 5 Pump 5 5 3 Pump tubing log ...

Страница 82: ...s This option is used for troubleshooting the valve Valve switches Instructions Follow the instructions below to check if the Buffer valve functions properly Action Step Immerse the buffer inlet tubing A and B in demineralized water Place the outlet tubing from the Wash valve in the waste bottle 1 Start the Pump 2 In the Settings and service screen access Pump Diagnostics screen Enter the desired ...

Страница 83: ...hat the flow is delivered from the Buffer A inlet or the Buffer B inlet according to the selected valve position Note The Buffer valve is by default in the Buffer A position the liquid is delivered from the Buffer A inlet 4 Visually inspect the flow at the outlet 5 If the water is not flowing properly check the following and fix accordingly Is there a blockage in the tubing Are the connectors tigh...

Страница 84: ...en replaced tap Reset to set the Valve switches counter to 0 8 Result A confirmation screen opens Tap Yes to confirm the reset if the valve has been replaced Or If the valve has not been replaced tap No to cancel the action 84 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 6 Buffer valve ...

Страница 85: ... for troubleshooting the valve Valve switches Instructions Follow the instructions below to check if the Sample valve functions properly Action Step Immerse the buffer inlet tubing A and the sample inlet tubing in deminer alized water Place the outlet tubing from the Wash valve in the waste bottle Check that Buffer A is selected in the Buffer valve screen 1 Start the Pump 2 In the Settings and ser...

Страница 86: ...y There is also a click sound when the valve switches Check that the flow is delivered from the Buffer inlet or the Sample inlet according to the selected valve position Note The Sample valve is by default in the Buffer position the liquid is delivered from the Buffer inlet 5 Visually inspect the flow at the outlet If the water is not flowing properly check the following and fix accordingly 6 Is t...

Страница 87: ...e is faulty replace the Sample valve 8 If the valve has been replaced tap Reset to set the Valve switches counter to 0 9 Result A confirmation screen opens Tap Yes to confirm the reset if the valve has been replaced Or If the valve has not been replaced tap No to cancel the action ÄKTA start Maintenance Manual 29 0603 08 AC 87 5 Service and settings 5 7 Sample valve ...

Страница 88: ...ches Instructions Follow the instructions below to check if the Wash valve functions properly Action Step Immerse the buffer inlet tubing A in demineralized water Place the outlet tubing from the Wash valve and the outlet tubing from the Outlet valve in the waste bottle Check that Buffer A is selected in the Buffer valve screen Buffer is selected in the Sample valve screen 1 Start the Pump 2 In th...

Страница 89: ...aste outlet or the Column outlet according to the selected valve position Note The Wash valve is by default in Waste position the flow is diverted to waste 4 Visually inspect the flow at the outlet If the water is not flowing properly check the following and fix accordingly 5 Is there a blockage in the tubing Are the connectors tightly connected Stop the Pump 6 In the Settings and service screen a...

Страница 90: ... switches counter to 0 8 Result A confirmation screen opens Tap Yes to confirm the reset if the valve has been replaced Or If the valve has not been replaced tap No to cancel the action 90 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 8 Wash valve ...

Страница 91: ...Valve switches Instructions Follow the instructions below to check if the Outlet valve functions properly Action Step Immerse the buffer inlet tubing A in demineralized water Place the outlet tubing from the Outlet valve in the waste bottle Check that Buffer A is selected in the Buffer valve screen Buffer is selected in the Sample valve screen Column is selected in the Wash valve screen 1 Start th...

Страница 92: ...e flow is diverted to the Waste outlet or the Fraction col lector outlet according to the selected valve position Note The Wash valve is by default in Waste valve position Hence the flow is di verted to Waste 4 Visually inspect the flow at the outlet If the water is not flowing properly check the following and fix accordingly 5 Is there a blockage in the tubing Are the connectors tightly connected...

Страница 93: ...en replaced tap Reset to set the Valve switches counter to 0 8 Result A confirmation screen opens Tap Yes to confirm the reset if the valve has been replaced Or If the valve has not been replaced tap No to cancel the action ÄKTA start Maintenance Manual 29 0603 08 AC 93 5 Service and settings 5 9 Outlet valve ...

Страница 94: ...has a temperature sensor for measuring the temperature of the liquid in the cell Description Parameter The Cell constant is a characteristicof the conduc tivity cell defined as the ratio of the distance be tween the electrodes and the area of an electrode Cell constant Set cell const Ambient temperature variations influence the conductivity In Handbooks and other documen tation the conductivity va...

Страница 95: ...tant value Action Step In the Settings and service screen tap Next Next to access the 3rd screen 1 Result The following screen opens Tap Conductivity Result The Conductivity screen opens Tap Configuration 2 Result The Configuration screen opens ÄKTA start Maintenance Manual 29 0603 08 AC 95 5 Service and settings 5 10 Conductivity Monitor 5 10 1 Set the Cell constant value ...

Страница 96: ...alibration on page98 Set the reference temperature For the system to recalculate the measured conductivity to conductivity at a certain reference temperature enter the temperature in the Set ref temp field Note Make sure that the checkbox Enable temperature compensation in the Conductivity Calibration screen is checked Note Action Step In the Conductivity Configuration screen set the reference tem...

