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Table 17: Program Settings with Staining Results 5 and 6

Program Setting Figure 5 Figure 6

Prewash Alcohol

Prewash Bluing

Nuclear/Htox

Acid Alcohol Wash

Bluing

Bluing Wash

Orange G

Orange G Wash

Cytoplasmic/EA

Final Wash

End Spin

Figure 5

Figure 6

Appendix D: Stain Modes and Programming Options

Содержание AEROSPRAY CYTOLOGY 7522

Страница 1: ...USER S MANUAL AEROSPRAY CYTOLOGY MODEL 7522 Slide Stainer Cytocentrifuge...

Страница 2: ...2 AEROSPRAY Cytology Slide Stainer Cytocentrifuge MODEL 7522 USER S MANUAL 57 0197 01A Last update 03 23 2020...

Страница 3: ...Group Inc Aerospray Cytopro are trademarks of ELITechGroup Inc Other trade names used in this manual are trademarks of their respective owners used here for information only ELITechGroup Inc makes no...

Страница 4: ...25 The Clean Cycle 27 Performing Tests 28 Reagent Level Monitoring 28 Section 3 Controlling and Customizing Stainer Functions 3 1 System Setup Menu 30 Accessing the System Setup Menu 30 Stain Programs...

Страница 5: ...58 Cleaning Broken Slides 58 Cleaning External Bottle 58 5 5 Decontaminating the Stainer and Carousels 59 Solutions for Decontaminating the Instrument 59 Figure 13 Lid Latch and Locking Pin Hole Locat...

Страница 6: ...Safety Phrases 82 Appendix C Accessories and Supplies 87 Appendix D Stain Modes and Programming Options 88 General Settings 89 Intensity Stain Settings 90 Wash Settings 91 Appendix E Technical Backgr...

Страница 7: ...wn in the following examples WARNING A Warning alerts to the possibility of personal injury death or other serious adverse reactions stemming from the use or misuse of this instrument or its component...

Страница 8: ...serious instrument damage always use reagents supplied by ELITechGroup Using reagents not supplied by ELITechGroup may void the warranty CAUTION Only spare parts supplied or specified by ELITechGroup...

Страница 9: ...trifugation before staining Table 1 General Specifications Category Characteristics Slide Carousel Capacity 1 to 12 or 1 to 30 depending on carousel Carousel Rotation Speed Reagent application 10 rpm...

Страница 10: ...and DF hematoxylin Reagents NOTE Use only ELITechGroup reagents REF numbers for this stainer include the following SS 051A SS 051B SS 051C SS 051D SS 051D2 SS 051E and SS 051F See Appendix A for detai...

Страница 11: ...ailed instructions and warnings Table 4 Approximate Stain Consumption mL The following table provides an estimate of the volume of stain used per staining cycle when running a full 12 slide carousel w...

Страница 12: ...Symbol for Recovery Recyclable In vitro Diagnostic Device In vitro Diagnostic Medical Device Keep away from sunlight Keep away from heat Manufacturer Waste Electrical and Electronic Equipment Reagent...

Страница 13: ...n 1 Introduction Symbol Description Corrosive Environment Hazard Flammable Harmful Irritant Oxidant Toxic Product to be used for manual cleaning only Do not pump product through instrument Systemic he...

Страница 14: ...l 2 12 Slide Carousel 3 Lid with Safety Lock 4 Bowl 5 Front Panel with Touch Screen Display 6 Right Side Panel with Label Indicating Reagent Positions A EA 50 Stain B Orange G Stain C Bluing Agent D H...

Страница 15: ...ction 1 3 Table 7 for more information Figure 3 Rear Panel 1 Level Detect Connection for Reagent F Acid Alcohol Wash 2 USB Ports 3 Network Ethernet Connection 4 Exhaust Vent 5 Rear Panel Label 6 Bioha...

Страница 16: ...Bluing Agent E Alcohol Wash 3 Drive Hub Figure 5 Nozzle Diagram 1 Mixing Insert 2 Compression Screw 3 Swirl Cone 4 Nozzle Housing Table 6 Preventive Maintenance Kit Catalog Number AC 184 Component Na...

Страница 17: ...r bowl Nozzle Wrench Disassembles the nozzle Nozzle Brush Cleans nozzles without removing them from the stainer Volume Test Collection Tubes small tube Collects reagents while performing the Volume Te...

Страница 18: ...d Items The following parts are not available worldwide from ELITechGroup but they can be obtained locally Approved alcohol Ethanol or Isopropanol Empty bottles are available from ELITechGroup CAUTION...

