Endosafe
®
- Portable Test System User’s Guide
Copyright 2003
Page B2
PIPTS101-10
Note: If refrigerated, allow the cartridge to come to room temperature in pouch
before use.
3.
Enter Required Information
Once the cartridge has been firmly inserted into the PTS Reader, the PTS
Reader prompts the user to enter the following information:
x
Enter OID
(Operator ID)
x
Enter Lot #
(Cartridge Lot #)
Note: If alternating between assay result formats, the user must delete all
stored lot numbers in the PTS Reader before initiating a test. This is done
by selecting Main Menu > #2 User Setup> #4 Erase Lot Numbers. Select
Enter to erase all stored lot numbers
x
Enter Calibration Code
(If the Calibration Code for the particular lot #
has already been entered, the PTS Reader does not prompt for the code
again. After deleting stored lot numbers in the PTS Reader, the user will
be prompted to enter in the calibration code)
x
Lot #
(Confirms cartridge lot number entered)
x
Enter Sample #1 Lot #
(This prompt is available for all four (4)
samples)
x
Enter Sample #1 ID
(Selecting and scrolling with the menu key under the
sample ID Header allows for fifty (50) samples to be entered and stored)
While the above information is being entered into the PTS Reader, the
cartridge is being pre-warmed.
4.
Dispense the Sample
Once all test information is entered, the PTS Reader displays:
x
ADD SAMPLE; PRESS ENTER
x
Pipette
25
μ
L of first sample into one (1) sample reservoir of the inserted
cartridge.
Change pipette tip to avoid cross-contamination between each
sample.
After all four (4) sample reservoirs are filled, press
Enter
on the
PTS Reader keypad.
x
Pumps draw sample aliquots into the test channels, thereby initiating the
test.
x
Results will be obtained in approximately 7 minutes for Gram-negative,
Gram-positive and Yeast/Mold identification and 3 minutes for Gram-
negative and Gram-positive identification.