Страница 97: ...mineralized water through the system with a flow rate of 0 5 ml min 1 In the Conductivity screen tap Advanced calibration 2 Result The Advanced calibration screen opens Note the temperature and enter it into the Set actual temp text box and then tap Calibrate to carry out the temperature calibration Note Make sure that the temperature of the Conductivity flow cell has stabi lized and measure the t...

Страница 98: ... disable temperature compensation tap the Enable temperature compen sation checkbox in the Calibration screen Note Action Step Fill the Conductivity flow cell with conductivity standard solution 1 In the Conductivity screen tap Calibration 2 Result The Calibration screen opens Note Make sure that the checkbox for Enable Temperature Compensation is checked 98 ÄKTA start Maintenance Manual 29 0603 0...

Страница 99: ...bration Note If a certified conductivity standard solution is used use the supplied theo retical conductivity value corresponding to the temperature in question If a manually prepared 1 00 M NaCl calibration solution is used get the conductivity value at the current temperature from the graph for conduc tivity of the 1 00 M NaCl as a function of temperature presented below 4 ÄKTA start Maintenance...

Страница 100: ...ction Step Description Axis Temperature C x Conductivity mS cm y 100 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 10 Conductivity Monitor 5 10 3 Conductivity flow cell calibration ...

Страница 101: ...pressure only Zero offset NOTICE Calibration of Pressure sensor must be performed by a GE Service Engineer and must be done when the Pressure sensor has been replaced Action Step Disconnect the inlet tubing from the Pressure sensor to expose the sensor to atmospheric pressure only Note Make sure that the Pump is OFF before disconnecting the tubing 1 ÄKTA start Maintenance Manual 29 0603 08 AC 101 ...

Страница 102: ...t The Pressure sensor screen opens In the Pressure sensor screen tap Zero offset 3 Result A Message screen opens Make sure that there is no back pressure in the system and then tap OK 102 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 11 Pressure sensor ...

Страница 103: ...erform diagnostics and handle the run log In this section This section contains the following subsections See page Section 104 5 12 1 Enable or disable Frac30 105 5 12 2 Diagnostics 107 5 12 3 Run Log ÄKTA start Maintenance Manual 29 0603 08 AC 103 5 Service and settings 5 12 Frac30 ...

Страница 104: ...Result If the Fraction collector is enabled the following Fraction collector screen opens If the Fraction collector is not enabled the following screen opens Tap Enable Frac to enable the Fraction collector 2 Or To disable the Fraction collector tap Disable Frac 104 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 12 Frac30 5 12 1 Enable or disable Frac30 ...

Страница 105: ... the current position to the home position tube number 1 Home test Instructions Follow the instructions below to run a diagnostics test on Frac30 Action Step In the Fraction collector screen tap Diagnostics 1 Result The Diagnostics screen opens Tap Feed tube test and visually observe that Frac30 rotates from one tube to the next 2 ÄKTA start Maintenance Manual 29 0603 08 AC 105 5 Service and setti...

Страница 106: ...ac30 does not rotate the Bowl assembly check if the Frac30 cable is connected properly to ÄKTA start If the cable is not connected properly connect the cable properly and secure it in position with a screw driver if required 3 106 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 12 Frac30 5 12 2 Diagnostics ...

Страница 107: ...urs of drive sleeve usage Run log Instructions Follow the instructions below to reset the Run log Action Step In the Fraction collector screen tap Run log 1 Result The Run log screen opens ÄKTA start Maintenance Manual 29 0603 08 AC 107 5 Service and settings 5 12 Frac30 5 12 3 Run Log ...

Страница 108: ... to 0 2 Result A confirmation screen opens Tap Yes to confirm the reset if the drive sleeve has been replaced Or If the drive sleeve has not been replaced tap No to cancel the action 108 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 12 Frac30 5 12 3 Run Log ...

Страница 109: ...en calibration 114 5 13 2 Color test 115 5 13 3 Diagnostics 116 5 13 4 Log book Instructions Follow the instructions below to select an option to calibrate and or diagnose the Instru ment Display Action Step In the Settings and service screen tap Next Next to access the 3rd screen 1 Result The following screen opens ÄKTA start Maintenance Manual 29 0603 08 AC 109 5 Service and settings 5 13 Instru...

Страница 110: ...Action Step Tap Display to access the Instrument Display options 2 Result The Display screen opens 110 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 13 Instrument Display ...

Страница 111: ...n Step In the Display screen tap Touch screen calibration 1 Result A warning screen opens Tap Yes to proceed with the calibration of the Touch screen 2 Result The following screen opens ÄKTA start Maintenance Manual 29 0603 08 AC 111 5 Service and settings 5 13 Instrument Display 5 13 1 Touch screen calibration ...

Страница 112: ... opens Tap precisely on the marked circle 2 4 Result The following screen opens Tap precisely on the marked circle 3 5 Result The following screen opens 112 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 13 Instrument Display 5 13 1 Touch screen calibration ...

Страница 113: ...llowing screen opens Tap Exit Note If the calibration fails repeat the test If the calibration fails again contact a GE Service Engineer 7 ÄKTA start Maintenance Manual 29 0603 08 AC 113 5 Service and settings 5 13 Instrument Display 5 13 1 Touch screen calibration ...

Страница 114: ...en Action Step In the Display screen tap Color test 1 Result The following screen opens Tap Exit 2 Result The Color test is completed Note If the test fails contact a GE Service Engineer 114 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 13 Instrument Display 5 13 2 Color test ...

