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NT8 User's Guide | Chapter 9: Sitting Drop Experiments
●
Next to
Action
, click
Aspirate
.
●
Next to
Column
, select the columns you want to aspirate from, and enter the
volume to be aspirated. To select multiple columns, hold down the
SHIFT
key and
click the columns you want to select.
●
Click the well area in the
Select Target
picture to select it (highlighted in yellow
at right).
●
Select a
Liquid Class
from the liquid class list.
●
If you are done adding commands for that plate, click
Add
. If you will dispense to
drop locations on this plate, click
Add More
.
7.
Instruct the NT8 to dispense the protein and well solution mixture to the drop location
on the microplate.
●
If you clicked
Add More
in the last step, the Task Details dialog box remained
open. If not, double-click the microplate image again to open it.
●
Next to
Action
, click
Dispense
.
●
Next to
Column
, select the columns you want to dispense to. Next to
Volume
,
enter the volume to be dispensed. If you want the NT8 to mix your solution, enter
the number of mixing cycles next to
Mixing
(optional).
●
Select a
Liquid Class
from the liquid class list.
●
Next to
Z-Offset
, you may choose to let the NT8’s Flexible Fingers determine the
dispense height (recommended), or manually specify the distance you want the
fingers to stop at before dispensing.
●
Click the drop location in the
Select Target
area (drop 1 is selected in the image
at right).
8.
Click
End Repeat
in the Task List to select it.
9.
Add the
Dispose Tip
command to the Task List by clicking the
Waste Station
image.
10.
Optional
: Save your Task List by clicking the
Save
button .
Add Protein to the Protein Block
●
Using a hand pipette, pipette the protein to be used in the sitting drop experiment
into the protein block.
Run the Task List
●
Click the
Run Task List
button to execute the Task List. The NT8 picks up the LV
tips, washes them, aspirates from the protein block, aspirates from the wells in the
microplate, and then dispenses to the drop location. This process repeats for each
column you specified earlier. After the NT8 has completed the tasks for each column,
it disposes the tips in the waste station.
