Endosafe
®
- Portable Test System User’s Guide
Copyright 2003
Page B1
PIPTS101-10
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Endosafe
®
- PTS Rapid Micro Methods
Gram ID
PERFORMING ROUTINE GRAM ID TEST
A routine Gram ID assay is conducted by preparing a bacterial suspension and following
the simple prompts on the PTS Instrument. The following represents a typical assay
procedure:
The PTS Gram ID offers two (2) assay report formats. One assay result format will
report the presence of either Gram-negative microorganisms,
Yeast/Mold or Gram-positive microorganisms in approximately 7
minutes. The second assay result format will report the presence
of Gram-negative and Gram-positive in approximately 3 minutes
(this format does not differentiate between Gram-positive and
Yeast/Mold isolates). Reference the Certificate of Analysis for the
calibration code to be used for each assay result format.
Sample Preparation:
From an isolated culture, carefully remove colonies from agar
surface and suspend cells in approximately 2 mLs of LPS-free
Saline or LRW and adjust to a 0.5 McFarland Equivalence
Turbidity Standard.
Note: Do not use cotton tip swabs of any kind during sample
preparation.
1.
Instrument Operation
x
Press
the
MENU
key on the PTS keypad to turn instrument on (Menu 5
turns instrument off).
x
The PTS Reader initiates a “SYSTEM SELF
TEST” as it heats up to 37°C – this takes
approximately 5 minutes.
x
The PTS Reader displays “SELF TEST OK” and
the “INSERT CARTRIDGE”.
2.
Insert the Cartridge
Remove cartridge from pouch and insert with sample
reservoirs facing into slot at front of the PTS Reader.
Press cartridge firmly into slot.
