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8

NT8 User's Guide | Chapter 4: Overview

LCP Dispensing

The NT8’s LCP option can accurately and quickly dispense drops as low as 30 nL. Its fully-
automated calibration system ensures that the syringe tip is centered to within 50 µm of the
target drop location, and it can dispense LCP and well solution drops to a 96-drop LCP plate in
about 7 minutes.

Active Humidification

The NT8 prevents sample evaporation with three powerful, fully-enclosed humidifiers that
actively control humidity from ambient to 95%. You can set humidity on/off manually or “set it
and forget it” and let the NT8 do the work.

The NT8 can increase humidity from 60% to 85% in under 20 seconds. The humidifiers are
positioned away from active samples so your drops are protected from condensation and
buildup.

User-Friendly Software

The NT8 software is designed to be user-friendly. See

Software Overview

 for further details.

The NT8 can be configured for LCP dispenses and for plate copying.

LCP

The lipid cubic phase (LCP) option includes a dispensing attachment that can dispense LCP
drops as small as 25-200 nL. The dispenser uses an 8 mm barrel syringe, which a sensor
monitors for proper positioning before dispensing begins.

The LCP model dispenses a complete 96-drop LCP plate in fewer than 6 minutes for both the
lipid drop setting well solution drop setting.

LCP models include a lipid mixer and an automatic LCP calibration system. The lipid mixer
attaches to the NT8’s deck, and is controlled by the software to ensure the right number of
mixing cycles are applied to the syringe. The automatic calibration system ensures the syringe
tip is centered to within 50 µm.

Plate Copy

The plate copy option includes a specialized head that includes a mount for HV tips. The plate
copy head can aspirate and dispense up to 200 µL of reagent from a deep well block to a
crystallization plate. The plate copy head consists of eight positive displacement syringes
capable of dispensing viscous reagents.

The NT8 deck can accommodate one rack of standard 200 µL pipette tips and can
automatically load and eject tips. While most tips are washable, plate copy tips are not and
must be disposed of after use.

Options

Содержание NT8

Страница 1: ...NT8 User s Guide Original Instructions Applies to LCP Plate Copy Options Third Edition Revised June 2018 NTG V263R115 2018 Formulatrix All Rights Reserved ...

Страница 2: ...com For support email support formulatrix com To comment on Formulatrix documentation email publications formulatrix com Formulatrix European Representative Flip Hoedemaeker Support Europe Phone 31 652 451106 flip formulatrix com Acknowledgements The NT8 was developed in collaboration with Vadim Cherezov of The Scripps Research Institute TSRI and the JCIMPT Center led by Ray Stevens and supported ...

Страница 3: ...t Safety Guidelines 4 Mechanical Hazards 4 Electrical Hazards 4 Specifications 5 Dimensions 5 Weight 5 Computer Requirements 5 Electrical Requirements 6 Overview 7 Key Features 7 Options 8 Hardware Overview 9 The NT8 Deck 9 The NT8 Head 11 Initial Setup 13 Setting Up the NT8 13 Preparing the NT8 for a Dispense 15 ...

Страница 4: ...tting Drop Experiments 42 Hanging Drop Experiments 45 Creating Daughter Plates Plate Copying 50 Mixing LCP Drops 53 LCP Experiments 56 Running Rock Maker Experiments with NT8 60 System Maintenance 62 Sterilizing the Liquid Lines 62 Lubricating the Plate Copy Plungers 64 Lubricating the O rings 67 Enabling the Programmatic Tab 68 Troubleshooting and FAQs 70 Aspiration or Dispense Problems 70 NT8 Do...

Страница 5: ... s Guide or use of the products in violation of applicable laws rules or regulations Except as otherwise expressly provided in this user guide any alteration adjustment or modification to the products will void the product warranty Read understand and observe all safety information and instructions in this manual before using your NT8 Please note specific safety requirements as explained in this u...

Страница 6: ...es a caution relating to electric shock and advises you of precautions to take This icon denotes a fire hazard and advises you of precautions to take This icon denotes a warning Bold text denotes items you must select or click in the software such as menu items and dialog box options Bold text also denotes parameter names bold Text in this font denotes text or characters you should enter from the ...

