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Section 2: Setup and Preparation for Operation
Installing Standard 500 mL Reagent Bottles for the First Time
WARNING!
Reagents used in the instrument contain moderately hazardous chemicals that require care in
handling. Always handle reagents using appropriate safety measures, including gloves and eye
protection.
NOTE:
Reagents should be stored according to the conditions specified on their label. After opening,
reagents are stable until the expiration date specified on the label unless otherwise indicated.
1. Place each 500 mL reagent bottle in the correct position.
A. SS-051A – EA-50 Stain
B. SS-051B – Orange G Stain
C. SS-051C – Bluing Agent
D. SS-051D or SS-051D2 – Hematoxylin Stain
E. SS-051E – Alcohol Wash
F.
SS-051F – Acid Alcohol Wash (off tray)
NOTE:
See Appendix A for complete identification of all reagents used in this stainer.
CAUTION:
To avoid severe damage, never use reagents containing organic solvents in this instrument, unless
supplied by ELITechGroup, or specified in official ELITechGroup formulation instructions.
NOTE:
Immediately remove spills in the reagent tray to preserve the accuracy of the reagent level
detecting system.
7. For all reagents using the standard 500 mL bottles:
◊
Open a new bottle of reagent.
◊
Record the reagent letter on each cap and retain for future use (such as long-term storage).
◊
Remove caps from bottom of the dip tube, insert the corresponding dip tube and bottle adapter into
the reagent bottle, install and tighten the ring cap.
8. For reagents F:
◊
Open a new 500 mL bottle of reagent SS-051F.
◊
Pour the contents from the 500 mL SS-051F bottle into the external reagent bottle with level detect
(AC-197).
◊
Insert the level detect sensor into the reagent bottle and tighten the bottle cap.
◊ From the stainer side panel, connect the reagent F tubing to the barb fitting on the top of the external
bottle.
◊
Connect the level detect cable to the external bottle and to the connector on the back of the
instrument towards the side with the reagent tray.
A B C D E
F