Страница 115: ...The following screen opens In the Select backlight brightness screen tap a radio button to select the intended brightness 2 Result The rectangle shows the backlight brightness at the selected bright ness level Tap Back to return to the Display screen 3 ÄKTA start Maintenance Manual 29 0603 08 AC 115 5 Service and settings 5 13 Instrument Display 5 13 3 Diagnostics ...

Страница 116: ... usage The instrument display must be replaced by a GE Service Engineer Note Instructions Follow the instructions below to read the Log book for the Display Action Step In the Display screen tap Log book 1 Result The following screen opens Note the number of hours the Instrument Display has been used 116 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 13 Instrument Display 5 1...

Страница 117: ...2 Result A confirmation screen opens Tap Yes to confirm the reset if the Instrument Display has been replaced Or If the Instrument Display has not been replaced tap No to cancel the action ÄKTA start Maintenance Manual 29 0603 08 AC 117 5 Service and settings 5 13 Instrument Display 5 13 4 Log book ...

Страница 118: ...of the UV Monitor and the collection tubes This option is used to set the delay volume ml Delay volume setting This option is used to update the firmware version of the instrument whenever a new firmware is available on the ÄKTA start product support page refer to www gelifesciences com AKTA Firmware update ÄKTA start has a dedicated digital drive mecha nism to improve the life of modules This is ...

Страница 119: ...tim ing to any value from 3 0 to 5 0 sec 0 1 sec increments For exporting details on the running condition of the UV Pump pump tubing all the solenoid valves and the latest 4 error messages with Error codes to a USB memory stick The latest Firmware ver sion and FPGA version can also be exported Export system report to USB The data is used by a GE Service Engineer when troubleshooting the instrumen...

Страница 120: ...c files located on the USB memory stick Download the latest AKTASTRT src file from the product support page onto the USB memory stick refer to www gelifesciences com AKTA Instructions Follow the instructions below to update the firmware Action Step Plug the USB memory stick into the USB port located on ÄKTA start 1 In the System screen tap Firmware update 2 Result The following screen opens 120 ÄK...

Страница 121: ...strument automatically restarts and displays the version of the firmware From the product support page www gelifesciences com AKTA download AKTASTRT dat for FPGA update 4 Tap FPGA update 5 Result The following screen opens Tap Yes The updating may take up to 5 minutes 6 Result The following screen opens ÄKTA start Maintenance Manual 29 0603 08 AC 121 5 Service and settings 5 14 System 5 14 1 Firmw...

Страница 122: ... calibration Pressure sensor Zero offset Pump Calibration Flow rate UV UV LED calibration Conductivity Advanced calibration temperature sensor calibration Conductivity Calibration flow cell calibration 122 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 14 System 5 14 1 Firmware update ...

Страница 123: ...to a com puter 3 Check the content of the system report files INSTLOG TXT and ERRORLOG TXT see section System report file parameters 4 System report files parameters The content of the INSTLOG TXT file contains the following parameters ÄKTA start serial no Firmware version FPGA version Pump run Pump tube run UV LED run time Buffer valve count Wash valve count Outlet valve count Sample valve count ...

Страница 124: ...in the respective fields and then tap Save 2 Result The total delay volume from the UV Monitor to Frac30 is displayed in the Total Delay volume field Note The delay volume from the UV Monitor to the Outlet valve is constant 0 27 ml in all ÄKTA start instruments Delay volume setting needs to be set when the tube between Outlet valve and Frac30 has been replaced Note 124 ÄKTA start Maintenance Manua...

Страница 125: ...lowing screen opens In the System screen tap Switch valve timing 2 Result The following screen opens Tap the radio button to select Switch valve timing B switch time 5 sec 3 Tap Save to save the timing Perform Gradient run either by performing System performance method or manually set the B concentration Buffer valve to 50 Observe for the fluctuations 4 ÄKTA start Maintenance Manual 29 0603 08 AC ...

Страница 126: ...elect Advanced timing 5 Result The following screen opens Set switch valve time in the range of 3 0 to 5 0 sec 0 1 sec increments by pressing the up down arrows Tap Save to save the optimized timing 6 126 ÄKTA start Maintenance Manual 29 0603 08 AC 5 Service and settings 5 14 System 5 14 4 Switch valve timing ...

Страница 127: ...ations performed by a GE Service Engineer only Do not perform any further operations from this screen Tap Back or Home to return to the previous screen or to go to the Home screen ÄKTA start Maintenance Manual 29 0603 08 AC 127 5 Service and settings 5 15 Main board ...

Страница 128: ...r Supply 138 6 7 System related error messages 139 6 8 Troubleshooting flow charts Introduction For ÄKTA start and Frac30 there are two kinds of Troubleshooting procedures Basic Application related troubleshooting For instructions refer to section Basic Troubleshooting in ÄKTA start Operating Instructions Troubleshooting with the aid of Warning messages and Error codes displayed on the Touch Scree...

Страница 129: ...lower UV light strength max 500 counts Tap Calibrate to get a signal at about 2500 mV In the Settings and ser vice UV screen When trying to save the UV light strength after calibration if the signal is greater than 4000 mV UV intensi ty high 112 Order a new UV Moni tor Perform UV LED cali bration For instructions see In the Settings and ser vice UV screen When trying to save the UV light strength ...