Страница 19: ...Performs the selected Clean Cycle System Clean left button Carousel Clean right button Cyto Enters the Cytocentrifuge mode System Information Shows the system information including serial number and s...

Страница 20: ...tect Allows users to manage the automatic reagent level detection system Language Allows users to change the display language System Log Allows users to control logging functions Network Settings Allo...

Страница 21: ...s a selected or enabled option Table 9 Maintenance Function Keys Button Name Description Individual Prime Buttons A B C D E F Primes the selected line ABCDEF Prime button Primes all A B C D E F reagen...

Страница 22: ...nts of the boxes match the packing lists for the instrument and accessories 3 Open the instrument lid and remove the cardboard tube that protects the hub NOTE Keep the box and packaging material to re...

Страница 23: ...the waste monitoring cable to the rear panel receptacle 5 Connect the waste monitoring cable to the waste container lid Connecting Power NOTE Use a surge protector to isolate the instrument from elect...

Страница 24: ...organic solvents in this instrument unless supplied by ELITechGroup or specified in official ELITechGroup formulation instructions NOTE Immediately remove spills in the reagent tray to preserve the a...

Страница 25: ...for instructions on using the barcode reader Preparing the Stainer for Operation Priming Procedures NOTE The instrument is shipped with alcohol in the reagent lines For proper performance this alcoho...

Страница 26: ...each reagent line with 200 mL of reagent to remove all the alcohol from the reagent lines and pumps Requires pressing the 60 Sec Prime button at least 2 3 times to prime each line sufficiently 8 Choos...

Страница 27: ...UTION Never place any carousel loaded with specimens in the instrument for a Clean Cycle including placing the instrument in standby mode Specimens will be damaged if they contact reagents sprayed fro...

Страница 28: ...when the reagent level is getting low The instrument will continue running through these warnings The user must monitor and replenish the reagent before running a stain cycle Enabling Disabling Reage...

Страница 29: ...Press Zero The display shows 5 Remove all reagent bottles and press Start The display shows NOTE Vibrations or bumps to the instrument or lab bench can cause inaccuracies in zeroing or calibration 6 A...

Страница 30: ...s Setting the date and time Restoring default settings Accessing the System Setup Menu 1 Press System Information from the Main menu 2 Press System Setup Stain Programs Stain Programs allows the user...

Страница 31: ...ased to remain within the 12 program limit 2 Press Add 3 Select the desired Stain Mode Progressive or Regressive and Nuclear Htox and Cytoplasmic EA Intensity then press Continue NOTE Staining program...

Страница 32: ...ame button and enter the desired name using the keypad 8 Press Enter on the keypad 9 Press Save Editing Renaming or Adjusting Stain Programs 1 From Stain Programs menu select the program to be modifie...

Страница 33: ...dministrator account and multiple up to 50 user accounts can be created The Administrator controls access to the system by adding and editing user accounts Users cannot edit System Settings unless per...

Страница 34: ...e Global Login 5 Select Add User 6 Enter a new user name 7 Press Enter 8 Enter a numeric passcode at least 4 numbers for the user account 9 Press Enter 10 Re enter the passcode to confirm 11 Press Ent...

Страница 35: ...automatically logging the user out 2 Press Login 3 Enter the correct passcode for the selected user and press Enter 4 Control returns to the Main screen and the stainer is ready for programming and s...

Страница 36: ...onitoring can be turned On and Off from the System Setup screen The system defaults to On Tray and External for reagent monitoring and to Off for waste container monitoring See Section 2 2 for complet...

Страница 37: ...Log The instrument records all login logout stain or cytocentrifuge cycles setting changes maintenance functions and specimen identification if enabled Accessing Logs 1 From System Setup press System...

Страница 38: ...ollowing QC Maintenance Tracking options are disabled Enable Stain Slide Tracking Enable Cyto Slide Tracking Manual Entry Enable Preventive Maintenance Tracking Enable Reagent Tracking Enable Stain Sl...

Страница 39: ...imen Information Section 3 2 6 See Section 4 for remaining steps for running a stain cycle Enable Cyto Slide Tracking Allows slide tracking in cytocentrifuge mode See the Cytopro Rotor Applications Ma...

Страница 40: ...C D E F Restoring Software Defaults 1 From System Setup select Restore Defaults CAUTION Restoring the system defaults will remove all personal settings Restoring System Settings will delete all usern...

Страница 41: ...Slides See Scanning Slides with the Barcode Reader 3 Press Back twice to return to the Main menu 4 Press Load Slides on the Main menu The Scan and Load Slides menu will appear 5 Scan the barcode of e...

