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3.7 CUTTING TEMPERATURES

Standard textbooks on histological
technique give tables of recommended cutting
temperatures for different tissues. However, in
most cases, the following guidelines will
prove adequate.

3.7.1 FRESH UNFIXED TISSUE

Most soft tissues will cut at -18ºC to -20ºC.
Fatty tissue, such as breast lumps, will need
lower temperatures, -25ºC or colder. Brain and
spinal cord cut best at warmer temperatures,
e.g. minus 12ºC. Below is a table extracted
from IMVS Division of Pathology, The Queen
Elizabeth Hospital.

Sectioning temperature guidelines for fresh
unfixed tissue:
(Add 5°C to 10°C for fixed tissue.)

Tissue Type:

Temp. (ºC.)

Uterine curettings

-7

Brain

-10

Liver

-10

Spleen

-10

Testis

-10

Thyroid

-10

Lip

-13

Lymph Node

-15

Kidney

-15

Lung

-16

Muscle

-16

Connective Tissue

-16

Skin

-16

Heart

-18

Pancreas

-20

Uterus

-20

Tissue Type:

Temp. (ºC.)

Cervix

-20

Ovary

-20

Prostate

-20

Gut

-20

Bone Marrow

-20

Skin with fat

-25

Breast

-30

Omentum

-35

3.7.2 FIXED TISSUE

Differences between tissues are much reduced
after fixation. Cutting temperatures around
-10ºC to -15ºC are recommended, but some
experimenting may be required. Ensure
fixative is rinsed off tissue before freezing.

3. Cutting & Collecting

Sections Cont.

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Содержание OTF6000

Страница 1: ...Instruments Instruments OTF6000 Cryostat Manual ...

Страница 2: ... 14 2 1 Touchscreen 14 2 1 1 Lock Screen 14 2 1 2 Home Screen Low Spec 15 2 1 3 Home Screen High Spec 16 2 1 4 Temperature Settings Specimen 17 2 1 5 Temperature Settings Chamber 18 2 1 6 Motor Drive 19 2 1 7 Motor Drive Temps Screen 20 2 1 8 Settings User Account 21 2 1 9 Settings Admin Account 22 2 1 10 Settings UV Decontamination 23 2 1 11 Settings Defrost Control 24 2 1 12 Settings User Manage...

Страница 3: ... conditions 45 4 6 Servicing and Repairs 45 4 6 1 UK 46 4 6 2 Rest of world 46 4 7 Simple Servicing Repair 46 4 8 Decontamination 46 5 SPECIFICATIONS 48 5 1 Cryostat 48 5 2 5050 Microtome 49 ACCESSORIES 50 Product 51 Low Spec 51 High Spec 51 Equipment Options 52 Feed Screws for MAR Option 52 Orientation 52 Object Holders 52 Knives Blades 53 Anti Roll Plates 53 Ancillary Items 53 MATERIAL SAFETY DA...

Страница 4: ...Bright Instruments 4 SAFETY WARNING Low temperatures are present in this equipment Extreme care should be taken DO NOT let bare skin come into contact with metal surfaces ...

Страница 5: ...ARNING Extremely sharp knives blades Use knife blade guards at all times Use correct tools for removal and insertion of knives blades DO NOT leave knives blades laying around Place knives blades not in use into box wallet provided ...

Страница 6: ...d This cryostat is fitted with an in line mains filter which may affect portable appliance test results b Fuses Fuse ratings are clearly indicated on all fuse panels adjacent to the fuse holder If and when replacement is necessary the correct fuse rating must be adhered to c Earthing Grounding A protective earth terminal is fitted and must be used in all two wire installations NB It is recommended...

Страница 7: ...it is cold as well as sharp Always use knife guards When placing object holders in the microtome when orientating manipulating or in any way placing fingers in a position above the knife edge ensure that the handwheel is locked and knife guards are in position All Bright Instrument Company Limited personnel are encouraged to make suggestions regarding product safety We also welcome such suggestion...