Страница 130: ... cali brations and the signal strength is decreasing Flush UV flow cell and mount securely 115 Flush the UV flow cell and mount it in a se cure way Try to recalibrate If the voltage is still less than 2500 mV change UV module In the Settings and ser vice UV screen Calibration and run time When the UV signal is not in the range of 2500 mV and 4000 mV auto zero cannot be performed UV base line ig no...

Страница 131: ... to the detec tor UV module failure 102 Troubleshooting Flow chart 2 on page 140 See UV module cable may be loose or disconnected no communication with UV module Main PWA failure UV module failure 103 Troubleshooting Flow chart 3 on page 141 ÄKTA start Maintenance Manual 29 0603 08 AC 131 6 Troubleshooting 6 1 UV ...

Страница 132: ...se Descrip tion Error code See Loose cable connec tor The temperature sen sor is not functioning Conductivi ty module failure 201 Troubleshooting Flow chart 6 on page 144 132 ÄKTA start Maintenance Manual 29 0603 08 AC 6 Troubleshooting 6 2 Conductivity ...

Страница 133: ...ÄKTA start and Frac30 is not working Drive sleeve worn out Sensors are not work ing The Frac30 internal cable is not working Motor failure Main board failure Fraction collector failure 301 Troubleshooting Flow chart 4 on page 142 ÄKTA start Maintenance Manual 29 0603 08 AC 133 6 Troubleshooting 6 3 Frac30 ...

Страница 134: ...mp tubing Check that there is no blockage on the inlet or outlet Open the pump head and visually check if the pump rollers are rotating freely when starting the Pump from the Diagnostics screen If not check if the Pump connector is connected or if any thing is stuck between the rollers The rollers are not rotat ing No flow from the Pump 134 ÄKTA start Maintenance Manual 29 0603 08 AC 6 Troubleshoo...

Страница 135: ...n sep aration media with suitable solution 1 M NaOH or replace the column with a new one Check the specifica tion and pressure of the columns for cor rect flow rate Blockagein the tubing valves or in the col umn Improperly selected Flow rate Wrong pressure set ting used for chosen application Overpres sure 501 Perform Zero offset Contact a GE Service Engineer The sensor is not connect ed or not ca...

Страница 136: ... sun light System operation is not affected by this warning the system can continue to be used Instrument tempera ture is out of range 4 C to 35 C System over tem perature 011 Error messages Action Possible cause Descrip tion Error code Contact a GE Service Engineer EPROM er ror 001 Contact a GE Service Engineer The system cannot be used in this condition Instrument tempera ture is way out of rang...

Страница 137: ...vice Engineer With assistance from a GE Service Engineer check the Power sup ply for voltages Voltage has gone out of operating range Power sup ply monitor error 003 ÄKTA start Maintenance Manual 29 0603 08 AC 137 6 Troubleshooting 6 6 Main board and Power Supply ...

Страница 138: ...01 Wrong method loaded Reload the method Incomplete method Illegal op code Reload Method 602 Wrong method loaded Restart the system Wrong operations in the system User is trying to work on features that are not supported IllegalOper ation Restart In strument 603 138 ÄKTA start Maintenance Manual 29 0603 08 AC 6 Troubleshooting 6 7 System related error messages ...

Страница 139: ...rform UV LED calibration Error message still displayed 1 Replace the Flow cell Follow the Flow cell replacement procedure 2 Tap Continue on the error display 3 Perform UV LED calibration Switch instrument Off then On again Error message still displayed 1 Switch the instrument Off 2 Disassemble the UV Monitor 3 Replace the UV Monitor and Flow cell 4 Switch instrument On Error message still displaye...

Страница 140: ... Error message still displayed Switch instrument Off then On again Error message still displayed 1 Replace the Flow cell Follow the Flow cell replacement procedure 2 Tap Continue on the error display 1 Switch instrument Off 2 Disassemble the UV Monitor 3 Replace the UV Monitor and Flow cell 4 Switch instrument On Perform UV LED calibration Proceed with your work Contact GE Service Engineer Note Th...

Страница 141: ...mble the UV Monitor 3 Check that the UV Monitor RJ45 cable is secure replug properly 4 Switch on the instrument Error message still displayed Proceed with your work Contact GE Service Engineer Yes No ÄKTA start Maintenance Manual 29 0603 08 AC 141 6 Troubleshooting 6 8 Troubleshooting flow charts ...

Страница 142: ...Drive sleeve worn out Check Proceed with your work Replace the Drive sleeve Error message still displayed Does the Motor work properly Contact GE Service Engineer Contact GE Service Engineer Proceed with your work Run Frac30 diagnostics Perform indexing of homing to check if Motor works properly Note This Error message is displayed only when Frac30 is operated Yes No No No No Yes Yes Yes No Yes 14...

Страница 143: ...Troubleshooting Flow chart 5 ERROR 401 Pump driver Failure Contact GE Service Engineer ÄKTA start Maintenance Manual 29 0603 08 AC 143 6 Troubleshooting 6 8 Troubleshooting flow charts ...

Страница 144: ...ble the Conductivity Monitor Switch on the instrument 1 Replace the Conductivity Monitor 2 Enter the new Cell Constant value 3 Perform Conductivity calibration Error message still displayed Proceed with your work Has calibration passed Contact GE Service Engineer No Yes Yes No 144 ÄKTA start Maintenance Manual 29 0603 08 AC 6 Troubleshooting 6 8 Troubleshooting flow charts ...

