Biotage Selekt Enkel, Руководство пользователя

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5
Prepare the System
Mount Column Holders
The system is shipped with column holders for Sfär columns
of different sizes. Ensure that they are mounted in the desired
positions on the system.
Figure 11 shows how to mount a column holder on the right side
of the system. There are also two positions for column holders
on the front of the system that can be used for Biotage columns
up to 50g; see Figure 12.
Figure 11. Slide the column holder screw into the desired position on
the right side of the system and then place the holder over the screw
and fasten.
Figure 12. Biotage columns up to 50g can be placed on the front of
the system.
Inspect the Tubing
Warning
»
Shut down the system before replacing any tubing. Use only
tubing designed for the Selekt Enkel system and supplied by
Biotage.
Inspect all tubes before each run to ensure that they do not
show signs of wear or damage and that they are properly
connected and tightened. Use caution when finger tightening
fittings to prevent stripped threads or crushed ferrules.
All external tubing on the system except for the tubing on the
collection arm can be replaced by the user.
Note: All tube types, dimensions, and lengths are essential
for the performance of the system. Only replace tubes with the
equivalent tubes designed for the Selekt Enkel system and
supplied by Biotage.
Prepare the System
Start Up the System
Turn on the system using the power switch located on the left
side of the system; see Figure 13.
Figure 13. The power switch is located on the left side of the system.
Assign Solvents and Set Reservoir Volumes
When a purification is run, the software determines which
solvent inlets are connected to the solvents used in the run.
Note: For reversed-phase purification with methanol and
water, it is strongly recommended to premix the water with
5% of methanol and degass either through vacuum or
sonication. Degassing of protic solvent blends decreases
out-gassing of entrapped air during gradient elution, which
will impact gradient performance and flow rates.
Note: With solvent and waste monitoring enabled (see
page 24), the capacity and current fluid level must be entered
each time you empty a waste reservoir or refill a solvent.
Note: Both solvent inlets must be primed with solvent to
achieve the specified pump performance.
1. In the software, press Menu and then Solvent Setup .
2. Assign a solvent to each solvent inlet using the solvent
drop-down lists (see Figure 14).
To add solvents to the list, press
Solvent Administration
.
For more information, see page 22.
3. If solvent monitoring is enabled, enter the capacity and
the current solvent level of each solvent reservoir.
4. If waste monitoring is enabled, enter the capacity and
the current waste level of the waste reservoir.
5. Prime the solvent inlets that have been assigned a new
solvent; see below.