Covaris
LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
43
Part Number: 010398 Rev H | November 2019
7.
Maintenance
7.1
Cooling Air Intake
Periodically, unplug the unit and remove any obstruction to the fans and air vents on the rear panel to ensure that objects are not
restricting the flow of air.
7.2
Water Bath
Periodically, unplug the unit and remove any obstruction to the fans and air vents on the rear panel to ensure that objects are not
restricting the flow of air.
7.2.1
Recommendations for Daily Maintenance
Only distilled or deionized water should be used to fill the water bath. To avoid algae growth, the water bath should be emptied,
the degas lines purged, and the tank dried prior to longer term (more than a week) idle time. See ‘Shutdown for Long Term
Storage below.
7.2.2
Recommendations for Monthly Maintenance
Periodically, the water bath and degassing lines should be rinsed with a 10% solution of bleach (or equivalent, such as NaDCC).
The resultant sodium hypochlorite solution is ~ 0.5% NaOCl. Perform the following cleaning protocol:
1)
Turn off the WCS and CH05 chiller and disconnect the hoses from the front of the water tank.
2)
Start SonoLab and open the ‘Motion Control’ pane.
3)
Fill the tank with approximately 450 ml of household bleach or equivalent and the rest with DI water.
4)
Click the ‘Home’ button. The transducer will lower into the bath and the degas pump starts.
5)
Operate the degas pump for approximately 5 minutes.
6)
Click the ‘Service’ button. The transducer rises out of the bath and the degas pump stops.
7)
Empty the bleach/water solution.
8)
Place the empty water tank back into the instrument and click the ‘Home’ button. The transducer lowers into the bath and the
degas pump comes on purging the degas lines.
9)
Click ‘Service’. The transducer rises out of the bath.
10)
Empty the tank, rinse, and fill with fresh DI water.
11)
Place the water tank back into the LE220.
12)
Click ‘Home’. The transducer lowers into the bath and the degas pump starts. Allow pump to operate for 3 or 4
minutes.
13)
Click ‘Service’ to raise transducer out of bath and turn off pump.
14)
Repeat steps 10 through 12 twice more.
15)
Fill the tank with clean DI water, reconnect and power on the WCS and CH05 chiller. Allow the water to degas for an hour
prior to treating samples.