Страница 145: ...ins the following sections See page Section 149 7 1 Buffer valve 153 7 2 Mixer 156 7 3 UV 161 7 4 UV flow cell 163 7 5 Sample valve 167 7 6 Pump 172 7 7 Pump tubing 175 7 8 Wash valve 179 7 9 Outlet valve 182 7 10 Conductivity Monitor 186 7 11 Injection valve 189 7 12 Injection valve kit 192 7 13 Frac30 Bowl assembly 200 7 14 Fuse ÄKTA start Maintenance Manual 29 0603 08 AC 145 7 Disassembly ...

Страница 146: ...hen performing maintenance on ÄKTA start follow the instructions below Electrical shock hazard Do not open any covers or parts unless specified in the user documentation Except for the mainte nance and service described in the user documentation all other repairs should be done by a GE Service Engineer Disconnect power Always switch off power to the instrument before replacing any component on the...

Страница 147: ... instructions Module Part Instructions on page 149 Buffer valve 1 Instructions on page 153 Mixer 2 Instructions on page 163 Sample valve 3 Instructions on page 167 Pump 4 Pressure sensor 5 Instructions on page 175 Wash valve 6 Instructions on page 186 Injection valve 7 Instructions on page 156 UV 8 ÄKTA start Maintenance Manual 29 0603 08 AC 147 7 Disassembly ...

Страница 148: ...Disassembly instructions Module Part Instructions on page 182 Conductivity 9 Instructions on page 179 Outlet valve 10 148 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly ...

Страница 149: ... below to remove and replace the Buffer valve WARNING Disconnect power Always switch off power to the instrument be fore replacing any component on the instrument or cleaning the instrument unless stated otherwise in the user documentation Action Step Switch off the instrument by pressing the Power Switch to the O position 1 Remove the inlet and outlet tubing from the valve ports 2 ÄKTA start Main...

Страница 150: ...t at delivery Note The screw can be located on different places on the top circumference of the holder 3 Slowly remove the Buffer valve until a cable is visible The cable is assembled with two connectors interconnected 4 150 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 1 Buffer valve ...

Страница 151: ... connector and remove the connector and cable 5 Disconnect the connector from the valve side 6 Remove the valve and leave the connector as it is 7 ÄKTA start Maintenance Manual 29 0603 08 AC 151 7 Disassembly 7 1 Buffer valve ...

Страница 152: ... that the part number P N on the new valve matches with that of the old valve 29003271 Buffer valve CTV 31 516U 1 8 Make sure that the ports are aligned to the markings on the instrument chassi 9 Switch on the instrument by pressing the Power Switch to the l position 10 152 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 1 Buffer valve ...

Страница 153: ...witch off power to the instrument be fore replacing any component on the instrument or cleaning the instrument unless stated otherwise in the user documentation Action Step Switch off the instrument by pressing the Power Switch to the O position 1 Remove the inlet and outlet tubing from the ports 2 ÄKTA start Maintenance Manual 29 0603 08 AC 153 7 Disassembly 7 2 Mixer ...

Страница 154: ...ction Step Loosen the M4 screw from the Mixer using a T20 screwdriver supplied with the equipment at delivery 3 Remove the Mixer 4 154 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 2 Mixer ...

Страница 155: ...p Replace with a new Mixer and reconnect the tubing to the ports 5 Switch on the instrument by pressing the Power Switch to the l position 6 ÄKTA start Maintenance Manual 29 0603 08 AC 155 7 Disassembly 7 2 Mixer ...

Страница 156: ... switch off power to the instrument be fore replacing any component on the instrument or cleaning the instrument unless stated otherwise in the user documentation Action Step Switch off the instrument by pressing the Power Switch to the O position 1 Remove the inlet and outlet tubing from the ports 2 156 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 3 UV ...

Страница 157: ...ew the one screw at the top of the UV Monitor and then the two screws at the bottom using a T20 screwdriver supplied with the equipment at deliv ery 3 ÄKTA start Maintenance Manual 29 0603 08 AC 157 7 Disassembly 7 3 UV ...

Страница 158: ...ess the latching tab and disconnect the RJ45 connector from the UV Monitor side Note Make sure that the RJ45 connector is not moved inside the cabinet The con nector needs to be retained outside the cabinet for re assembling of a new UV module 5 158 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 3 UV ...

Страница 159: ...ect the RJ45 connector to the new UV monitor 7 Note Make sure that there is a click sound when the RJ45 connector is connected The UV monitor may not work properly if the RJ45 connector is not properly connected ÄKTA start Maintenance Manual 29 0603 08 AC 159 7 Disassembly 7 3 UV ...

Страница 160: ...Action Step Reconnect the tubing to the ports 8 Switch on the instrument by pressing the Power Switch to the l position 9 160 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 3 UV ...

Страница 161: ...t the inlet and outlet tubing from the UV Monitor 1 Rotate the locknut in the anticlockwise direction 2 Pull up the UV flow cell 3 Put a new UV flow cell in place from above so that it fits in properly and si multaneously tighten the locknut 4 ÄKTA start Maintenance Manual 29 0603 08 AC 161 7 Disassembly 7 4 UV flow cell ...