Страница 7: ...rmulatrix products are CE Conformité Européenne Machinery Low Voltage and EMC directive compliant If necessary request the official Declaration of Conformity DOC from Formulatrix The product is also CDRH Chapter 21 CFR 1040 10 compliant Regulatory Compliance Introduction Only Formulatrix personnel are permitted to transport assemble or service the NT8 Use only parts supplied or recommended by Form...

Страница 8: ...lectromechanical device Only persons with the proper training should service or operate the product All facilities to the product must be disconnected before servicing or injury may result from the automatic operation of the equipment The proper precautions for operating electrical equipment must be observed Equipment Safety Guidelines Mechanical Hazards Electrical Hazards Avoid damage to the powe...

Страница 9: ...ould be placed under the table Ambient Temperature Range 4 C to 30 C Operating Humidity 0 95 non condensing Storage Humidity 0 95 non condensing 653 mm x 511 mm x 431 mm 6 9 x 8 5 x 9 3 38 kg 83 8 lb Windows 7 Windows Vista or Windows XP 1 open USB Port Dimensions Weight Store the NT8 within the specified ranges of temperature and humidity where it is not exposed to direct sunlight Computer Requir...

Страница 10: ... Hz 60 Hz 130 W typical 200 W max Standard or European electrical outlet Electrical Requirements Confirm that the power supply requirements are properly met to avoid material damage and fire The power supply for the system must meet or exceed electrical requirements of the system to avoid damage and risk of fire ...

Страница 11: ...ck to the crystallization plate all eight tips must touch the plate To achieve this each tip is attached to a flexible finger that can travel 0 5 mm vertically enabling the dispense head to contour to each dispense surface Each tip also has a sensor that indicates when the tip contacts a surface These sensors allow the NT8 to drive down precisely to the point where all tips touch the plate Plate C...

Страница 12: ...P option includes a dispensing attachment that can dispense LCP drops as small as 25 200 nL The dispenser uses an 8 mm barrel syringe which a sensor monitors for proper positioning before dispensing begins The LCP model dispenses a complete 96 drop LCP plate in fewer than 6 minutes for both the lipid drop setting well solution drop setting LCP models include a lipid mixer and an automatic LCP cali...

Страница 13: ...the head and deck Both are located at the front of the NT8 The head primarily contains tools to aspirate and dispense liquids and the deck contains plates tip caddies the protein source and the wash and waste stations Located at the front of the instrument the NT8 deck contains everything needed for your dispenses including the plates protein source and tips The NT8 Deck ...

Страница 14: ...T8 for washing low volume pipette tips 4 Low Volume LV Tip Caddy Station The low volume LV tip caddy station is a repository that holds up to 384 replacement LV tips 5 6 7 Microplate Stations On the deck of the NT8 there are designated areas for three SBS footprint microplates Three microplates can be placed on the deck to transfer reagents from one plate to another A hanging drop cover sheet can ...

Страница 15: ...or will be off and bright blue otherwise see the picture below Optional High Volume HV Tip Attachments HV tips are located at the bottom right of the NT8 s head Each HV tip attachment is equipped with a sensor that detects any existing liquid in a deep well plate The HV tip attachment uses 200 µL pipette tips eight at a time to aspirate liquid from a deep well block and transfer it to a crystalliz...

Страница 16: ...ver Adjusts the LCP syringe mount Optional Lipidic Cubic Phase LCP Syringe Mount Compatible with Hamilton LCP syringe Note You can adjust the lever speed and height to fit the LCP syringe by clicking on the toolbar and moving the lever button on the NT8 s head up and down as necessary ...

Страница 17: ... your computer A diagram of the setup is shown below for reference To set up the NT8 for initial use 1 Prepare the water and waste carboys Please note that the water carboy must only be filled with distilled water Place the water carboy near the NT8 and the waste carboy under the table Power Outlet Computer Laptop USB Cable Wash Tank Waste Tank Power Cable Setting Up the NT8 ...

Страница 18: ...ice Manager in Windows and click Continue if Windows User Active Control is active If you DO NOT have the FTDI driver on your computer your NT8 will show up twice as NT8V3 1 with a yellow warning symbol Proceed to step 9 If you DO have the FTDI driver installed your NT8 will show up As USB Serial Converter A and USB Serial Converter B Proceed to Step 10 9 Install the FTDI driver Right click the NT...

