Biotek Synergy HTX, Руководство оператора

Страница: 59 / 184

Key Components |
37
2.
Properly orient the filter or plug (see page 34), and then drop it into the
desired filter wheel location.
3.
Using your fingers, squeeze the sides of the C-clip filter retainer, and then
insert it into the top of the hole containing the new filter. Cover your finger
with several layers of lens paper, and then push down on all sides of the C-
clip until it sits flush against the filter.
4.
Clean both sides of the filter with lens paper.
To reinstall a filter wheel:
1.
Ensure that all filters and/or plugs are inserted properly (see above).
2.
Slide the filter wheel back into its chamber.
3.
Replace the thumbscrew.
4.
Close the front door.
5.
Turn on the instrument.
Installing the Time-Resolved Fluorescence Cartridge
For Synergy HTX models that support time-resolved fluorescence, the “TR” cartridge
must be installed in place of the excitation filter wheel before a TRF assay can be run.
The TR cartridge allows light from the xenon flash bulb to be input to the fluorescence
optical system within the Synergy HTX. Excitation wavelengths are selected by
adjusting the monochromator from 200 to 999 nm in 1-nm increments, with a fixed
bandwidth of 10 nm.

The Synergy HTX automatically detects the presence of the TR
cartridge. At the start of a time-resolved fluorescence assay, the
operator will be prompted to install the TR cartridge if it is missing.
1. Important!
Turn off the instrument.
2.
Open the filter wheel access door using the depression on the right side of
the door. Observe the two thumbscrews within the compartment. The left
thumbscrew holds the Excitation filter wheel in place. See the figure on
page 33.
3.
Remove the left thumbscrew and slide the filter wheel’s supporting metal
bracket straight out of the compartment.
4.
Slide the TR cartridge into the compartment and replace the thumbscrew.
Close the front door and turn on the instrument.

See page 43 for more information on creating Gen5 protocols.
Synergy HTX Operator's Manual