Страница 8: ...nts or parts returned to us should be accompanied by a completed decontamination certificate A copy of this can be found at the back of this instruction manual and we suggest you use a photocopy of this when returning parts b Alternatively you can download a decontamination certificate from our website c Should the instrument or any part of it be received in a condition that Bright Instrument Comp...

Страница 9: ...o a period of six months from the date when the goods shall have been re delivered to the Buyer c This warranty does not apply to any defects caused by wear and tear incorrect installation abnormal conditions of working accident misuse or neglect d That save as in this clause herein before expressed the Seller shall not be under any liability for negligence or otherwise in respect of defects in go...

Страница 10: ...factory The following instructions are given for the re assembly of the instrument adjustments and its correct use If the instrument is received before preparations for installation are completed It should be stored in a clean dry place and not exposed to dirty or damp conditions 1 1 2 RECEIPT Immediately upon receipt of the instrument make a careful examination for evidence of damage encountered ...

Страница 11: ...aboratory air The drain plug should be left in position except when cleaning is carried out 1 2 3 FROST ELIMINATION COVER Place the provided Frost Elimination cover onto the Freezer Plate The cover stops the build up of frost on the Freezer Plate Freezer Plate Frost Elimination Cover 1 2 4 POSITIONING The instrument should be positioned on a level floor To ensure adequate ventilation leave a gap o...

Страница 12: ...ation as pictured so the microtome can slide into position Carefully push the microtome back aim the back feet between the guides that will guide the foot to the pin push until it won t go back any further it should fit snugly into position Microtome coupling Drive Shaft coupling S l i d e o n t o c Plug the microtome into the socket located on the right hand side of the chamber Line up white dash...

Страница 13: ...eck the voltage stamped on the nameplate located on the back of the cabinet with your supply The connections are Brown Positive live Blue Negative neutral Yellow Green Earth ground NB It is recommended the OTF6000 is plugged in via an electrical surge protection unit 1 3 3 ELECTRICAL SAFETY Where earth cables may have to be removed from panels for servicing or repair purposes care should be taken ...

Страница 14: ... Temperature DECONTAMINATION DUE Present Tag To Start Cryostat OTF6000 Instruments 12 6 C Chamber Temperature DEFROSTING Present Tag To Start Cryostat OTF6000 Instruments 10 5 C Chamber Temperature HIGH TEMP ALARM Live chamber temperature readings Relevant task warning messages NOTE Window lock is active when this screen is displayed To unlock either present RFID key fob or tap anywhere on touch s...

Страница 15: ...Instructions Cont Rewind position indicator Turns red when furthest back Advance position indicator Turns red when furthest forward Sign out Live reading of Specimen Temperature Touch to set target Live reading of Quick Freezer bar temperature Live reading of Chamber Temperature Touch to set target Cut Counter Touch to reset Settings Menu Goes green when the microtome head is at start of retractio...

Страница 16: ...CHSCREEN CONT 2 1 3 HOME SCREEN HIGH SPEC ONLY 2 Operation Instructions Cont Defrost Schedule Confirmed Sign out Live reading of Specimen Temperature Touch to set target Live reading of Quick Freezer bar temperature Live reading of Chamber Temperature Touch to set target Settings Menu Press to enter Cutting Zone controls Turn light on off ...

Страница 17: ... Fatty Harder Soft Brain 19 5 C Specimen Temperature Target Specimen Temperature Return to Home Screen Increase Target Specimen Temperature Decrease Target Specimen Temperature Live reading Current Specimen Temperature Recommended Preset Cutting Temperatures Selecting will change both specimen and chamber temperatures to the preset parameters ...

Страница 18: ...ts Fatty Harder Soft Brain 23 0 C Chamber Temperature Recommended Preset Cutting Temperatures Selecting will change both chamber and specimen temperatures to the preset parameters Target Chamber Temperature Increase Target Chamber Temperature Live reading Current Chamber Temperature Return to Home Screen Decrease Target Chamber Temperature ...

