Bright Instruments
45
4.4 MICROTOME KNIVES
Great care must be exercised when
handling knives:
• Knives must be stored in their boxes when
not in use
• Knives fitted to the microtome must be
properly guarded
• Particular care must be taken during
cleaning and knife sharpening
Conventional microtome knives are usually
made from carbon steel and will corrode in
moist conditions.
Whenever the cryostat chamber is allowed
to warm up above freezing point (e.g. during
a full defrost) the knife should be removed,
warmed up, cleaned and/or decontaminated,
oiled and then stored in its box in a dry place.
The Bright 50230 cryostat knife normally
supplied with the cryostat can be sharpened
on a conventional knife sharpening machine.
Alternatively, Bright Instrument Co Ltd offer a
knife sharpening service.
4.5 OPERATING IN EXTREME
CONDITIONS
The refrigeration system used in Bright
cryostats is highly efficient and will cope well
with high ambient temperatures. However,
where conditions of high humidity exist, it
may be necessary to adjust the automatic
defrost cycle in order to ensure that the
evaporator cooling fins remain frost free.
It is recommended that additional defrost
cycles are initiated (refer to section 2.1.11).
Do not exceed the defrost cycle time beyond
thirty minutes as this could cause the entire
chamber to warm up above 0ºC.
4.6 SERVICING AND REPAIRS
In the event of a breakdown a qualified person
should be called.
If a service visit is required, the cryostat
should be defrosted, decontaminated (refer
to section 4.8), cleaned thoroughly and left
switched off in preparation for that visit,
unless otherwise advised by the engineer.
Please remember that the cleaning procedure
should also include removing the microtome
and cleaning underneath where it sits in
the chamber - the microtome should be left
out of the chamber until the arrival of the
engineer. Failure to carry out this action will
result in the service visit being cancelled
and could incur further call-out charges. A
completed decontamination certificate must
be left with the cryostat and work will not
commence until the engineer has seen sight
of that certificate (refer to details regarding
decontamination certificates on the page
marked Safety Information towards the front
of this instruction manual).
4. Care & Maintenance
Cont.
Содержание OTF6000
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