BRIGHT OTF6000, Руководство пользователя

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Bright Instruments 36
2.4.2 ANTI-ROLL ASSEMBLY
Refer to Figure 5.
The Anti-Roll plate is a device for ensuring
that tissue sections pass down the blade face
without curling, so that they can be collected
flat on to microscope slides. The setting
up of the Anti-Roll plate is second only in
importance to the quality of the blade edge in
obtaining ribbons of high quality sections.
Step 1: Preparation
a. Install a sharp Bright microtome knife,
setting the appropriate clearance angle for
that particular knife.
b. Clamp a frozen specimen (or embedding
compound alone) into the microtome (refer to
section 3.2)
c. Release the knife block clamp locking
lever and turn the knife carriage feed
control clockwise to bring the knife
towards the specimen.
d. Trim the specimen or embedding medium
until a suitable block face is made (refer to
section 3.3).
e. Lock the knife carriage clamp firmly
and proceed to cut sections at the chosen
thickness. Once it can be seen that regular,
good quality sections are cut (albeit curled
up) go on to step 2.
Step 2: Setting the Anti-Roll Plate
To ensure optimum sectioning performance
the anti-roll plate must be kept clean from
grease and dirt. To clean the anti-roll plate
carefully apply a small amount of methylated
spirits or ethanol to both the upper and lower
surfaces using a clean, dry, soft paper towel.
a. Place the Roll plate against the knife to
see where the Roll plate edge is positioned
in relation to the blade edge, the edge of the
Roll plate should be positioned slightly below
the knife edge, if this not the case, use the
adjustment knob to reposition.
NB:
if the edge of the Roll plate is above
the edge of the knife lift it from the
knife to adjust, dragging the Roll plate
downwards while on the knife could result
in damage to the Roll plate and knife).
b. Lightly tighten the Anti-Roll adjustment
lock so that the Anti-Roll adjustment knob
can still be turned.
c. Place the Roll plate back against the knife
and start to cut sections again. If the plate
is too low they will curl up on the tip of the
knife.
d. Slowly turn the anti-roll adjusting knob
clockwise while cutting sections. When the
plate reaches the correct height, the sections
will start to slide under the plate.
e. Tighten the Anti-roll adjustment lock then
flip the plate away from the knife. Now flip
the plate back into position. When cutting is
resumed, sections should go under the plate
as before, i.e. the plate has aligned itself. If
not, repeat the setting up process.
Your anti-roll plate may vary from the design
shown, but the set-up procedure remains
the same. Please refer to section 3.6, Frozen
Sectioning: A Problem Solving Guide, for
further assistance.
2. Operation Instructions
Cont.