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16
Biotage
®
Flash 75/150 User Manual | © Biotage 2018
How to Use the System
Load Sample and Collect Fractions
A liquid sample or a solid sample that has been pre-adsorbed
on silica can be loaded onto the equilibrated cartridge using
a sample injection module (SIM); see Figure 19 and Figure 20.
Figure 19
PRR-01
BIOTAGE
®
FLASH 75/150
CHROMATOGRAPHY
AM-190 MANIFOLD
PRR-02
M
V
-01
PG-01
PG-02
M
V
-03
M
V
-02
N
2
N
2
N
2
A
B
. Liquid sample loading using a sample injection module (SIM).
Figure 20
PRR-01
BIOTAGE
®
FLASH 75/150
CHROMATOGRAPHY
AM-190 MANIFOLD
PRR-02
M
V
-01
PG-01
PG-02
M
V
-03
M
V
-02
N
2
N
2
N
2
A
B
. Solid sample loading using a sample injection module (SIM).
Alternatively, if the sample is soluble in a small volume of
solvent, it can be injected with a syringe through a Luer-lock
injection port on the radial compression module; see
Figure 21. The port is fitted by disconnecting the SIM outlet
tube from the radial compression module and replacing it with
the Luer-lock injection port (supplied with Flash 150 systems).
Figure 21. Alternatively, if the sample is soluble in a small volume of
solvent, it can be injected with a syringe through a Luer-lock injection
port on the radial compression module.
Load the Sample into the SIM
1. Ensure that the
MV-02
valve on the AM-190 manifold is set
to its off/vent position (see Table 2 on page 3) and the
two-way valve on the solvent reservoir is closed.
2. If loading a solid sample, close the three-way valve on the
radial compression module and disconnect the solvent tube
connected between the solvent reservoir and the radial
compression module from the module and connect it to the
SIM; see Figure 20.
Note:
The solvent tube contains solvent; please ensure
to take the necessary precautions to avoid spillage when
disconnecting the tube.
3. Insert a bottom frit into the SIM's frit holder:
a. Push one bottom frit into a sealing ring.
b. Unscrew the lower half of the frit holder and insert the
sealing ring and frit into the lower half; see Figure 22.
Note:
Ensure that the plastic frit is at the bottom of the
frit holder.
c. Screw the lower half of the frit holder back in place.
Finger-tight is normally adequate.
Figure 22. Inserting a bottom frit into the lower half of the frit holder.
4. Loosen the clamp on the SIM and remove the top assembly
(clamp, lid with the three-way valve, and sanitary gasket).
5. Insert a top frit of the correct diameter into the SIM and push
it down to the bottom using the frit insertion tool included in
the SIM kit; see Figure 23.
Figure 23. Inserting a frit into the SIM 2000 body using its frit insertion
tool. Note that the insertion tool for SIM 500 and SIM 1000 does not have
a handle.
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