Dionex UltiMate 3000, Установка системы и приложений

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UltiMate 3000 Nano and Cap- System Installation and Application
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3.4 2D LC Salt Plug
This application supports online two-dimensional separation of peptides: While the
autosampler's injection valve is in
Load position, sample is drawn by the syringe through the
needle and placed in the sample loop. The needle pair withdraws liquid from the well or vial
and then descends into the wash port. The injection valve is switched to the
Inject position,
allowing the loading pump to direct the sample onto the ion exchange column (SCX). The
right valve in the flow manager is in position
10-1 . The part of the sample that is not retained
is trapped on the reverse phase trap column; the left valve in the flow manager is in position
10-1 . (For information about how to install the trap column, refer to section 3.7.)
When loading is finished, the ion exchange column is removed from the fluidic path by switching
the right valve to position
1-2 . This allows desalting the sample collected on the trap column with
the loading pump.
After the desalting step, the left valve is switched to position 1-2 to run the analytical gradient
through the trap column and the reverse phase separation column.
When the first fraction is separated, both valves are switched to position 10-1 and a salt solution
is injected. A new fraction of peptides elutes from the ion exchange column to the trap column.
The desalting and analytical steps described above are carried out.
The process is iterated with increasing concentrations of salt injected until all the peptides from
the ion exchange column are eluted and separated on the reverse phase column.
Dionex recommends that you stack the system modules as shown below:
Fig. 14: Module arrangement for 2D LC salt plug
SRD-3600 Solvent Rack
DGP-3600 Pump
VWD-3x00 Detector
FLM-3100 Flow Manager
WPS-3000 or WPS-3000T Autosampler