Endosafe
®
- Portable Test System User’s Guide
Copyright 2003
Page C1
PIPTS101-10
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Endosafe
®
- PTS Inhibition/Enhancement Screen
PERFORMING INHIBITION/ENHANCEMENT TEST
An Inhibition/Enhancement PTS LAL assay is conducted by following the simple prompts
on the PTS instrument. Different dilutions of one (1) sample can be placed in each
sample well to determine the non-interfering dilution or different samples can be placed
in each well of one (1) test cartridge. The following represents a typical assay
procedure:
1.
Instrument Operation
x
Press
the
MENU
key on the PTS keypad to turn instrument on (Menu 5 turns
instrument off).
x
The PTS Reader initiates a “SYSTEM SELF TEST” as it heats up to 37°C –
this takes approximately 5 minutes.
x
The PTS Reader displays “SELF TEST OK” and then “INSERT
CARTRIDGE”.
2.
Insert the Cartridge
Remove cartridge from pouch and insert with sample
reservoirs facing up into slot at front of the PTS
Reader.
Press cartridge firmly into slot.
Note: Allow the cartridge to come to room temperature
in pouch before use.
3.
Enter Required Information
Once the cartridge has been firmly inserted into the PTS Reader, the PTS
Reader prompts the user to enter the following information:
x
Enter OID
(Operator ID)
x
Enter Lot #
(Cartridge Lot #)
x
Enter Calibration Code
(If the Calibration Code for the particular lot # has
already been entered, the reader does not prompt for the code again. (To
Erase all stored lot #’s and corresponding calibration codes, select menu, 2,
then 4 from the initial menu)
x
Lot #
(Confirms cartridge lot number entered)
x
Enter Sample 1 Lot #
(Sample 1 is designated as the well on the far left after
cartridge is inserted into the PTS Reader)
