Biotek Synergy HTX, Руководство оператора

Страница: 159 / 184

Dispense Module Tests |
137
Results Analysis

For your convenience, we’ve included a
worksheet
at the end of this
chapter for recording the dispense weights, Delta OD values, calcula-
tions, and pass/fail. Make two copies of this worksheet—one for each
dispenser tested.
The pass/fail criteria for each set of 32 wells with the same dispense volume is
based on the calculated coefficient of variation (%CV) and Accuracy % Error.
For each volume dispensed (80, 20, 5 µL), for each dispenser (1, 2):
•
Calculate the Standard Deviation of the 32 wells
• Calculate the Mean of the 32 wells
• Calculate the %CV:
(Standard Deviation / Mean) * 100
•
Calculate the Accuracy % Error:
((Actual Weight – Expected Weight) / Expected Weight) * 100

Expected Weights
for 32 wells: 80 μ L (2.560 g), 20 μ L (0.640 g), 5 μ L
(0.160 g). It is assumed that one gram is equal to one milliliter.
Dispense
Volume
To pass,
% CV must be:
To pass, Accuracy
% Error must be:
80 μL ≤ 2.0% ≤ 2.0%
20 μL ≤ 7.0% ≤ 5.0%
5 μL ≤ 10.0% ≤ 20.0%
Failures
If any tests fail, prime the fluid lines and rerun the test(s). If the test(s) fail
again, the injector heads may require cleaning (see
Preventive
Maintenance
).
If tests continue to fail, contact BioTek’s Technical Assistance Center.
Gen5 Test Protocols for Models with Absorbance Capabilities

Perform these steps to create a protocol to test Dispenser 1. Then,
open a copy of the protocol and change the relevant parameters for
Dispenser 2.
1.
Select
System > Instrument Configuration
, and add/configure the
Synergy HTX
(if it is not already there).
Synergy HTX Operator's Manual