GE HEALTHCARE Biacore T100, Руководство пользователя

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Biacore T100 Software Handbook BR-1006-48 Edition AE
205
Affinity in solution 11
11.3 Evaluation of affinity in solution
To evaluate affinity in solution measurements, open the result file and click
Affinity in Solution on the toolbar. The first step displays the calibration curve
for measurement of free B:
Choose the sensorgram, report point, response type and fitting function from
the pull-down lists at the top of the dialog. See Section 8.2.1 for more details of
these choices.
If you have run multiple sample series in the experiment, choose the sample to
evaluate in the
Sample list. A sample series is defined as all cycles with the same
sample name in the assay step(s) with purpose Sample .
If you have run multiple calibration curves in the experiment, choose the curve
to use in the
Calibration curve list. A calibration curve is defined as
measurements from assay step(s) with purpose Calibration , regardless of the
sample name. If two or more Calibration assay steps are run contiguously with
no intervening steps with a different purpose, they will be combined into a single
calibration curve.
Note: All samples in a series are evaluated against the chosen calibration curve.
You cannot use different calibration curves for different samples in the
same series.
The table lists the data for the calibration curve. The plot panel shows the curve
with calibration points as black inverted triangles and sample points as red
squares. Samples that lie outside the range of the calibration curve are not
shown. Right click on calibration points to exclude the points from the curve.