Biotek Synergy HTX, Руководство оператора

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Fluorescence Tests |
121
•
Are you using new/clean plates? We suggest you rerun the test with a
new/clean microplate. For the bottom optics test, if the base of a plate is
touched, clean the entire base with alcohol (95% ethanol) and then wipe with a
lint-free cloth. Before placing a plate in the instrument, blow the bottom of the
plate with an aerosol. If the test fails again, the optical probe(s) may need to be
cleaned. Contact BioTek TAC for instructions.
•
If the Corners Test continues to fail, the hardware may be misaligned. Contact
BioTek TAC.
•
Review the instructions under “Pipette Map” (page 121 for SF or 128 for MUB)
to verify that you correctly prepared the plates.
• Does the Plate Type setting in the protocol match the plate you used?
Pipette Map

Seal the plates with foil or store them in black polyethylene bags until use.
If the base of a plate is touched, clean the entire base with alcohol (95%
ethanol) and then wipe with a lint-free cloth. Before placing a plate in the
instrument, blow the bottom of the plate with an aerosol duster.
Perform these steps carefully and refer to the grid on the next page.
For the
Corners
test (light gray wells):
• Pipette 200 µL of the
3.3 nM SF
solution into wells A1-A3, A10-A12,
H1-H3, and H10-H12.
•
If using a Hellma plate:
Pipette 200 µL of buffer into the wells surrounding
the 3.3 nM wells (“ CBUF ” in the grid).
For the
Sensitivity
test (dark gray wells):
• Pipette 200 µL of the 1 nM SF solution into well D7.
•
Pipette 200 µL of the buffer solution into wells C9, D9, and E9.
For the
Linearity
test (wells C1–F5):
• Use a multichannel pipette with just four tips installed.
•
Pipette 150 µL of buffer solution into wells C2-F5. Discard the tips.
•
Pipette 150 µL of the
1 nM SF
solution into wells C1-F1.
• Pipette 150 µL of the 1 nM SF solution into wells C2-F2.
Mix the wells using the pipette. Do not discard the tips.
•
Aspirate 150 µL from wells C2-F2 and dispense into wells C3-F3.
Mix the wells using the pipette. Do not discard the tips.
•
Aspirate 150 µL from wells C3-F3 and dispense into wells C4-F4.
Mix the wells using the pipette. Do not discard the tips.
Synergy HTX Operator's Manual