Страница 19: ...Screen Slow Speed Rewind Fast Speed Advance Fast Speed Rewind Slow Speed Advance Shows Temps Screen readings See next page Switch between Continuous and Single cutting Stop and Start the microtome cutting Rewind position indicator Turns red when furthest back Advance position indicator Turns red when furthest forward CAUTION To avoid mechanical damage before using the advance rewind feature set ei...

Страница 20: ...ck Advance position indicator Turns red when furthest forward Sign out Live reading of Specimen Temperature Live reading of Quick Freezer bar temperature Live reading of Chamber Temperature Cut Counter Touch to reset Settings Menu Turn light on off 2 1 TOUCHSCREEN CONT 2 1 7 MOTOR DRIVE TEMPS SCREEN HIGH SPEC ONLY 2 Operation Instructions Cont Goes green when the microtome head is at start of retr...

Страница 21: ...Defrost duration and time settings Return to Home Screen HIGH SPEC ONLY Select machine to run in either single or dual compressor mode When running in Single mode the cryostat will automatically switch between one compressor and the other in order to reduce wear on the compressors Please note that there will be a pause of 40 to 70 seconds between the compressors starting in order to reduce current...

Страница 22: ...d Log Exit UV Decontamination Defrost Control User Management Single Dual Compressor Settings Contains directory of log in data Defrost duration and time settings Set time and date Return to Home Screen UV Decontamination duration settings HIGH SPEC ONLY Select machine to run in either single or dual compressor mode ...

Страница 23: ...lected a Decontamination Scheduled message will appear on Home Screen It won t begin until the Users session has ended and the window is closed This button only appears once a duration has been selected Press to cancel selected duration Select a decontamination duration CAUTION UV C radiation is dangerous and excess exposure can cause skin and eye damage For safety purposes the UV Decontamination ...

Страница 24: ... 1 11 SETTINGS DEFROST CONTROL 2 Operation Instructions Cont Return to Home Screen Once duration is set Select single double or triple defrost by touching one of the fixed time buttons Once selected a Cancel button will appear in the top left corner of the screen Slide to adjust duration Goes on straight away 4 hour process for total defrost of chamber from frozen ...

Страница 25: ...Space 123 Present RFID Tag to assign to Tony Atkins Instruments 355355 Your Unique PassCode Skip Select Create New User and type in a new user name To confirm name press the return button When user name is entered a unique user code is generated At this stage a blank RFID key fob can be placed on the RFID key fob scanner to the right of the screen to program the fob for this user If you are not us...

Страница 26: ...Tony Atk t y u w e q r i o p v b n z x c m f g h a s d j k l Space 123 Present RFID Tag to assign to Tony Atkins Instruments 355355 Your Unique PassCode Skip Select Edit User and then select the user you would like to edit Type in a new user name To confirm name press the return button Return to Home Screen A new unique passcode is then generated At this stage a blank RFID key fob can be placed on...

Страница 27: ... Exit User Management Select Delete User and then select the user you want to delete Once you have selected a user a delete button will appear Press to delete Return to Home Screen Exit Delete Administrator Diana R Ian W Luke G Michael K Tony A 355355 3403401 599599 615615 3143149 363363 A6B1D7D818000000 Linda H 355389 18B1D7C618000000 169AD9D88D000000 User Delete ...

Страница 28: ...ht Instruments 28 2 1 TOUCHSCREEN CONT 2 1 13 SETTINGS SET CLOCK ADMIN ONLY 2 Operation Instructions Cont Discard Save Set Clock 14 38 23 09 20 Save changes to date and time Return to Home Screen Time Date ...

Страница 29: ...8 14 30 35 14 35 15 NoUS U02 U99 NoUS NoUS NoUS U00 NoUS QF 26 4 QF 28 8 QF 30 8 QF 0 0 QF 0 0 QF 8 8 QF 30 8 QF 29 3 SP 16 4 SP 18 8 SP 20 8 SP 0 0 SP 0 0 SP 1 8 SP 20 8 SP 19 3 CH 19 4 CH 21 8 CH 23 8 CH 0 0 CH 0 0 CH 5 8 CH 23 8 CH 22 3 User LOGIN Tag U02 User END Session CTRL User IDLE Timeout System Power ON System Power ON User LOGIN Passcode U00 User END Session CTRL User IDLE Timeout Event...