Страница 19: ...up each component for a dispense so make sure to read the quick start guide before prepping your hardware to avoid unnecessary hardware setup Prerequisites You are familiar with the NT8 hardware This tutorial covers the following topics Installing the LV Protein Block 1 Make sure that you have attached the sample blocks to the protein source 2 Place the protein source on the protein source station...

Страница 20: ... Installing the Reference Plate 1 Put the reference plate on the desired microplate station 2 Make sure the arrow mark position is on the top right corner Installing the HV Tip Caddy 1 Click the button to move the NT8 s head to the left and stage back This will allow you to slide the HV tip caddy into place 2 Slide the tip caddy into position 1 2 H ...

Страница 21: ...microplate station 2 You have installed a low volume LV tip into the LV tip attachment 1 You have the microplate data sheet that came with your plate Creating a Plate Definition 1 Right click on any plate in the Plate Type Library and select New Plate Type The Plate Type Editor dialog box appears 2 Follow the on screen instructions presented in the wizard 3 When you have finished following all the...

Страница 22: ...e Plate Type Library into the HV tip caddy station or LV tip caddy station 2 Set up the tips a Right click on the HV tip caddy station or LV tip caddy station b Select Configure HV Tip Caddy or Configure LV Tip Caddy c Select the appropriate column numbers on the tip caddy setup dialog box 3 Click OK 4 Double click the HV tips or LV tips to add the Pick Tip command to the Task List Adding Commands...

Страница 23: ...he corresponding field or click the up or down arrows to increase or decrease the wash time Setting the Protein Volume 1 Right click the protein station and select Set Protein Volume 2 Select the location of your protein sample in the protein station This should correlate to where your protein sample will be in the protein block 3 Enter or select a volume in the Volume box 4 Click OK ...

Страница 24: ...l drop volume of solution you wish to aspirate 9 Select the appropriate protein liquid class from the Liquid Class list see Liquid Class Guidelines 10 Click Add Adding a Plate and Setting the Aspirate Volume 1 Drag and drop the appropriate plate for your experiment from the Plate Type Library to the desired microplate station 2 Double click the microplate to set the aspirate details 3 From the SEL...

Страница 25: ...HEAD field first before configuring LV head setup 3 Select the columns that you want to dispense to In the example above we selected the first well columns 1 12 4 Optional Click on the long arrow below the dispense radio button to reverse the dispense direction This step is required for hanging drop experiments 5 Select the desired target drop located in the lower left of the dialog by clicking on...

Страница 26: ...t liquid class for your protein and well solutions results in more precise dispenses as the NT8 has been programmed to handle liquids of different viscosities according to their specific properties Use the information below to help you select the correct liquid class Liquid Class Guidelines Liquid Class Examples Normal N Almost all buffers salt solutions and other aqueous solutions Medium M 20 MPD...

Страница 27: ... Prerequisites You want to perform the LCP drop dispense An LCP syringe Installing the LCP Syringe Before you perform the LCP drop dispense you ll need to install the parts To install the LCP syringe take the following steps 1 Install the LCP syringe by inserting it into the LCP holder slot 2 Push the LCP syringe to make sure it is firmly attached to the syringe holder 3 After the plunger has been...

Страница 28: ...the humidifier When the humidity level reaches the limit the system automatically turns off the humidifier 3 Click Save Occasionally you might want to empty and clean the system liquid and humidifier chamber that serve your NT8 To do so you ll need to turn the pump control settings off while you re cleaning the chambers and then turn them back on Prerequisites Humidifier is on manual mode click on...

Страница 29: ... following steps 4 Click on the toolbar to start draining the chambers 5 Clean the chambers To fill the system liquid and humidifier chambers take the following steps 1 Click on the toolbar to fill the chambers 2 If you want to return to Automatic Humidifier control click the Automatic Humidifier control button A ...

Страница 30: ...ution and or plate copy experiments The Experiment Designer tab is represented by an Erlenmeyer flask button In the screenshot below we have divided the experiment designer into various sections Each section is marked with a green number Sub sections of those major sections are represented by orange letters Use the table below to learn about each section and what it does 1 The Toolbar The Experime...

