Biotage Selekt Enkel, Руководство пользователя

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11
Start, Monitor, and Control a Purification
Start, Monitor, and Control a Purification
Warning
»
Read and follow the safety precautions against static electricity
in the Biotage
®
Selekt Family Installation and Safety document
(P/N 417511) that is supplied with the system.
»
Always ensure that the settings for the column selected in the
software are correct for the column to be used before starting
a run. Never exceed the maximum pressure or flow rate for the
used column.
»
Never use the system without a column mounted or the column
inlet and outlet tubing coupled together.
»
Keep hands away from the collection arm area until the arm has
returned to its home position (in the inner right corner) and the
system is paused or in standby.
»
The system may be pressurized when paused.
»
Monitor the waste reservoir to prevent overflow during operation.
Start a Purification
1. Ensure that a sufficient quantity of the correct solvent
is present in each solvent reservoir and that the waste
reservoir has sufficient capacity for the run. To view the
estimated solvent consumption for the run, press
Solvents
and Waste .
2. Start the purification by pressing . If the button is
disabled, press in the bottom right corner for more
information.
If solvent and waste monitoring is enabled, you will be
notified if there is not enough solvent or waste capacity
for the run when you press .
3. When the
Sample Load
dialog opens, load your sample.
4. Start the gradient run by pressing the appropriate button in
the Sample Load dialog. We recommend that a gradient run
is not started until the UV lamp is sufficiently warmed up.
Figure 25. The default run view with the Spektra software license
installed.
Monitor the Purification in Progress
While a purification is running, a dynamic chromatogram and
the programmed gradient are displayed in the run view (see
Figure 25). If a Spektra software license has been installed on
your system, an absorbance spectrum for the whole detector
range is also displayed.
You can expand the chromatogram by pressing (full width) or
(full size), the spectrum (if available) by pressing and the
gradient table by pressing . Change the length unit by selecting
CV , mm:ss , or mL from the drop-down menu in the gradient
table (see Figure 25) or in the fully expanded chromatogram
(see Figure 26).
Figure 26. The chromatogram can be expanded to full width or full size.
Fractions
Fractions that are already collected can be located by matching
their colors and numbers in the chromatogram with the vessel
colors and numbers in the
Rack panel (see Figure 25). Fractions
from the same peak have the same color. Fractions only
collected due to the
Collect All
option are colored gray.