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NT8 User's Guide | Chapter 6: Initial Setup
Note:
An asterisk indicates the liquid class that was selected when you created the Aspirate
command.
Adding the LV or HV Dispose Tip Command
To add the LV Dispose Tip command, take the following steps:
●
Double-click the
Waste Station
to add the Dispose Tip command to the Task List.
To add the HV Dispose Tip command, take the following steps:
1.
Make sure that the HV Pick Tip command appears in the Tasks Lists panel.
2.
Double-click the
HV Tip Caddy Station
to add the Dispose Tip command to the
Task List.
Selecting the correct liquid class for your protein and well solutions results in more precise
dispenses, as the NT8 has been programmed to handle liquids of different viscosities
according to their specific properties.
Use the information below to help you select the correct liquid class.
Liquid Class Guidelines
Liquid Class
Examples
Normal (N)
Almost all buffers, salt solutions, and other aqueous solutions.
Medium (M)
20% MPD, Lysozyme protein, viscous solutions with viscosities
at or below 50% PEG 4K.
High (H)
70% MPD, 50% PEG 5K.
Very High (V)
Viscous solutions with viscosities at or below 50% PEG 6K or
higher and 100% glycerol.
Note:
The table above is for well solutions only; protein viscosity classes differ. Normal
to High viscosity for a protein sample is equal to lysozyme 20 mg/mL to 100 mg/mL.
