5 Methods
5.6 Cycle types
88
Biacore T100 Software Handbook BR-1006-48 Edition AE
To set up a conditional command:
1
Insert an
If…then
command at the appropriate place in the cycle definition.
2
Specify the condition. This is defined as the outcome of a comparison
between a report point value (absolute response, relative response or slope)
and a constant or another report point value with an added or subtracted
constant value. Only report points that have already been set in the cycle
definition may be used in the condition.
Check
Use additional condition
to combine two conditions, using either
AND (both conditions must be fulfilled) or OR (fulfillment of one condition is
sufficient) as a logical operator.
The available comparison conditions are
Greater than
and
Less than
. The
conditions do not include
Equal to
since exact equality is an unpredictable
condition in view of noise in the SPR response. To construct an equality
condition, combine one
Greater than
and one
Less than
condition so that
a window of tolerance is created. For example, the combined condition
A
greater than B-1 AND A less than B+1
is equivalent to
A equals B
with a
tolerance of
±
1.
3
Choose the actions to be taken when the condition is met and when it is not
met. You may choose to execute a command sequence, terminate the
cycle or the method, or introduce a stabilization period.
If you choose a command sequence for either the
True
or
False
outcome,
click on the appropriate branch of the command (
Then
or
Else
respectively)
and insert the commands you wish to be executed. If you leave the branch
empty, the cycle will simply continue with the next command following the
If…then
construction.
You can use the
Move up
and
Move down
buttons to rearrange the order
of commands within a branch, but you cannot move commands outside
the branch in which they are placed.
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