Страница 162: ...mbled after completing the assembly of the UV flow cell inside the UV Monitor by just press fit In the Settings and Service screen tap UV In the UV screen perform a UV LED calibration and a Flow cell path length test 6 This is described further in UV LED Calibration and Flow cell path length test 162 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 4 UV flow cell ...

Страница 163: ...switch off power to the instrument be fore replacing any component on the instrument or cleaning the instrument unless stated otherwise in the user documentation Action Step Switch off the instrument by pressing the Power Switch to the O position 1 Remove the inlet and outlet tubing from the ports 2 ÄKTA start Maintenance Manual 29 0603 08 AC 163 7 Disassembly 7 5 Sample valve ...

Страница 164: ...at delivery Note The screw can be locate on different places on the top circumference of the holder 3 Slowly remove the Sample valve until a cable is visible The cable is assem bled with two connectors interconnected 4 164 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 5 Sample valve ...

Страница 165: ...Action Step Disconnect the connector from the valve side 5 Remove the Sample valve 6 ÄKTA start Maintenance Manual 29 0603 08 AC 165 7 Disassembly 7 5 Sample valve ...

Страница 166: ...ure that the part number P N on the new valve matches with that of the old valve 29003272 Sample valve CTV 31 32U 3 Make sure that the ports are aligned to the markings on the instrument chassi 8 Switch on the instrument by pressing the Power Switch to the l position 9 166 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 5 Sample valve ...

Страница 167: ...strument or cleaning the instrument unless stated otherwise in the user documentation NOTICE Keep the pump cover open when not using the system Open the peristaltic pump cover after you switch off the equipment This re duces the risk of shortening the life time of the pump tubing Action Step Switch off the instrument by pressing the Power Switch to the O position 1 ÄKTA start Maintenance Manual 29...

Страница 168: ...Action Step Open the top cover to remove the tubing from the Pump 2 168 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 6 Pump ...

Страница 169: ...he connector needs to be retained outside the cabinet for re assembling of a new Pump module 3 Note While loosening the screw make sure to hold the screw in your hand before it is completely out of the Pump There is a risk that the screw will fall into the pump head if proper care is not taken during the disassembly ÄKTA start Maintenance Manual 29 0603 08 AC 169 7 Disassembly 7 6 Pump ...

Страница 170: ...y remove the Pump until a cable is visible The cable is assembled with two connectors interconnected 4 Disconnect the connector from the Pump side 5 170 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 6 Pump ...

Страница 171: ... not get stuck in the ventilation fan Replace with a new Pump install the new Pump in reverse order 7 Place the Pump tubing between the rollers and the track of the new Pump 8 Switch on the instrument by pressing the Power Switch to the l position 9 In the Pump screen tap Diagnostics and then reset the number of hours of Pump run to 0 10 ÄKTA start Maintenance Manual 29 0603 08 AC 171 7 Disassembl...

Страница 172: ...the instrument be fore replacing any component on the instrument or cleaning the instrument unless stated otherwise in the user documentation Action Step Switch off the instrument by pressing the Power Switch to the O position 1 Open the top cover until it is fully open 2 172 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 7 Pump tubing ...

Страница 173: ...ke sure that the pump tubing is not twisted or stretched against the rollers Lower the top cover until it clicks into its fully closed position 4 The track closes automatically and the tubing is stretched correctly as the track closes ÄKTA start Maintenance Manual 29 0603 08 AC 173 7 Disassembly 7 7 Pump tubing ...

Страница 174: ...ct the pump tubing to the Sample valve and to the Pressure sensor 5 Switch on the instrument by pressing the Power Switch to the l position 6 174 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 7 Pump tubing ...

Страница 175: ...witch off power to the instrument be fore replacing any component on the instrument or cleaning the instrument unless stated otherwise in the user documentation Action Step Switch off the instrument by pressing the Power Switch to the O position 1 Remove the inlet and outlet tubing from the ports 2 ÄKTA start Maintenance Manual 29 0603 08 AC 175 7 Disassembly 7 8 Wash valve ...

Страница 176: ...pment at delivery Note The screw can be located on different places on the top circumference of the holder 3 Slowly remove the Wash valve until a cable is visible The cable is assembled with two connectors interconnected 4 176 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 8 Wash valve ...

Страница 177: ...hen reconnect the tubing to the valve ports 7 Note Before replacing the valve make sure that the part number P N on the new valve matches with that of the old valve 29014870 AA Wash valve CTV 3 32UH 2 Make sure that the ports are aligned to the markings on the instrument chassi 8 ÄKTA start Maintenance Manual 29 0603 08 AC 177 7 Disassembly 7 8 Wash valve ...

Страница 178: ...Action Step Switch on the instrument by pressing the Power Switch to the l position 9 178 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 8 Wash valve ...

Страница 179: ...switch off power to the instrument be fore replacing any component on the instrument or cleaning the instrument unless stated otherwise in the user documentation Action Step Switch off the instrument by pressing the Power Switch to the O position 1 Remove the inlet and outlet tubing from the ports 2 ÄKTA start Maintenance Manual 29 0603 08 AC 179 7 Disassembly 7 9 Outlet valve ...

Страница 180: ...ment at delivery Note The screw can be located on different places on the top circumference of the holder 3 Slowly remove the Outlet valve until a cable is visible The cable is assembled with two connectors interconnected 4 180 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 9 Outlet valve ...