Страница 31: ...a task by double clicking it To delete a task click on the task and press the DELETE key on your keyboard or click the Delete button To change the order of execution for the items in the Task List select the desired task list and click the green up or down arrows to move the task up or down the list You can also right click on the desired task list and select Move Up or Move Down You can also chan...

Страница 32: ...elected here matches the plate type being used in your experiment New plate definitions that you create and save are automatically added to this library You can search for a plate type by typing the plate name in the Available Plate Types box 5 Status Bar The Status Bar is located at the bottom of the NT8 window It keeps you apprised of important operating parameters 6 Head Panel The Head Panel di...

Страница 33: ...e desired well column number by clicking on the corresponding number For multiple selection hold the SHIFT key and click the desired well column number If a column is selected it appears in dark blue The NT8 will perform the action on the selected number Liquid Class Provides four liquid class options Normal N Medium M High H and Very High V It s important to select the correct liquid class for ea...

Страница 34: ...r right click menu options Setting Up the High Volume Tip Caddy Plate Copy Option Only 1 Drag and drop the HV Tip Caddy container into the HV tip caddy station skip this step if the HV Tip Caddy container is already there 2 Right click on the HV tip caddy station and select Configure HV Tip Caddy The Tip Caddy Setup dialog box appears Area Description Information Add Adds the command s to the Task...

Страница 35: ...h Station and select Open Advanced Options The Task Details dialog appears 2 Set the wash time by typing a new value into the corresponding field or clicking the up or down arrows to increase or decrease the wash time Add Adds the specified settings to the Task List and closes the Task Details dialog Add More Adds the specified settings and add more commands as needed to the Task List Close Exits ...

Страница 36: ... increase or decrease the volume Liquid Class Enables you to select the protein liquid class THE PROTEIN SOURCE STATION The protein source station is an area in the software where you interact with your protein source Here you define the initial protein source volume and the protein liquid class These settings are needed for sitting drop hanging drop seeding and serial dilution experiments Prerequ...

Страница 37: ...o the Task List and closes the Task Details dialog Add More Adds the task to the task list and waits for you to add another aspirate or dispense command Close Exits the Task Details dialog Note The Aspirate Dispense option is enabled only if you have already added Pick Tip command to the Task List see The Tip Caddy Stations Use Custom Aspirate Displays the Aspirate Custom Height menu Note This opt...

Страница 38: ...r the high volume HV tip is attached to the HV tip attachments or not If the HV tip is attached the HV tip indicator button will turn green Low Volume Tip Indicator Indicates whether the low volume LV tip is attached to the LV tip attachments or not If the LV tip is attached the LV tip indicator button will turn green LCP Syringe Indicator Indicates whether the LCP syringe is attached or not If th...

Страница 39: ...STERBLOCK 96 Deep Well from the Plate Type Library into the deep well station The Deep Well Levels Setup dialog box appears as shown below There are 12 graphics that represent the well columns and each of them has 20 level numbers Level 1 is located at the bottom and level 20 is at the top 2 Set the well volume to aspirate by clicking the desired level numbers 1 through 20 on every column graphic ...

Страница 40: ...t Dispense right if you want the NT8 to perform dispense Black Arrow Specifies the direction of a specific Action Normal direction column 1 to column 12 is indicated by the right arrow Column Represents the well column numbers Select the desired well column number as needed by clicking on the corresponding number For multiple selection hold the SHIFT key and click the desired well column number Th...

Страница 41: ...Task List Close Exits the Task Details dialog Next to the Experiment Designer tab is the Rock Maker Integration tab This tab is only enabled if you use Rock Maker as your LIMS Here is where you can import and run an experiment as defined in Rock Maker For further information on how to create an experiment in Rock Maker please see the Experiment Design section of the Rock Maker help file The Basics...

Страница 42: ...periment properties display after the experiment s barcode is validated The next section explains this area in detail Rock Maker Experiment Properties Panel Rock Maker experiment information appears after your experiment barcode is validated The Rock Maker Experiment Properties Panel then has three different tabs A Instruction tab Instruction tab shows the detailed and required instructions that s...

