Biotage Selekt Enkel, Руководство пользователя

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10 Biotage
®
Selekt Enkel User Manual | © Biotage 2022
Set Up a Purification
Figure 22. The Collection panel expanded on a system with a Spektra
software license.
Specify the Rack Parameters
Load Racks
Rack type is selected by expanding the Rack panel
(see Figure 23) and selecting the correct rack in the
corresponding
Select Rack drop-down list.
Selekt racks can be automatically identified
R
by the system
when placed on the collection bed. RFID racks have the icon
to their right in the expanded
Rack panel (see Figure 31 on
page 13).
Figure 23. The Rack panel expanded on a system with extended
collection bed. Rack A was used in the previous run but still has empty
vessels that can be used by the next run. Rack C is disabled and will not
be used for collection. The Select Rack drop-down list shows the available
rack types.
Assign Racks to the Run
To assign or remove a rack from the run, expand the
Rack
panel
(see Figure 23) and set/clear the check box in front of the rack.
The following racks will automatically be assigned to your run:
»
A rack that you select in the Rack panel.
»
A rack that was assigned to but not used by the previous run.
»
The last rack used in the previous run, if it still
has one or more empty vessels or rows that are
allowed to be used in the next run according to the
system settings (see “Rack Sharing”below).
Note: If you do not want to share the last rack used in the
previous run, either remove it from the system by selecting
None
in the corresponding
Select Rack
drop-down list or clear
the check box in front of the rack.
Rack Sharing
The system offers the possibility to have the first fraction(s) of
a run collected in the last rack used by the previous run if the
following applies:
»
The Start Collection parameter in the system settings
is set to
First Empty Row
or
First Empty Vessel
.
»
The last rack used in the previous run:
»
Contains at least one row of empty vessels
or one empty vessel, depending on the
Start
Collection setting (see previous bullet).
»
Has not already been shared by five runs.
»
Is enabled in the Rack panel when the run is started.
Figure 24. There are two Start Collection options in the system settings
that allow two consecutive runs to share a rack, First Empty Row and First
Empty Vessel. The first collection vessel to be used is highlighted with
a thicker border.
Collection Order
The system uses the assigned racks in alphabetical order in
regard to their positions on the tray. The dispensing order is
left to right starting with the front vessel row. The first vessel
that will be used for the run is highlighted with a thicker border;
see Figure 24.
Note: If the run is sharing a rack with the previous run,
collection will start in the shared rack independent of the
positions of other racks assigned to the run. For more
information see “Rack Sharing” above.
Max Fraction Volume
If you want to use a lower fraction volume than the one defined
for the selected rack(s), expand the
Rack panel, enable the
Max Fraction Volume option (see Figure 23), and enter the
desired volume. Note that this setting will only be applied on
racks with a higher vessel volume.