Страница 181: ... Note Before replacing the valve make sure that the part number on the new valve matches with that of the old valve 29003274 Outlet valve CTV 31 32U 1 7 Make sure that the ports are aligned to the markings on the instrument chassi 8 Switch on the instrument by pressing the Power Switch to the l position 9 ÄKTA start Maintenance Manual 29 0603 08 AC 181 7 Disassembly 7 9 Outlet valve ...

Страница 182: ...mponent on the instrument or cleaning the instrument unless stated otherwise in the user documentation If required twist the connector to bring the connector out of the instrument hole before disassembly the Conductivity Monitor Note Action Step Switch off the instrument by pressing the Power Switch to the O position 1 Remove the inlet and outlet tubing from the ports 2 182 ÄKTA start Maintenance ...

Страница 183: ... T20 screwdriv er supplied with the equipment at delivery 3 Slowly remove the Conductivity Monitor until a cable is visible The cable is assembled with two connectors interconnected 4 ÄKTA start Maintenance Manual 29 0603 08 AC 183 7 Disassembly 7 10 Conductivity Monitor ...

Страница 184: ...Monitor and leave the connector as it is Note Make sure that the connector is not moved inside the cabinet The connector needs to be retained outside the cabinet for re assembling of a new Conduc tivity module 6 184 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 10 Conductivity Monitor ...

Страница 185: ... pressing the Power Switch to the l position 8 In the Settings and service screen access the Conductivity screen to set the cell constant for the new Conductivity flow cell For details see Chapter 5 Service and settings on page 55 9 ÄKTA start Maintenance Manual 29 0603 08 AC 185 7 Disassembly 7 10 Conductivity Monitor ...

Страница 186: ... switch off power to the instrument be fore replacing any component on the instrument or cleaning the instrument unless stated otherwise in the user documentation Action Step Switch off the instrument by pressing the Power Switch to the O position 1 Remove the inlet and outlet tubing from the ports 2 186 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 11 Injection valve ...

Страница 187: ...n the M3 screw from the Injection valve using a T10 torx driver sup plied with the equipment at delivery 3 Remove the Injection valve 4 ÄKTA start Maintenance Manual 29 0603 08 AC 187 7 Disassembly 7 11 Injection valve ...

Страница 188: ...the tubing to the ports 5 Make sure that the ports are aligned to the markings on the instrument chassi 6 Switch on the instrument by pressing the Power Switch to the l position 7 188 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 11 Injection valve ...

Страница 189: ...Follow the instructions below to remove and replace the Injection valve kit Action Step Make sure that the valve is in position Inject and then disconnect the inlet and outlet tubing from the ports 1 ÄKTA start Maintenance Manual 29 0603 08 AC 189 7 Disassembly 7 12 Injection valve kit ...

Страница 190: ...x driver Loosen each screw equally in turn so the distribution plate comes off in parallel to the valve body 2 Slide the screws out 3 Remove the distribution plate containing the ports 4 190 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 12 Injection valve kit ...

Страница 191: ...ution plate so that the marks on the plate match the marks on ÄKTA start Using the torx driver tighten the 3 screws in turn a little at a time until the distribution plate is fixed to the valve body 6 ÄKTA start Maintenance Manual 29 0603 08 AC 191 7 Disassembly 7 12 Injection valve kit ...

Страница 192: ...move the Frac30 Bowl assembly Action Step Gently move the Dispenser arm counterclockwise to the non dispensing end position 1 Push the drive assembly laterally and hold it at the retracted position 2 192 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 13 Frac30 Bowl assembly ...

Страница 193: ...onto the Bowl holder Follow the instructions below to mount the Frac30 Bowl assembly onto the Bowl holder Action Step Make sure that the Dispenser arm is in the non dispensing position 1 ÄKTA start Maintenance Manual 29 0603 08 AC 193 7 Disassembly 7 13 Frac30 Bowl assembly ...

Страница 194: ... Step Note the position of the aligning groove on the Bowl holder 2 Note the position of the aligning ribs on the Bowl 3 194 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 13 Frac30 Bowl assembly ...

Страница 195: ...the Dispenser arm Tip If it is hard to locate the alignment feature in corresponding parts hold the Bowl assembly near the top of the Bowl holder and rotate the Bowl holder unless the aligning ribs gets located After locating the aligning features leave the Bowl to slide freely into the Bowl holder 5 ÄKTA start Maintenance Manual 29 0603 08 AC 195 7 Disassembly 7 13 Frac30 Bowl assembly ...

Страница 196: ...n Step Hold the Drive assembly at the retracted position to completely assemble the Bowl assembly on the Bowl holder 6 196 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 13 Frac30 Bowl assembly ...

Страница 197: ...ow the instructions below to remove and replace the drive sleeve Action Step Cut the old drive sleeve with a cutter and then remove the drive sleeve 1 ÄKTA start Maintenance Manual 29 0603 08 AC 197 7 Disassembly 7 13 Frac30 Bowl assembly ...

Страница 198: ...o the drive 2 Remove and fix the Tubing holder Follow the instructions below to remove and replace the Tubing holder Action Step Pull out the Tubing holder from the Dispenser arm 1 198 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 13 Frac30 Bowl assembly ...