Страница 30: ...everts to full screen showing positive and negative temperatures 2 1 TOUCHSCREEN CONT 2 1 14 SETTINGS EVENT LOG GRAPH ADMIN ONLY 2 Operation Instructions Cont Graph Specimen Chamber Quick Freeze Full Scale Exit Negative Positive 1125 min period 19 08 20 12 45 48 20 08 20 07 32 12 40 30 20 10 10 20 30 40 Y Axis Temperature X Axis Time ...

Страница 31: ...Bright Instruments 31 RFID Key Fob Scanning Hover key fob over area Emergency Stop High Spec Models Only USB 2 2 CABINET CONTROLS 2 2 1 AROUND TOUCHSCREEN Refer to Figure 1 2 Operation Instructions Cont ...

Страница 32: ...e set chamber temperature typically 10ºC below chamber temperature and is useful for storage or pre cooling of knives etc Although it is possible to freeze fresh tissue on the quick freezer plate it is generally preferable to use a much colder medium The temperature of the quick freezer plate is displayed constantly on the main control panel Do not forget to replace the frost elimination cover aft...

Страница 33: ... press the switch in the intended direction 2 2 6 FOOT SWITCH The Foot switch is only available on High Spec models and is used to control the Motor drive The Motor drive has two different modes of operation they are single cut and continuous cutting mode the mode being selected through on the touchscreen On single cut mode pressing the foot switch commences a single cut While on continuous cuttin...

Страница 34: ...ee the table below for a list of recommended knife angles Part No Description Angle 2 5 50230 Standard knife 15º 50232 1 Hardened steel knife 12º 50207 Steel knife 15º 50234 Tungsten carbide tipped knife 25º 2 3 3 ANTI ROLL ASSEMBLY Refer to Figure 3 The Anti Roll plate is a device for ensuring that tissue sections pass down the blade face without curling so that they can be collected flat on to m...

Страница 35: ...ngle between the bottom face of the plate and the blade set the angle somewhere between 10 15 e Slowly turn the anti roll adjusting knob clockwise while cutting sections When the plate reaches the correct height the sections will start to slide under the plate f Tighten the Anti roll adjustment lock then flip the plate away from the knife Now flip the plate back into position When cutting is resum...

Страница 36: ...its or ethanol to both the upper and lower surfaces using a clean dry soft paper towel a Place the Roll plate against the knife to see where the Roll plate edge is positioned in relation to the blade edge the edge of the Roll plate should be positioned slightly below the knife edge if this not the case use the adjustment knob to reposition NB if the edge of the Roll plate is above the edge of the ...

Страница 37: ...to turn anticlockwise will move the knife away from the specimen When set in the desired position lower the knife block clamp lever to lock in position 2 5 2 SECTION THICKNESS CONTROL To select section thickness set the thickness control at zero and rotate anticlockwise until desired thickness is level with the indicator To reset the section thickness at any time ensure that the thickness control ...

Страница 38: ...ON DIOXIDE SOLID This can be used in a bath of solvent such as hexane to achieve temperatures around 60ºC Safer than CO2 gas solid CO2 cardice remains one of the most popular methods of freezing 3 1 4 FREEZER PLATES Some cryostats incorporate special cold plates for rapid freezing covering the temperature range 45ºC to 60ºC The OTF cryostat has a standard quick freezer plate that operates at appro...

Страница 39: ...lour Watch the freezing isotherm until just before it reaches the top surface of the embedding compound and while it is still liquid carefully apply some more embedding compound until it forms a mound on the top Then let it continue to freeze until it is completely solid c Remove the object holder from the quick freezer and place it in the microtome NOTE 1 If you are unlucky enough to have your ob...

Страница 40: ...anti roll plate against the knife and proceed to cut sections if adjustments to the anti roll plate are required see section 2 3 3 d To collect sections have a clean microscope slide ready at ambient temperature Bring the slide towards the knife swing the anti roll plate out of the way and move the slide very close to the section s The section s should jump across the gap and stick to the slide NB...

