11
Decontamination:
For complete decontamination of the lower parts place the tip
ejector collar (1), tip cone holder (2), tip cone (3) and tip cone
cylinder (4) (only the models
m
5000 and
m
10 000) into a beaker
containing Biohit Proline‚ Biocontrol and leave for at least 30
minutes. Wipe the piston with Biohit Proline‚ Biocontrol and the
lint-free cloth. Rinse the parts with distilled or sterile water. Let the
parts dry. Grease the piston and seal according to the instructions
given earlier in this chapter.
Reassembling:
1. Pipettes
m
3,
m
10,
m
20,
m
100,
m
200,
m
1000:
Carefully place the tip cone (3) on the piston and attach it by
screwing the tip cone holder (2) clockwise.
Pipettes
m
5000 and
m
10 000:
Carefully place the tip cone cylinder (4) on the piston and screw
clockwise. Make sure that the tip cone (cylinder) is properly
tightened. Avoid over tightening.
2. Attach the tip ejector collar (1) by screwing it clockwise.
3. Place the new tip cone
fi
lter on its place.
4. Press the operating button several times to ensure that the
grease has spread evenly.
5. Check the performance of the pipette.
Note
: It is always necessary to check the performance of the
pipette after in-house service or maintenance.
11.3. Sterilization of the pipette
The entire
m
LINE pipette can be
sterilized by steam autoclaving
at 121°C, (252°F), 1 atm for
20 minutes. Remove the tip
cone filter (if fitted, Chapter
8.3). The single channel
pipettes can be autoclaved
without special preparations.
Unscrew the lower part of
the multichannel pipettes by
holding the connecting collar and turning the lower part 360°
counterclockwise (Fig. 10).
Put the pipette into the sterilization bag
and place it into the autoclave. After autoclaving the pipette must be
cooled down and left to dry overnight before use. Screw the lower
part of the multichannel pipettes by holding the connecting collar and
turning the lower part 360° clockwise. Make sure that the lower part
is properly tightened. It is recommended to check the performance of
the pipette after every autoclaving. It is also recommended to grease
the piston/seal of the pipette after every 10 autoclaving.
360°
Fig. 10.
Connecting
collar
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