Страница 42: ...desired Reagent A B C D E F 6 Scan the barcode of each enabled reagent bottle 7 Press Save 8 Verify that the barcode appears on the Reagent Information menu 9 Repeat steps 4 8 for each reagent bottle...

Страница 43: ...and run stain cycle as shown in Section 4 1 Manually Entering Reagent Information 1 Press Reagents from the System Setup menu or press the reagent status icon on the Main menu to reveal the Reagent In...

Страница 44: ...ides Correct Reagents and Locations Selecting a Staining Program System Setup Setting up Stain Programs System Setup Help Setting up Cyto Programs Setting up Users Setting up Level Monitoring System S...

Страница 45: ...ion 3 Controlling and Customizing Stainer Functions Using Help 1 Press Help to access the help function 2 Select the desired topic 3 Use the direction arrows to navigate 4 Press Exit to return to the...

Страница 46: ...ides on the Main menu Perform a stain cycle Unload the carousel Performing a Hub Pattern Test Use the Hub Pattern Test to ensure the nozzles are clear of debris and spraying properly 1 From the Mainte...

Страница 47: ...sel lid by pressing the button and lifting the lid 3 If Slide Tracking is enabled press Load Slides If using the barcode reader scan each specimen slide barcode before loading it into the carousel Sli...

Страница 48: ...and locked Using Blocking Slides If the carousel is not full blank slides should be used as blocking slides Blocking slides prevent overspray of reagents onto the specimen slides Overspray can cause...

Страница 49: ...reader or keypad the number of slides is programmed automatically Adjust the total number of slides if adding other specimen slides that have not been entered by barcode reader or keypad NOTE Do not i...

Страница 50: ...if enabled and by direct inspection of the bottles The monitor will show the approximate level of each reagent This can be compared to the actual level in the bottles Never allow a reagent to run dry...

Страница 51: ...ion 3 1 6 Unscrew the cap and remove the dip tube from the empty bottle 7 Insert the dip tube into the new reagent bottle and screw on the cap 8 Place the new bottle in the tray Adding Reagent F to th...

Страница 52: ...te container should be emptied It is still necessary to check waste levels visually to ensure the waste container does not overfill CAUTION Dispose of collected waste according to local statutes and s...

Страница 53: ...n the exterior of the instrument with 70 to 100 alcohol or SS 029 nozzle cleaning solution Wipe clean with a paper towel 6 Ensure the maintenance procedures listed on the Maintenance Log have been per...

Страница 54: ...Section 6 5 Using the Preventive Maintenance Log With Preventive Maintenance Tracking enabled the Preventive Maintenance Log provides a convenient and structured means of recording important maintena...

Страница 55: ...55 55 Section 5 Preventative Maintenance and Safety 3 Press Save to record entries...

Страница 56: ...crew the cap and remove the dip tube from the reagent bottles 3 Place the end of the dip tube in a bottle of 50 DI water 50 methanol 4 Flush at least 250 mL of 50 DI water 50 methanol through each rea...

Страница 57: ...ower OFF the instrument 2 Disconnect the power cord from the power outlet and the rear panel of the instrument 3 Open the fuse cover by inserting a screwdriver in the slot on the right side of the cov...

Страница 58: ...ting the Stainer and Carousels below for instructions Cleaning Broken Slides Take stringent precautions if a slide breaks inside the instrument during a staining cycle especially if the instrument has...

Страница 59: ...is very important to thoroughly decontaminate the instrument before removing it from the laboratory or before performing any technical service This procedure may not be effective against prions WARNIN...

Страница 60: ...h excessive moisture Any moisture that seeps through could damage the internal electronics 8 Repeat the spray treatment of exterior surfaces every 2 or 3 minutes for a total of 20 minutes Do not allow...

Страница 61: ...be charged to the user CAUTION Ship the instrument or carousels in containers comparable to the original packaging Hazard Free Certification The operator must print and complete the Hazard Free Certif...

Страница 62: ...rew cannot be easily loosened use light penetrating oil and a 5 8 in wrench to loosen the nozzle 3 Place the nozzle parts in a 50 mL conical tube that has been clearly marked with the correct nozzle p...

Страница 63: ...cone lubricant to the compression screw threads 7 Reassemble the nozzle by placing the compression screw on the nozzle disassembly tool then inserting the swirl cone into the compression screw NOTE Ho...

Страница 64: ...he Main menu 7 Press Volume Test 8 Press the desired prime button to start the reagent pump 9 Run the reagent into the tube until the fluid is free of bubbles then press Stop WARNING Do not pull the p...