Страница 41: ...advice in section 3 6 below 3 6 FROZEN SECTIONING A PROBLEM SOLVING GUIDE Cutting good frozen sections requires skill and practice and some tissues are particularly difficult In all cases the quality of the result depends on a number of factors The notes which follow deal with the more commonly encountered difficulties and suggest a systematic approach to problem solving 3 6 1 SOLUTION CHECKLIST F...

Страница 42: ...trimmed correctly knife not sharp enough knife edge too thin cutting motion uneven Vertical score marks on sections Knife edge nicked or chipped Knife faces have built up debris or frost Anti roll plate edge damaged Anti roll plate surface has debris or frost built up Tissue contains hard fine particles e g bone fragments NB When removing frost and debris always brush up the knife never down or al...

Страница 43: ...hology The Queen Elizabeth Hospital Sectioning temperature guidelines for fresh unfixed tissue Add 5 C to 10 C for fixed tissue Tissue Type Temp ºC Uterine curettings 7 Brain 10 Liver 10 Spleen 10 Testis 10 Thyroid 10 Lip 13 Lymph Node 15 Kidney 15 Lung 16 Muscle 16 Connective Tissue 16 Skin 16 Heart 18 Pancreas 20 Uterus 20 Tissue Type Temp ºC Cervix 20 Ovary 20 Prostate 20 Gut 20 Bone Marrow 20 ...

Страница 44: ...ll plate c Remove the microtome knife refer to section 2 3 2 and clean it see section 4 4 d Remove the microtome from the chamber the reverse of the installation procedure see section 1 2 5 Remove drain plug from base of chamber e Place the microtome on the laboratory bench and clean it Refit the rewind knob and rewind the feed mechanism until the pointer is at the bottom on the indicator Undo the...

Страница 45: ...cycle in order to ensure that the evaporator cooling fins remain frost free It is recommended that additional defrost cycles are initiated refer to section 2 1 11 Do not exceed the defrost cycle time beyond thirty minutes as this could cause the entire chamber to warm up above 0ºC 4 6 SERVICING AND REPAIRS In the event of a breakdown a qualified person should be called If a service visit is requir...

Страница 46: ...e extended warranties and a full range of service contracts For further information or for any refrigeration electrical or mechanical problems contact Bright Instrument Co Ltd direct providing the following information Cabinet Number see ID plate on rear panel Microtome Number see ID plate on rear panel Date of installation Nature of fault 4 6 2 REST OF THE WORLD Refrigeration problems are likely ...

Страница 47: ...0ml of formalin BP in a flat dish inside the chamber Close the window c Leave for at least 24 hours preferably 48 hours d Open the window and place a beaker containing 10ml of ammonia SG 880 in the chamber Close the window e Leave for one hour The cryostat is now decontaminated For further information regarding alternative decontamination procedures please refer to Safe Working and the Prevention ...

Страница 48: ...h Yes Yes Manual Handwheel Drive Yes Yes Motorised Handwheel Drive No Yes Emergency Stop No Yes Built In Footswitch No Yes Refrigeration 35 Single Compressor 40 Dual Compressor Quick Freeze Block Yes Yes Anti Fog Window Yes Yes Window Lock RFID Access Yes Yes Built In Waste Collection Bottle Yes Yes Heated Specimen Block Yes Yes UV Decontamination Yes Yes Refrigeration Alarm Yes Yes Recent User Lo...

Страница 49: ...utting zone single or continuous modes hands off foot switch and emergency stop Safety Features Counter balance handwheel with safety lock Built in knife guards Locking window Requires RFID tag or password to access the touch screen controls Dimensions H300 x W300 x D500mm Weight Net weight Manual 20kg Gross weight Manual 25kg Construction Rotary rocking design of cast alloys with detergent and so...

Страница 50: ...Bright Instruments 50 Accessories ...