Страница 43: ...Experiment N A Stock Name The stock name Type The experiment layer type as defined in Rock Maker Target The dispense target as defined in Rock Maker Liquid Class Shows you the liquid class of the protein and well solutions used Status Tells you the current stock status Item Configurable Description Barcode N A The experiment barcode number Name N A The experiment name as defined in Rock Maker Plat...

Страница 44: ...ion to the drop location then aspirate protein from the protein block and dispense it into the drop location This action will repeat column by column All Protein First then Well Use this option to instruct the NT8 to first aspirate protein and dispense it to every drop location on the plate then aspirate well solution and dispense it to all drop locations on the plate Protein then Well Use this op...

Страница 45: ...his property is only available for system with Plate Copy feature You can select whether you want to dispense screen by using the Plate Copy or not Status N A Tells you the current experiment status If you have run the dispensing process the status will be marked as Dispensed otherwise it will be blank ...

Страница 46: ...n the protein block and put the protein tray clamp on top of the protein tray to hold it in place You will pipette your protein into the protein block after you set up the software Option 1 Set up the Software with the Sitting Drop Template A Sitting Drop experiment setup template is pre installed with your software You may use it if you plan to combine 100 nL well solution with 100 nL protein for...

Страница 47: ... appropriate location in the software Drag and drop the LV Tip Caddy from the Plate Type Library to the LV Tip Caddy station Right click the protein block image and select Set Protein Volume Type in the volume of protein available in each well of each column in the tray that you plan to use 2 Add the Pick Tip command to the Task List by double clicking the LV Tip Caddy image 3 Click the Repeat but...

Страница 48: ...nt the NT8 to mix your solution enter the number of mixing cycles next to Mixing optional Select a Liquid Class from the liquid class list Next to Z Offset you may choose to let the NT8 s Flexible Fingers determine the dispense height recommended or manually specify the distance you want the fingers to stop at before dispensing Click the drop location in the Select Target area drop 1 is selected i...

Страница 49: ... Well Crystallization Plate We ve highlighted this step with a red text in the steps that follow Power On the NT8 and Start the Software Connect the NT8 to your computer using the USB cable Find the power switch located on the back right of the NT8 and turn it on Double click the NT8 icon on your computer s desktop It will take a few moments for the NT8 software to load while the NT8 initializes T...

Страница 50: ...trip clamp on top of the protein strip to hold it in place You will pipette your protein into the protein block after you set up the software Option 1 Set Up the NT8 Software Using the Hanging Drop Templates A hanging drop experiment template is preinstalled with your software You may use it if you plan to combine 100 nL protein with 100 nL well solution for a 200 nL dispense You must also use the...

Страница 51: ... the liquid class list Click Add 6 Instruct the NT8 to aspirate solution from the MRC 2 Well Crystallization plate Double click the plate image to open the Task Details dialog box Next to Action select Aspirate Next to Column click column 1 then press and hold the Shift key and click column 12 to select all columns Next to Volume enter the volume you wish to aspirate from the well Verify that the ...

Страница 52: ...ptional Save your Task List by clicking the Save button Add Protein to the Protein Block Using a hand pipette pipette the protein to be used in the hanging drop experiment into the protein block Run the Hanging Drop Dispense Click the Run Task List button to execute the Task List The NT8 head moves to the LV Tip Caddy picks up a column of tips then washes them at the wash station Next the NT8 will...

Страница 53: ...efully slide the reference plate to the right The adhesive surface on the UVP Seal should keep it in place while you remove the reference plate 4 Use a microplate seal roller to secure the UVP seal to the microplate surface to assure each well is properly sealed ...

Страница 54: ...r three plates follow the instructions under Option 2 Set Up the NT8 Hardware Place the HV tip caddy and deep well block on the NT8 Place the MRC 2 Well Crystallization plate on microplate station 2 Option 1 Set Up the NT8 Software Using the Plate Copy Template A plate copy template is preinstalled with your software It creates a task list that uses a MRC 2 Well Crystallization plate as the daught...

Страница 55: ...click column 12 to select all columns Next to Volume enter the volume you wish to aspirate Select a Liquid Class Click Add 5 Instruct the NT8 to dispense to the daughter plate Double click the microplate you want to dispense to open the Task Details dialog box Check that Dispense is selected next to Action Check that columns 1 12 are selected next to Column If selected they appear in dark blue If ...

