Biotek Synergy HTX, Руководство оператора, Страница: 127 / 184

Absorbance Plate Test |
105
•
Alignment:
This portion of the test measures the alignment of the microplate
carrier with the optical path. A reading greater than 0.015 OD in the four corner
holes represents an out-of-alignment condition.
If the test fails:

Ensure that the Test Plate is correctly seated in the microplate carrier.

Check the four alignment holes (A1, A12, H1, H12) to ensure they are
clear of debris.

Check the microplate carrier to ensure it is clear of debris.

If the problem continues to reoccur, contact BioTek TAC.
•
Accuracy:
Accuracy is a measure of the optical density of the neutral-density
filters in wells C1, D4, E2, F5, G3, and H6 as compared with known standard
values contained in the test plate’s data sheet.
Since there are several filters with differing OD values, the accuracy across a
range of ODs can be established. Once it is proven that the reader is accurate at
these OD values, the reader is also considered linear. To further verify this, you
can perform a regression analysis on the Test Plate OD values in a program
such as Microsoft Excel. An R Square value of at least 0.990 is expected.
If the test fails:

Check the filters in the Test Plate to ensure they are clean. If necessary,
clean them with lens paper. Do not remove the filters from the test plate,
and do not use alcohol or other cleaning agents.

Verify that the filter calibration values entered in Gen5 are the same as
those on the Test Plate’s data sheet.

Verify that the Test Plate is within its calibration certification period. The
calibration sticker is affixed directly to the plate. If it is out of date, contact
BioTek to schedule a recertification.
• Repeatability:
Repeatability is a measure of the instrument’s ability to read
the same well with minimum variation between two reads with the well in the
same location.
If the test fails:

Check the neutral-density filters on the Test Plate to ensure there is no
debris that may have shifted between readings and caused changes.

Check the microplate carrier to ensure it is clear of debris.
Synergy HTX Operator's Manual