Страница 199: ...Step Press down the new Tubing holder into the Dispenser arm until it is stopped by the dispensing arm stopping feature 2 ÄKTA start Maintenance Manual 29 0603 08 AC 199 7 Disassembly 7 13 Frac30 Bowl assembly ...

Страница 200: ...Follow the instructions below to remove the Fuse Action Step Use the flat screwdriver provided and push the Snap feature on the left side of the Fuse holder in the direction indicated by the arrow mark 1 200 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 14 Fuse ...

Страница 201: ... feature on the right side of the Fuse holder in the direction indicated by the arrow mark 2 It should now be possible to slide out the Fuse from the holder pull out the Fuse by hand 3 ÄKTA start Maintenance Manual 29 0603 08 AC 201 7 Disassembly 7 14 Fuse ...

Страница 202: ...n Step Align the Fuse holder guide feature to the rectangular slot of the Mains filter 1 Push the Fuse holder into the rectangular slot until it fits into the rectangular groove of the Mains filter 2 202 ÄKTA start Maintenance Manual 29 0603 08 AC 7 Disassembly 7 14 Fuse ...

Страница 203: ...udes Health and Declaration forms for service and ordering information for ÄKTA start In this chapter See page Section 204 8 1 Health and Safety Declaration Form 206 8 2 Ordering information ÄKTA start Maintenance Manual 29 0603 08 AC 203 8 Reference information ...

Страница 204: ...abeled en removed fr above has bee onnel and our c ce Engineer st e Service Engi may lead to de swer Yes or ers in box belo ous substance scanner surfac ument is clean ed to allow saf re customer to have been rem e instrument from the area Seria en cleaned to Comp Date GE and GE GE Healthc NJ 08855 1 2010 14 On Site Safety customers all arts a repair T neer upon arr lays in servicin No ow es ces o...

Страница 205: ...n please specify service retu e system equ Wate Ethan None Argo Liqui lease d above has b ervice numbe er service Healt for Pr and or Service Ticket nel our custom aminants befo with your retu cing or return return to GE m d additional cl n in contact wi ces please sp y rn Please pro uipment er nol e empty n Helium Nit d Nitrogen Seria been cleaned t Comp instit Date er GE and GE GE Healthc NJ 088...

Страница 206: ... 60 Mixer ÄKTA start Mixer 29 0240 18 UV module ÄKTA start UV 29 0113 25 Flow Cell 2 mm UPC 900 29 0240 21 Conductivity Cell ÄKTA start Conductivity 18 1120 39 Sample Loop PEEK 10 µl Sample loops 18 1113 98 Sample Loop 100 µl INV 907 18 1113 99 Sample Loop 500 µl INV 907 18 1114 01 Sample Loop 1 0 ml INV 907 18 1114 02 Sample Loop 2 0 ml INV 907 18 1140 53 Sample Loop 5 ml PEEK 18 1161 24 Sample L...

Страница 207: ...emale for 1 16 o d tubing titanium 11 0003 39 Union Valco F F 18 1127 66 Fill port 29 0240 32 Inlet tubing Kit ÄKTA start Tubing 29 0240 34 Complete tubing kit ÄKTA start 18 1112 53 PEEK tubing i d 0 75 mm 1 16 18 1115 83 PEEK tubing i d 1 0 mm 1 16 18 1113 68 PEEK tubing 2 m i d 0 5 mm o d 1 16 19 2447 01 Mains cable 115 V Cables 19 2448 01 Mains cable 220 V 29 0240 36 Cable Assy OTH USB ÄKTA sta...

Страница 208: ...ox 29 0270 57 Operating Instructions printed 29 0603 08 Maintenance Manual printed 18 1110 89 Injection kit 29 0187 51 UNICORN start DVD license access code and manual package Software 29 0230 51 Frac30 Assembly Frac30 19 6067 02 Drive sleeve 18 6464 01 Tubing holder 29 0240 45 Bowl Assembly Frac30 29 0240 65 Cable Assembly Frac30 ÄKTA start spare parts Code no Item 29 0320 87 Packaging Kit for ÄK...

Страница 209: ...rvice tools Code no Item 29 0031 71 Torx driver T10 28 9513 03 Torx driver T20 56 4656 00 Flat screwdriver ÄKTA start Maintenance Manual 29 0603 08 AC 209 8 Reference information 8 2 Ordering information ...

Страница 210: ... 37 mount bowl assembly 193 remove and fix drive sleeve 197 remove and fix tubing hold er 198 remove bowl assembly 192 reset Run log 107 settings and diagnos tics 103 Fuse replace 200 I Injection valve remove and replace 186 remove and replace kit 189 Installation Unpack Frac30 48 Instrument Display calibration and tests 109 Color test 114 diagnostics 115 display Log book 116 Touch screen calibra ...

Страница 211: ...ve functional checks 85 remove and replace 163 Service and settings maintenance schedule 56 System error messages 138 export system report 123 firmware updates 120 set Switch valve timing 125 set the delay volume 124 T Troubleshooting 128 conductivity monitor 132 flow charts 139 Frac30 133 main board 136 power supply 136 Pressure sensor 135 Pump 134 system errors 138 UV monitor 129 Typographical c...

Страница 212: ...Page intentionally left blank ...

Страница 213: ...Page intentionally left blank ...

Страница 214: ...ble on request Contact your local GE Healthcare repre sentative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hy...

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