Страница 56: ...ck the Run Task List button to execute the Task List The NT8 head moves to the HV Tip Caddy picks up a column of tips moves to the deep well and aspirates solution then moves to the daughter plate and dispenses solution Finally the NT8 head moves back to the HV Tip Caddy and disposes the used tips in the empty column then picks up new tips from the next column and repeats the process until your da...

Страница 57: ...ware Set up the NT8 software 1 Click The Mixing LCP Wizard dialog displays see below Number of mixing cycles Sets number of mixing cycles to be performed by the NT8 Mixing speed Sets the mixing speed to be performed by the NT8 2 Specify the settings on the LCP Mixing Wizard as needed Important The maximum total volume of the two syringes to be mixed should be 70 µL ...

Страница 58: ... of the reference hole located on the stage 3 Bring the mixer plunger to the hole by sliding it 4 Press down the locking knob until it gets into the hole and tighten the knob by turning it counterclockwise Please make sure it firmly locks the mixer plunger 5 Prepare two LCP glass syringes that have been filled with solution and protein 6 Mount the coupler on the LCP syringe and join the two LCP sy...

Страница 59: ... software click OK to confirm that you have connected the syringe to the mixer station The system mixes the syringe 2 After the mixing is finished a new dialog displays and notifies you to remove the LCP syringe from the stage 3 Push the plunger to the other syringe position Detach the conjoined syringes Attach the needle to the syringe that will be used in your experiment 4 Disconnect the mixer p...

Страница 60: ...e MRC 2 plate should contain about 50 µl 3 Prepare the LCP Plate Spray the LCP plate with a duster to remove any contaminants Peel off the brown packing tape Apply 2 3 drops of 70 Isopropanol to the reference plate surface Aligning the four corners drop locations to the crosshairs on the reference plate and affix the LCP plate to the reference plate 4 Attach the LCP syringe to the NT8 s head Using...

Страница 61: ...s You must also use the MRC 2 Well Crystallization plate as your well solution source To use this template 1 Click the Open Task List button 2 Open the Sample folder and select the LCP 50 nL Monolein And 800 nL Well Solution task list 3 Click Open 4 Run your experiment by clicking the Run Task List button Option 2 Manually Set Up the Software 1 Drag the LV Tip Caddy from the Plate Type library to ...

Страница 62: ...he Task Details menu In the Select Head image click the LCP head to select it The selected head is highlighted in yellow Next to Action select Dispense Next to Column click column 1 then press and hold the SHIFT key and click column 12 to select all columns Next to Stock Name select the name of your LCP sample Next to Volume select the amount of LCP sample you want to dispense to each drop from th...

Страница 63: ...button to execute the Task List The NT8 picks up the LV tips washes them aspirates from the microplate containing the well solution then dispenses the LCP sample across a column and then dispenses a column of well solution to the LCP sample drops This process repeats for each column you specified for the LCP plate After the NT8 has completed the tasks for each column it disposes the tips in the wa...

Страница 64: ...code number in the Experiment Barcode field at the upper left corner of your screen or scan the barcode with a barcode scanner if it is available When the barcode is validated the Rock Maker experiment information populates 3 Next follow the on screen instructions presented in the Instruction tab The on screen instructions tell you to do some hardware preparations For example you may be told to pl...

Страница 65: ...Maker Experiments with NT8 4 Tick all the checkboxes to confirm that you have completed all the required instructions as shown in the picture below 5 A notification message will appear Click Yes to continue then follow the on screen instructions ...

Страница 66: ...ach Distilled water LV tips To Clean the Liquid Lines 1 Bleach the water carboy Keeping the tubing attached unscrew the carboy s lid to open it Fill the carboy with distilled water to the maximum level marker Add enough bleach to the carboy to create a 5 bleach solution Mix well Screw the carboy s lid back on 2 Power on the NT8 and start the NT8 software Sterilizing the Liquid Lines Note In this c...

Страница 67: ...rcent Bleach Solution Sterilization Run Every Three Months and save it for future use 6 Sterilize the NT8 s liquid lines Click the Run Task List button to run the task list you created in step 5 When the NT8 finishes the task list wait for 5 seconds to let the pump rest in order to prevent overheating Repeat this process two additional times for a total of three wash cycles 7 Rinse the carboy to r...