Страница 51: ...ratch and solvent resistant Standard accessories include 50230 Solid Knife or Feather Blad Easi set anti roll plates 50mm Circular object holders 2 x 22mm 1 x 37mm Set of Allen keys Bottle of Cryo M Bed 113ml Brush and debris tray Debris brush Instruction manual LOW SPECIFICATION OTF6000 LS 001 220V 240V AC 50 60Hz Solid blade OTF6000 LS 002 110 115V AC 50 60Hz Solid blade OTF6000 LS 003 for 220V ...

Страница 52: ...r for use with item 51695 50221 Quick release object holder clamp complete with 50 metal object discs 50727 Hollow object holde 22mm 50690 Metal object holder discs 22mm pack of 50 50204 Cork object holder discs 22mm pack of 50 50204 1 Cork object holder discs 50 x 50mm pack of 50 54168 Prostate Cryomould Former L22 x W6 x D4mm 54170 Quick release object holder strip clamp complete with 10 of part...

Страница 53: ...uitable for cutting bone and hard plastics NB Needs to be resharpened at our factory ANTI ROLL PLATES Code Description 52485 Easi Set anti roll plate 50mm ANCILLARY ITEMS Code Description 57808 Anti static brush 12mm 57344 Knife cleaning brush 53581 Bright Cryo M Bed 120ml carton of 6 bottles 53581 1 Bright Cryo M Bed 120ml bottle 57713 1 Bright Cryospray 134 300ml areosol can 57713 Bright Cryospr...

Страница 54: ...Bright Instruments 54 Material Safety Data Sheets ...

Страница 55: ...dable miscible in all proportions Transport Information No restrictions Hazards Identification Skin Can cause skin irritation Respiratory May cause difficulty in breathing if exposed to very high concentration Ingest May be harmful by ingestion Eyes Can cause Eye irritation First Aid Procedures Skin Wash thoroughly with soap and water Respiratory Move to fresh air Ingest Rinse mouth out with water...

Страница 56: ...gas mist vapours spray P308 P313 If exposed or concerned Get medical advice attention P410 P403 Protect from sunlight Store in a well ventilated place Potential Health Effects Skin Rapid evaporation of the liquid may cause frostbite Eyes May irritate eyes Ingestion Unlikely route of exposure Inhalation Inhalation may cause central nervous system effects Vapours may cause dizziness and drowsiness C...

Страница 57: ...eavier than air and can cause suffocation by reducing oxygen available for breathing Avoid skin contact with leaking liquid danger of frostbite Use personal protective equipment Keep people away from and upwind of spill leak Environmental precautions Prevent further leakage or spillage if safe to do so The product evaporates readily Prevent spreading over a wide area e g by containment or oil barr...

Страница 58: ...cordance with EN standards respirator EN 136 140 149 safety glasses EN 166 protective suit EN 340 463 468 943 1 943 2 gloves EN 374 safety shoes EN ISO 20345 The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace Avoid inhalation of vapour or mist Environmental exposure controls Avoid breathing vapours mist o...

Страница 59: ...ng materials Further information Provisions relating to waste EC Directive 2006 12 EC 91 689 EEC Regulation No 1013 2006 12 Ecological Information Persistence and degradability Biodegradability Aerobic Result Not readily biodegradable Ecotoxicity effects Effects Species Value Exposure time Comments Toxicity to fish NOEC Cyprinus Carpio Carp 117 mg l 96 h Toxicity to aquatic plants NOEC Algae 170 m...

Страница 60: ...ene Ems number F C S V Marine pollutant No 15 Regulatory information Safety health and environmental regulations legislation specific for the substance or mixture Other inventory information Country Legislation Information US Toxic Substances Control Act On TSCA inventory Australia Industrial Chemical Notification Assessment Act Not in compliance with inventory Canada Canadian Environmental Protec...

Страница 61: ...en as the decimal sign and its position complies with rules for the structure and drafting of international standards and is a comma on the line As an example 2 000 is two to three decimal places and not two thousand whilst 1 000 is one thousand and not one to three decimal places This data sheet contains changes from the previous version CLP03 dated January 2018 Sections 1 and 9 have been updated...

