9
9.3. Repetitive reverse technique
1. Follow the reverse technique steps 1 to 5.
2. Continue pipetting by repeating steps 3 to 5 as long as
needed.
3. Finally discard the remaining liquid by pressing the operating
button to the second stop.
10. RECOMMENDATIONS FOR GOOD PIPETTING
• Make sure that the tip is
fi
rmly attached to the tip cone.
• Hold the pipettor vertically when aspirating the liquid and place
the tip only a few millimeters into the liquid.
• Always control the operating button slowly and smoothly.
• Pre-rinse the tip before aspirating the liquid by
fi
lling and
emptying the tip for three to
fi
ve times. This is important
especially when pipetting liquids with a viscosity and density
greater than water or liquids with high vapor pressure (e.g.
ethanol).
• Check that the pipette, tip and liquid are at the same
temperature.
• When pipetting liquids with temperatures different to the
ambient temperature change the tip after each pipetting. Do
not pre-rinse the tip.
• To avoid contamination, do not rest the pipette on its side
especially with the tip attached.
• Change the tip cone
fi
lter regularly (recommendation after 50
-250 pipetting cycles).
• Never strike the tip cone against the tip tray when mounting the
tip as this can damage the pipette.
• Avoid rough handling and do not drop the pipette.
• Avoid exposing the unit to extreme temperature changes,
humidity and dust (operating temperature from 15°C to 40°C).
11. MAINTENANCE
Biohit
m
LINE pipettes have been designed for easy in-house
service. If the pipette is used daily it is recommended to clean/
decontaminate and check the performance of the pipette every
three months. Biohit also provides complete repair and recalibration
service for your pipette including a service report and performance
certi
fi
cate.
Note
: Please make sure that the pipette has been decontaminated
before you send it to us or your local representative. Please advise
if any hazardous material has been used with your pipette.
Note
: The use of the tip cone
fi
lters may prolong the interval of the
service. Change the
fi
lters regularly.
Note
: It is recommended to always use gloves when cleaning the
pipette.
Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com