Страница 68: ... months or as needed If you are noticing aspiration or dispense issues when trying to perform a plate copy also known as creating daughter plates from a deep well block you should perform the actions in this tutorial as a troubleshooting step The estimated time required to lubricate the plate copy plungers is 30 minutes Before you begin please make sure there are no Low Volume tips attached to the...

Страница 69: ...cover to expose the inner workings of the NT8 s head Lubricating the Plate Copy Plungers 1 Plug the NT8 into a power supply and power it on 2 Start the NT8 software and wait for it to connect to the NT8 hardware 3 Click the Programmatic Mode Tab 4 Go to the Control Manual Motion and click the UP arrow below to C by using a medium speed The plate copy plungers will move up exposing eight metal cyli...

Страница 70: ...e Sample folder 2 Select Plate Copy MRC2 50 uL xml and click Open The Task List will update 3 Run the task list by clicking the Run Task List button 4 Watch closely to verify that the Plate Copy head picks up the HV tips and that each of the tips aspirates 100 µL from the Deep Well block The amount aspirated into the tip should form an equal horizontal line across each of the tips Check again to m...

Страница 71: ... that create air tight seals around each of the eight nozzles If you are noticing an aspirate or dispense volume problem when attempting to copy a plate you should lubricate the O rings as part of a troubleshooting effort Otherwise the O rings should be lubricated every six months The estimated time required to grease the NT8 O ring is 3 minutes Before you begin please make sure that there are no ...

Страница 72: ...ightly but evenly apply grease to the front and back of each of the eight O rings on the NT8 s plate copy head 3 When you are finished power on the NT8 and continue with Running a Plate Copy Test If you are attempting to lubricate the plate copy plungers you will first need to edit the user interface configuration file in order to display the programmatic tab To enable the programmatic tab 1 If yo...

Страница 73: ...nce 4 Locate the following string and edit it so that it looks like this without the highlight add key UserInterface ProgrammaticTab Visible value true 5 Save the changes 6 Restart the software You should now see the Programmatic tab as shown below ...

Страница 74: ...command to the Task List by double clicking the LV tip caddy image Right click the wash station image and select Open Advanced Options Change the wash time to 20 seconds Click Add Click the Run Task List button to run the task list Try a test aspirate dispense after running the extended wash cycle If you are still aren t seeing results contact Formulatrix support If you are having difficulties wit...

Страница 75: ... the lines Please check your water source tank fill it if necessary and then prime the system by clicking the Start System Priming button If that doesn t fix the problem please contact support Q What is the minimum volume that the NT8 can reliably aspirate and dispense A Using the LV head the NT8 can reliably aspirate and dispense 100 nL with a CV average of 5 Q How much volume can the protein tra...

Страница 76: ...py or daughter plate creation experiments Protein Block A replacement part for the protein block that contains protein trays High Volume Protein Block A replacement part or optional accessory to support experiments that need more protein than a Low Volume Protein Block is designed to hold Reference Plate The reference plate is a clear plate that has crosshairs located at each corner The crosshairs...

Страница 77: ...PC Water Carboy The water carboy is the water reservoir for the NT8 It is used by the humidifiers and the wash station Waste Carboy The waste carboy is used for liquid waste Wash and Waste Tubes The wash and waste tubes connect to the wash and waste carboys and the wash and waste inputs at the back of the NT8 LV Head and LCP Head Calibration Pin The pin is used for calibrating the LV head and the ...

Страница 78: ...task list Pauses the running sequences This option displays only if the system is executing a task list Sends the device arm to its default home position and fixes its position when the motor is skidded or skipped Moves the NT8 s head to the waste station so that you can load and unload plates and tip caddies and otherwise interact with components on the rights side of the stage Moves the NT8 head...

Страница 79: ...eed Note This button displays only if you have the LCP attachment Calibrates the Lipidic Cubic Phase LCP syringe Note This button displays only if you have the LCP attachment Displays the settings and calibration operations Note Calibration operations should only be performed by Formulatrix support or advanced users Displays options to open the data directory open the config directory or open the ...

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