Biacore T100 Software Handbook BR-1006-48 Edition AE
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Data presentation tools 7
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Each report point in the plot (including the actual control sample
responses) is adjusted so that it retains the same position relative to the
positive and negative controls before and after adjustment.
Adjustment for controls cannot be applied in regions where the positive control
curve lies below the negative control curve (or below the x-axis if no negative
control is selected). Any points that lie in such regions will be excluded from the
adjusted plot.
Adjustment for controls applies only to the current plot and does not affect any
other evaluation item.
Note:
Beware of using a polynomial fitting function with less than 4 control
samples. The parabolic curve created by the function can deviate greatly
from the points, leading to adjustment that does not reflect the drift in the
control responses (Figure 7-2).
Figure 7-2.
Polynomial function fitted to 3 control points (highlighted) with the resultant
adjustment for controls.
Содержание Biacore T100
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Страница 214: ...Appendix A Data import and export A 2 Importing data 214 Biacore T100 Software Handbook BR 1006 48 Edition AE ...
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