Covaris
LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
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Part Number: 010398 Rev H | November 2019
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If possible, please independently verify your analysis equipment. Covaris has DNA shearing verification kits available. Please contact
Covaris applications support or your local representative for assistance.
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SonoLab reported Power Level is lower or higher than expected:
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Verify that the average power values reported by SonoLab are approximately correct. The typical average power is a few percent below
the value “Peak Incident Power” multiplied by “Duty Factor”. For example, if PIP=200 Watts and Duty Factor is 10%, then an expected
power reading would be approximately 15 to 17 Watts.
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Contact Covaris or your local representative for assistance.
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Degas Pump problems:
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Verify that the water is clear and free of algae or other particulates. Perform a cleaning protocol with 10% bleach or equivalent
solution if this has not recently been done.
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Verify that the degassing pump is operating (observe the pump outlet on the left side of the tank – when the pump is running there should
be occasional bubbles exiting the pump outlet tube). Also examine the inlet “dip tube” visible from the right side of the tank. The inlet
holes (very small drilled holes in the side of the tube) should be free of obstruction.
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If the degas pump makes a very loud noise when operating, it may need to be replaced.
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Particulates in bath water
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Change the bath water with fresh DI water
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Perform a cleaning protocol with a 10% bleach or equivalent solution if this has not recently been done.
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Tube Breakage
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Verify that the acoustic parameters being employed are correct.
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Verify that average power levels are approximately correct (see Power Level).
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RF Board Limits Exceeded Error:
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If Peak Incident Power is set to values above 400 Watts, intense acoustic reflections may cause an overload in the electronic circuits
driving the transducer. To protect the drive circuits and transducer, the system may shut down and post a message, “RF Board Limits
Exceeded RF Error is Present”. Should this error appear, please retry your application with the following modifications:
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Fill the sample tube to eliminate splashing and large bubble formation in the sample
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Replace the existing bath water with new, clean bath water
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Lower the PIP (Peak Incident Power) – this is often more effective than applying very high power.
Repeat the method to verify that overload problems have been mitigated. If not, please contact Covaris or your local
representative.
Technical Support
– Ongoing assistance with the operation or application of the equipment and/or troubleshooting is provided via:
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Telephone
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United States: Tel: +1 781.932.3959 during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday, Eastern
Standard Time (EST), Greenwich Mean Time (GMT-05:00)
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Europe: Tel: 44 (0) 845 872 0100, during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday, Greenwich Mean Time
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E-mail queries to
