Biotage Selekt Enkel, Руководство пользователя

Страница: 14 / 44

12 Biotage
®
Selekt Enkel User Manual | © Biotage 2022
Start, Monitor, and Control a Purification
Chromatogram
The signals and the thresholds are displayed in the
chromatogram using the following colors:
= Light absorption measured by the UV detector for
the whole
λ
-All range and the threshold in mAU.
(Requires a Spektra software license.)
= Light absorption measured by the detector
at wavelength UV1 and the threshold in mAU.
= Light absorption measured by the UV detector
at wavelength UV2 and the threshold in mAU.
The defined wavelengths and real-time measurements of
the absorption are displayed in the
Collection panel.
Zoom in on the Chromatogram
It is always possible to zoom in and out of the gradient and
chromatogram using the pinch-to-zoom feature (see Figure 27).
It is possible to zoom in one direction (X or Y) or both directions
(X and Y). To reset the zoom, press .
Figure 27. Zoomed-in chromatogram.
Current Solvent Mix
The percentages of pumped solvents are displayed underneath
the chromatogram during the entire run (see Figure 25 and
Figure 26).
Status in the Top Pane
»
Equilibration Flush:
The system empties the
solvent inlets of solvents used in the previous
purification and fills them with new solvents.
»
Equilibration:
The system is running a column equilibration.
»
Purge: The system releases the column pressure.
»
Sample Load:
Sample can be loaded onto the column.
»
UV Warm-Up: The UV lamp is being warmed up.
»
UV Zero:
The system is setting the UV zero level using
the solvent mix used at the start of the gradient.
»
Baseline Detection: The system is measuring the
light absorbance of the used solvents (A and B)
for the whole detector range. During the gradient
run, the baseline is subtracted from the signal.
(Requires a Spektra software license.)
»
Baseline Flush: After baseline detection,
the system is flushed with the solvent mix
used at the start of the gradient.
»
Gradient: The system is running a purification.
»
Line Flush, Purge, and Detector Flush: At the end
of a run, i.e. after the gradient purification stage is
completed or ended by the user, the system performs
the flushes that are enabled in the system settings
(see page 24) and a system decompression (purge).
»
Finished: The purification run was completed
or ended/aborted by the user.
»
Failed: The purification run failed.
Manual UV Zero
During the gradient run, it is possible to manually set the
current UV absorbance level to zero AU by pressing in the
chromatogram. This feature can be enabled/disabled in the
system settings; see page 24.
Note: For systems with Spektra software license, the button
is only enabled when UV Baseline Correction is turned off in
the run setup.
Start and End an Isocratic Segment
At any time during the gradient run, you can start an isocratic
segment by enabling the
Hold option in the chromatogram
(see Figure 27). End the segment by disabling the option.
Note: When using the default system settings, the isocratic
hold is limited to run for a maximum of 10 CV at the time
(see page 24). After 10 CV, the run is paused and you can
either resume the isocratic hold for up to another 10 CV or end it.
Add, Empty, and Replace
Racks During the Run
Note:
To add/remove racks during a run, the run must
be paused.
If more fractions are to be collected than can fit in the available
rack(s), the system automatically pauses, the collection arm
returns to the home position (the inner right corner), and you
are prompted to load and/or assign more racks to the run.
To assign a rack to the run, expand the Rack panel and set the
check box in front of the rack.
Note: Rack sharing with another run is only possible at the
start of a run; see “Rack Sharing” on page 10.