Страница 65: ...nozzle 8 Compare collected nozzle volumes with the following table Table 11 Volume Test Tolerances Nozzle Reagent Line Minimum Maximum A 9 0 mL 11 0 mL B 9 0 mL 11 0 mL C 9 0 mL 11 0 mL D 7 5 mL 10 0...

Страница 66: ...repare the Maintenance Kit for future use Empty the contents of the tubes into the stainer bowl Rinse the tubes with water Place the tubes back into their original place in the Maintenance Kit or tube...

Страница 67: ...s that is being flushed 4 Insert an empty carousel and close the lid 5 Press Start The instrument pumps approximately 400 mL of water through the line s A status bar indicates progress and automatica...

Страница 68: ...tions 1 and 2 2 Load the carousel into the stainer and close the lid 3 From the Main menu press Maintenance 4 Press Pattern Test 5 Press the corresponding button for the reagent line to be tested 6 Re...

Страница 69: ...r is On and you press the prime button Follow the procedures in Section 6 3 for priming reagent pumps A reagent line will not prime even with the priming tool Section 6 3 Press the priming button and...

Страница 70: ...otor or electronic component malfunctions require servicing of internal components Contact your dealer or ELITechGroup for assistance If the display shows Rotor Imbalance make certain the Cytopro roto...

Страница 71: ...load load the slides in the correct positions as indicated by the markings on the carousel see Section 4 1 Review sample and slide preparation procedures in Appendix E Abnormal staining on entire surf...

Страница 72: ...ge G molecule is considerably smaller than the eosin y or fast green molecules and can therefore permeate the dense cell membranes more easily and quickly If a specimen has not been fixed and then air...

Страница 73: ...line with at least 100 mL Remove coverslip and mounting media from the slide using xylene or xylene substitute and dehydrate in 100 ethanol then recoverslip In areas of high humidity dip slides in Rea...

Страница 74: ...install a computer type surge protector to isolate the instrument If possible connect the stainer to a power circuit not shared by centrifuges refrigerators air conditioners or other motorized equipme...

Страница 75: ...s have been verified the instrument may require service A reagent line leak between the pump outlet and the nozzle will cause fluid to leak into the interior of the stainer housing and ultimately onto...

Страница 76: ...an error message NOTE While zeroing or calibrating do not bump the instrument or lab bench Ensure that no nearby equipment vibrations can be transmitted to the stainer Calibrating the Reagent Level D...

Страница 77: ...es to the tray as indicated in Section 2 1 to prepare for staining NOTE For accurate reagent level detection and calibration dip tubes must follow their pre formed coiled shapes Calibrating the Touchs...

Страница 78: ...cal service and support ELITechGroup Inc 370 West 1700 South Logan Utah 84321 8212 USA Telephone 800 453 2725 United States Canada 1 435 752 6011 International calls Fax 1 435 752 4127 US 1 435 752 41...

Страница 79: ...Reusable or disposable chambers single and dual Holds eight slides and chambers User programmable memory locations for settings speed acceleration rate and time Easy switching between staining and cyt...

Страница 80: ...e to the low concentration of this basic solution Orange G Stain The Orange G stain solution SS 051B is specifically formulated for staining with the pre programmed staining times of the Cytology stai...

Страница 81: ...ytology Hematoxylin I Stain 20 35 Ethylene Glycol 1 5 Aluminum Sulfate Hydrate 1 2 Acetic Acid SS 051D2 Aerospray Cytology Hematoxylin II Stain 20 35 Ethylene Glycol 1 5 Aluminum Sulfate Hydrate 1 5 A...

Страница 82: ...ir Take off immediately all contaminated clothing Rinse skin with water shower P305 P351 P338 If in eyes Rinse cautiously with water for several minutes Remove contact lenses if present and easy to do...

Страница 83: ...luing Agent Hazard Pictogram Single word Warning Hazard statements H226 Flammable liquid and vapor Precautionary Statements P210 Keep away from sparks open flames No smoking P260 Do not breathe vapors...

Страница 84: ...ments P264 Wash hands thoroughly after handling P270 Do not eat drink or smoke when using this product P280 Wear protective gloves eye protection Refer to SDS for additional Precautionary Statements S...

Страница 85: ...inse skin with water P304 P340 IF INHALED Remove person to fresh air and keep comfortable for breathing P305 P351 P338 If in eyes Rinse cautiously with water for several minutes Remove contact lenses...

