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26
Section 2: Setup and Preparation for Operation
4. Press
Maintenance
from the Main menu.
5. Press the
A
prime button. Stain should appear within 10 seconds. When
properly primed, a steady stream of reagent (no sputtering or breaks) flows
from the nozzle receptacle.
◊
If stain appears, proceed to the next step.
◊
If stain does not appear within 10 seconds, perform the manual priming
procedure in Section 6.3.
CAUTION:
Never operate a dry pump for more than 10 seconds. Operating a dry pump may cause damage to
the instrument.
6. Repeat the previous steps for each reagent, (B, C, D, E and F).
7. Press
60-Sec Prime
to prime each reagent line with 200 mL of reagent
to remove all the alcohol from the reagent lines and pumps. (Requires
pressing the
60-Sec Prime
button at least 2-3 times to prime each line
sufficiently.)
8. Choose one of the following:
◊
For initial setup, press
ABCDEF
to prime all lines simultaneously.
(Requires pressing the
ABCDEF
prime button at least 2-3 times to
prime each line sufficiently.)
◊
To prime individual lines, press the appropriate individual prime button
(A, B, C, D, E, F). The pumps will run for 60 seconds and prime the
selected lines.
9. Return the nozzles to their original positions and tighten clockwise with the
nozzle tool.
10.
With the nozzles installed, repeat Steps 5 and 6. A fine cone of spray
should come from each nozzle.
11. After verifying nozzle performance, run the Clean Cycle (see below).
A B C
D
E
F