Страница 62: ...al and chemical properties Form Liquid Density 15ºC Kg 1 0 878 Odour Mineral oil odour Colour Pale amber Flashpoint 153ºC IP 34PM closed cup Stability and reactivity Stable Yes Conditions to avoid Extreme temperatures store between 0 50ºC Materials to avoid Strong oxidising agents Hazardous Decomposition Combustion Products Dependant of conditions bringing about decomposition the following substan...

Страница 63: ...ngest DO NOT induce vomiting Wash out mouth with water SEEK MEDICAL ATTENTION URGENTLY Eyes Flush copiously for at least 15 minutes If irritation persists SEEK MEDICAL ADVICE Fire fighting measures Extinguish fires with foam dry powder CO2 or water fog do not use water jets Toxicological information This product is NOT classified as dangerous for supply or conveyance Accidental release measures Le...

Страница 64: ...Bright Instruments 64 References Drawings ...

Страница 65: ...inet 1 Advance Rewind Switch 2 Automatic Demist Window 3 USB 4 Storage 5 Touchscreen 6 RFID Key Fob Scanning 7 Emergency Stop High Spec Models Only 8 Handwheel 9 Waste Liquid Collection 10 Foot Switch High Spec Models Only 1 2 3 4 5 7 6 8 9 10 ...

Страница 66: ...l Adjustment Lock 4 Anti Roll Angle Adjustment Bracket 5 Anti Roll Angle Adjustment Knob 6 Feed Indicator 7 Knife Guard 8 Manual Advance Rewind Plug 9 Anti Roll plate 10 Solid Knife 11 Knife Locking Knob 12 Knife Block Clamp Locking Lever 13 Section Thickness Control 14 Coarse Advance Control 1 2 3 4 5 6 7 14 13 12 11 10 8 9 ...

Страница 67: ...E ALL BURRS SHARP EDGES AND CORNERS SURFACES TO BE CLEAN AND SCRATCH FREE Figure 3 5050 Microtome Solid Knife Anti Roll Assembly Tighten to lock Anti Roll plate in position Loosen to set the angle of the Anti Roll plate then tighten to hold in position Turn Dial Turn Dial Move Anti Roll plate forwards Move Anti Roll plate backwards ...

Страница 68: ... Roll Adjustment Knob 3 Anti Roll Adjustment Lock 4 Blade Clamping Plate 5 Feed Indicator 6 Manual Advance Rewind Plug 7 Anti Roll Plate 8 Blade Loading Plate 9 Blade Guard 10 Blade Clamping Lever 11 Knife Block Clamp Locking Lever 12 Section Thickness Control 13 Coarse Advance Control QRFBH 6 1 2 3 4 5 7 13 11 8 9 12 10 ...

Страница 69: ...ARP EDGES AND CORNERS SURFACES TO BE CLEAN AND SCRATCH FREE Figure 5 5050 Microtome Quick Release Feather Blade Holder QRFBH Anti Roll Assembly Tighten to lock Anti Roll plate in position Move Anti Roll plate forwards Move Anti Roll plate backwards Turn Dial Turn Dial ...

Страница 70: ... Company Serial No Product No Telephone Email Order No Blood body fluids pathological samples Other biohazards Other hazards Chemicals substances hazardous to health Mark Box A if applicable Otherwise complete all parts of B providing further information as requested or appropriate Further Details Further Details Yes No Yes No Yes No Yes No A This equipment has not been in contact with unfixed bio...

Страница 71: ... of the QA Manager Name Address Company Department Postcode Serial No Telephone PURCHASING Did the purchasing process run smoothly with respect to our involvement e g correct advice lead times payment arrangements etc MISCELLANEOUS Any other aspect you would like to comment on e g appearance first impressions etc SAFETY Any comments PURCHASING Did you receive an operating manual Do you believe it ...

Страница 72: ...mber of Instruments Instruments Instruments Instruments Instruments Burnett House Lakeview Court Ermnie Business Park Huntingdon Cambridgeshire PE29 6UA 44 0 808 168 9697 brightinstruments com Instruments ...

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