Страница 86: ...g vapors mist or spray P264 Wash hands and other exposed areas thoroughly after handling P280 Wear protective gloves protective clothing and eye protection P302 P352 If on skin Wash with plenty of wat...

Страница 87: ...olution Concentrate 250 mL Dilutes to 500 mL SS 029C EA 50 Stain 500 mL bottle SS 051A Orange G Stain 500 mL bottle SS 051B Bluing Agent 500 mL bottle SS 051C Hematoxylin I 500 mL bottle SS 051D Hemat...

Страница 88: ...luing 0 0 0 0 0 0 Nuclear Htox 1 5 9 1 5 9 Acid Alcohol Wash 0 1 3 1 5 7 Bluing 1 2 3 1 2 3 Bluing Wash 2 3 5 2 4 7 Orange G 1 5 9 1 5 9 Orange G Wash 2 3 5 2 5 8 Cytoplasmic EA 1 5 9 1 5 9 Final Wash...

Страница 89: ...applications to remove the carbowax fixative Stain Setting 1 2 3 4 5 6 7 8 9 Spray Applications 3 3 4 4 4 5 5 7 9 Wait Applications 0 2 0 2 3 0 3 6 9 Prewash Bluing This setting adds a series of bluin...

Страница 90: ...31 39 Orange G Increase decrease numbers to strengthen reduce orange color of keratinized cells While automatically set by the cytoplasmic intensity it can be varied independently Stain Setting 1 2 3...

Страница 91: ...actory results Stain Setting 1 2 3 4 5 6 7 8 9 Spray Applications 2 3 4 5 6 7 8 9 10 Wait Applications 0 0 0 0 0 0 0 0 0 Orange G Wash Removes excess OG reagent in preparation for application of EA re...

Страница 92: ...plications 0 0 0 0 0 0 0 0 0 Orange G Spray Applications 4 5 6 7 9 11 15 20 25 Wait Applications 6 9 12 15 24 29 39 54 72 Orange G Wash Spray Applications 2 3 4 5 6 7 8 9 10 Wait Applications 0 0 0 0...

Страница 93: ...d and then cover slipped and viewed at a magnification of 10x Table 15 Program Settings with Staining Results 1 and 2 Program Setting Figure 1 Figure 2 Prewash Alcohol 1 1 Prewash Bluing 0 0 Nuclear H...

Страница 94: ...ting Figure 3 Figure 4 Prewash Alcohol 1 1 Prewash Bluing 0 0 Nuclear Htox 3 7 Acid Alcohol Wash 3 3 Bluing 1 3 Bluing Wash 2 4 Orange G 3 7 Orange G Wash 2 4 Cytoplasmic EA 3 7 Final Wash 2 6 End Spi...

Страница 95: ...ting Figure 5 Figure 6 Prewash Alcohol 1 1 Prewash Bluing 0 0 Nuclear Htox 9 9 Acid Alcohol Wash 3 9 Bluing 3 3 Bluing Wash 5 5 Orange G 9 9 Orange G Wash 5 5 Cytoplasmic EA 9 9 Final Wash 6 6 End Spi...

Страница 96: ...Use one of the following methods of fixation Use spray fixative and allow to air dry for a minimum of 15 minutes Immerse in 95 alcohol for up to 30 minutes but no less than 10 minutes Immerse in a Sac...

Страница 97: ...lene glycol carbowax Carbowax protects cells from air drying before fixation but can inhibit staining if not completely removed Because the aqueous hematoxylin reagent used in the ELITechGroup stainin...

Страница 98: ...esumably by altering electrostatic interactions In addition a mordanting effect of phosphotungstic acid for light green has been described 6 7 and other mechanisms may also be involved 4 The cytoplasm...

Страница 99: ...rn commercial stain It recognizes Baker s observation that hematein is much more soluble in ethylene glycol than water 20 It employs half oxidized hematoxylin according to Baker and Jordan 21 and the...

Страница 100: ...s can be modified as described by Boon and Drijver 5 6 Raising the pH enhances the blue color and lowering the pH shifts the color to green 6 Acid Alcohol Wash The Acid Alcohol Wash is designed to cle...

Страница 101: ...T Wollenberg and G F Guenther American Journal of Clinical Pathology 83 1 132 133 1985 17 E K W Schulte and D H Wittekind Standardized ThioninEosin Y A Quick Stain for Cytology Stain Technology 64 5 2...

Страница 102: ...www elitechgroup com info elitechgroup com ELITechGroup Inc 370West 1700 South Logan Utah 84321 8212 800 435 2725 1 435 752 6011 REF 57 0197 01A...

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