90
Intensity Stain Settings
Nuclear (Hematoxylin)
The greater the numeric value selected, the more intense the application. Low settings require less time
than higher settings. These settings allow you to vary the staining results according to specimen thickness,
type of cells to be stained, or special situations. Refer to Section 13 Appendix E: Sample/Slide Preparation
Guidelines for sample treatment guidelines.
The higher the number the darker the stain, the longer the cycle and the more reagent used.
Stain Setting
1
2
3
4
5
6
7
8
9
Spray Applications
5
5
6
8
9
10
12
16
20
Wait Applications
6
7
9
12
22
26
35
51
67
Bluing
Increase numbers to better blue the hematoxylin in the nucleus. To conserve reagent, select minimum
value which produces satisfactory results.
Stain Setting
1
2
3
4
5
6
7
8
9
Spray Applications
6
7
8
9
11
13
17
19
23
Wait Applications
2
3
7
11
15
19
27
31
39
Orange G
Increase/decrease numbers to strengthen/reduce orange color of keratinized cells. While automatically set
by the cytoplasmic intensity, it can be varied independently.
Stain Setting
1
2
3
4
5
6
7
8
9
Spray Applications
4
5
6
7
9
11
15
20
25
Wait Applications
6
9
12
15
24
29
39
54
72
Cytoplasmic (Orange G and EA)
The greater the numeric value selected, the more intense (more stain applied over a longer period) the
application. Low settings require less time than higher settings. These settings allow varying the staining
results according to specimen thickness, type of cells to be stained, or special situations. Refer to Appendix
E for sample treatment guidelines.
NOTE:
If restaining coverslipped slides, the coverslipping and mounting media must first be removed by
soaking in xylene or xylene substitute.
The higher the number the darker the stain, the longer the cycle and the more reagent used.
Stain Setting
1
2
3
4
5
6
7
8
9
Spray Applications
4
6
7
8
10
12
16
21
26
Wait Applications
4
12
15
18
24
30
42
57
72
Appendix D: Stain Modes and Programming Options
