10
Biotage
®
Flash 75/150 User Manual | © Biotage 2018
Installation
Set Up the Solvent Reservoir
Warning
»
The outlet of the safety relief valve must be directed away from
the operator.
»
When closing the solvent reservoir lid, ensure that it is seated
properly to ensure a secure seal.
1. Remove the solvent reservoir (see C in Figure 13 on
page 11) from the shipping container.
2. If the two-way on/off valve is not installed:
a. Remove the on/off valve from the inside of the reservoir.
b. Install the on/off valve into the 1/4" NPT fitting on the
solvent reservoir. Turn the valve clockwise by hand.
Use a wrench (not provided) on the hex section of the
adapter to fully tighten it into the solvent reservoir.
c. Install the lid on the solvent reservoir by tilting and
lowering it into the mouth of the reservoir. Hand-tighten
the clamp to secure the lid and ensure that the lid is
seated properly to ensure a secure seal.
3. Place the solvent reservoir in a convenient spot on the
benchtop or floor of the fume hood. Ensure that you leave
enough space for your fraction collection vessels and
that the outlet of the safety relief valve (see Figure 5 on
page 4) is directed away from the operator.
Attach the Inert Gas Supply
and Solvent Lines
Warning
»
Always connect inert gas such as nitrogen to the gas inlet on the
AM-190 manifold.
»
Follow all applicable safety procedures when working with
bottled gas.
»
Set the inert gas supply pressure to between 100 and 125 psi
(6.9 to 8.6 bar).
»
Ensure that the inert gas tubing connected to the system cannot
come in contact with chemicals. Corrosives and solvents can
dissolve the tubing.
»
Ensure that the tubes are not longer than necessary for your
setup so that there is no risk of stepping or tripping over them.
»
Do not over-tighten the fittings or the tubing may become
damaged.
The start-up kit includes all of the tubing necessary for
operation (see page 5).
1. Ensure that:
a. The inert gas supply is turned off.
b. The two-way valve on the solvent reservoir is closed.
c. The three valves on the AM-190 manifold are set to their
off/vent positions (see Table 2 on page 3)
2. Install a pressure regulator set to a maximum of 125 psi
(8.6 bar) to your inert gas supply.
3. Install the brass fitting supplied with the start-up kit (the
two-part assembly with a 3/8" NPT reducing bushing and
a 1/4" NPT instant-tight tubing connector):
»
If using an inert gas supply in the fume hood, remove the
standard hose barb and replace it with the brass fitting.
»
If using bottled inert gas, remove the 3/8" NPT reducing
bushing and then thread the 1/4" instant-tight tubing
connector directly onto your pressure regulator.
4. Connect all the tubing, except the fraction collection tube,
as shown in the left schematic in Figure 13 or, if not using
a SIM, as shown in Figure 12. The fraction collection tube is
supplied with the grounding kit and is connected during the
grounding of the system; see the instructions on page 12.
Cut the tubing so that they are long enough for your setup.
The system has both instant-tight fittings and fittings with
nuts and ferrules, see the instructions below. Test the seal
by pulling on the tubing.
Note:
All tubing should be cut with square ends using a tube
cutter. Do not use scissors as they tend to flatten the tubing
and leave a rough edge.
Note:
If using a SIM, the clear PTFE tubing attached to the
solvent reservoir must be long enough to reach both to the
radial compression module (for when equilibrating and
loading liquid samples, see the left schematic in Figure 13)
and the SIM (for when loading solid samples, see the right
schematic in Figure 13).
Note:
The Flash 75 start-up kit contains both 1/4" and 1/8"
clear PTFE tubing. The 1/8" tubing is to be used between the
SIM and the radial compression module.
Fitting with Nut and Ferrules
1. Slide the nut and ferrules onto the tube as shown in Figure 11.
2. Push the tube into the fitting until it seats and then finger-
tighten the nut. Use a 7/16" or 9/16" wrench to turn the nut
1 to 1¼ turn past finger-tight (the first time, after that less is
required). Do not over-tighten.
3. Test the seal by gently pulling on the tube.
Figure 11. How to slide the nut and ferrules onto the tubing.
Содержание Flash 150
Страница 1: ...Biotage Flash 75 150 User Manual ...
Страница 34: ...32 Biotage Flash 75 150 User Manual Biotage 2018 Notes Notes ...
Страница 35: ......