14
Biotage
®
Flash 75/150 User Manual | © Biotage 2018
How to Use the System
Warning
»
Before operating the system, please read and observe the
safety requirements in the “Safety” section on page 7.
Note:
We recommend that you keep a logbook of all the runs
performed on the system (with the date, time, user, used
solvent or solvent mixture, and pressure settings for each run)
to keep track of the pressurized cycles.
Fill the Solvent Reservoir
1. Ensure that the two-way valve on the top of the solvent
reservoir is closed; see Figure 17.
2. Ensure that the inert gas supply is turned off and that the
three valves on the AM-190 manifold are set to their off/vent
positions (see Table 2 on page 3).
3. Take the necessary precautions to avoid exposure to harmful
gases and open the lid by loosening the clamp screw and
then tilting the lid.
4. Ensure that the system is grounded; see “Ground the
System” on page 12.
5. Fill the solvent reservoir with the desired solvent.
Note:
If the solvent reservoir is not empty, ensure that you
fill it with the same solvent or solvent mixture.
6. Put the lid back in place by tilting and lowering it into the
mouth of the solvent reservoir. Hand-tighten the clamp to
secure the lid.
Figure 17
Closed
Open
. The two-way on/off valve on the solvent reservoir open (left)
and closed (right).
Insert a Flash Cartridge
Warning
»
Never run the system without a cartridge, or with the wrong
size of cartridge.
»
Never loosen the V-band clamps when the radial compression
module is under pressure. A red pressure indicator on the radial
compression module sticks out when the pressure is above
20 psi (1.4 bar); see Figure 2 on page 2.
»
Inspect the V-band clamp before and after each use. The clamp
must be replaced when it shows any signs of wear and tear, or
at 500 pressure cycles, whichever comes first. Only use clamps
and screws supplied by Biotage.
»
Confirm that the V-band clamp is tight before compressing the
radial compression module. Only hand-tighten the clamp to a
maximum torque of 100 lbf-in (11.3 Nm). If the cartridge is
not sealed properly, replace the O-ring and retry, or contact
Biotage 1-Point Support.
1. Ensure that the three-way valve on the radial compression
module and the two-way valve on the top of the solvent
reservoir are closed.
2. Ensure that the inert gas supply is turned off and that the
three valves on the AM-190 manifold are set to their off/vent
positions (see Table 2 on page 3).
3. Remove any outer plastic wrapping from the cartridge.
You may wish to keep label information for your records.
4. Remove the end caps from the cartridge and keep them.
5. Loosen the upper and lower V-band clamps.
6. Remove the top head assembly from the radial compression
module.
7. Lower the cartridge into the radial compression module and
confirm that it is level with the top of the barrel.
Note:
If the cartridge does not go all the way into the radial
compression module, loosen the lower V-band clamp until
the cartridge top is level with the top of the barrel.
8. When the cartridge is level with the top of the barrel, ensure
that the O-ring is secure inside the groove in the top lip of the
barrel and that it is not damaged; see Figure 26 on page 19.
Note:
If the O-ring shows any signs of wear or damage,
replace it. Two spare O-rings are supplied with the system.
9. Place the top head assembly back in place and hand-tighten
the V-band clamps alternating between the upper and lower
until secure.
Tightening the V-band clamp will engage the knife-edge seal
into the cartridge and engage the O-ring to seal the inert
gas pressure within the barrel. Note that the V-band clamp
should not be over-tightened. Maximum recommended
tightening torque is 100 lbf-in (11.3 Nm).
How to Use the System
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