Biotek Synergy HTX, Руководство оператора, Страница: 128 / 184

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Chapter 6: Instrument Qualification
Luminescence Tests
For Synergy HTX models with luminescence capability, you can use a Harta Luminometer
Reference Microplate, which is an LED-based test plate. Contact BioTek to purchase a
plate, or go to www.hartainstruments.com for more information.
Harta Plate Test
Materials
• Harta Luminometer Reference Microplate, PN 8030015
• Harta plate adapter, PN 8032028
•
Gen5 protocol (see page 107)
Procedure
1.
Turn on the Harta reference plate using the I/O switch on the back of the plate.
2.
Check the plate’s battery by pressing the test button on the back of the plate
and ensuring that the test light turns on.

The test light may be difficult to see in bright light. Change your angle
of view or move to a darker environment if you cannot see it.
3.
Place the Harta plate adapter on the reader’s carrier and then place the test
plate on top of the adapter.
4.
Create an experiment based on
Synergy HTX LumTest_Harta.prt
protocol,
and read the plate.
5.
Calculate and evaluate results as described under
Results Analysis
below.
Plate Layout
1 2 3 4 5 6 7 8 9 10 11 12
A
A2
meas
battery
check
battery
check
B
C
D
E
F buffer buffer buffer buffer buffer buffer buffer buffer buffer buffer buffer buffer
G buffer buffer buffer buffer buffer buffer buffer buffer buffer buffer buffer buffer
H
BioTek Instruments, Inc.