background image

13

13. For conformity evaluation calculate the random error of the 

measurement:

 

 

 

 

          

as standard deviation s =   

(V

i

 - 

v

̄

 )

2

     n = number of    

 

 

 

 

            measurement (10)

                                                      n - 1 

 

or as coef

fi

 cient of variation CV = 100

s

/

v

̄

 

14.  Compare the systematic error (inaccuracy) and random error 

(imprecision) with the values in the performance speci

fi

 cations 

- (p. 49) or the speci

fi

 cations of your own laboratory. 

 

If the results fall within the speci

fi

 cations, the pipette is ready 

for use. Otherwise check both systematic and random errors 

and, when necessary, proceed to the recalibration procedure 

(Chapter 12.2).

Note

: Systematic error (inaccuracy) is the difference between the 

dispensed volume and the selected test volume. Random error 

(imprecision) is the scatter of the dispensed volumes around the 

mean of the dispensed volume. (ISO 8655-1.)

Note

: Biohit speci

fi

 cations are achieved in strictly controlled condi-

tions (ISO 8655-6). The user should establish own speci

fi

 cations 

based on the 

fi

 eld of use and the accuracy requirements placed on 

the pipettor (ISO8655-1). 

Table 1

Note:

 This method is based on ISO 8655.

  

  Z-values 

(

μ

l/mg):

Temp. (°C) 

 

Air Pressure (kPa)

  

95 

100 

101.3 

105

 20.0 

1.0028 

1.0028 

1.0029 

1.0029 

 20.5 

1.0029 

1.0029 

1.0030 

1.0030

 21.0 

1.0030 

1.0031 

1.0031 

1.0031

 21.5 

1.0031 

1.0032 

1.0032 

1.0032

 22.0 

1.0032 

1.0033 

1.0033 

1.0033

 22.5 

1.0033 

1.0034 

1.0034 

1.0034

 23.0 

1.0034 

1.0035 

1.0035 

1.0036

 23.5 

1.0036 

1.0036 

1.0036 

1.0037

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Содержание mLINE

Страница 1: ...t Service Center Repairs Experienced Engineers and Technicians on staff in our State of the art Full Service In House Service Center Facility InstraView Remote Inspection Remotely inspect equipment be...

Страница 2: ...mLINE Instruction Manual Bedienungsanleitung Mode d emploi Manual Usuario Instruzioni d impiego Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Страница 3: ...seal 7 Calibration nut cover 8 Display 9 Handle 10 Tip ejector 11 ID window 12 ID tag 13 Handle collar 14 Cap set of 5 colours Ersatzteile Spitzenabwurf Konusfilterhalter Spitzenkonus Konuszylinder K...

Страница 4: ...satzteile Spitzenkonus geh use Spitzenabwurfs balken Spitzenkonus Kolben Kolbenzu sammensetzung Abdeckung Kalibrierschraube Display Handgriff Spitzenabwerfer ID Fenster ID Aufkleber Griffhalter Abdeck...

Страница 5: ...anual 1 Bedienungsanleitung 17 Mode d emploi 33 Manual Usuario 49 Instruzioni d impiego 65 81 Specifications 97 mLINE Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan sc...

Страница 6: ...UES 7 9 1 Forward pipetting Fig 5 8 9 2 Reverse pipetting Fig 9 8 9 3 Repetitive reverse technique 9 10 RECOMMENDATIONS FOR GOOD PIPETTING 9 11 MAINTENANCE 9 11 1 Daily cleaning of the outer surface o...

Страница 7: ...pipetting can cause RSI The manufacturer is not responsible for RSI or any related diseases caused by prolonged pipetting Safe Cone filters Colour Volume 50 pcs bag Cat No Code Channels Range Incremen...

Страница 8: ...ck Colour coding Tip ejector collar Tip cone ID window and tag Finger support Handle Calibration nut Safe Cone filter Tip cone Tip cone housing Tip ejector bar Safe Cone filter Artisan Scientific Qual...

Страница 9: ...alibration tool also tube and ID window opener 5 Pipette holder 6 Identification tags 7 Safe Cone filters pipettors 10 l 8 Grease 9 Instruction manual 10 Performance certification in accordance with I...

Страница 10: ...stall the holder by pressing it against the edge of the shelf Fig 2 4 Place the pipette onto the holder 6 2 Carousel stand A convenient and ergonomical Carousel Stand Fig 3 for six Biohit mLINE pipett...

Страница 11: ...lume range of the pipette Chapter 1 8 2 Sealing and ejecting tips To ensure the maximum accuracy and precision Biohit tips are recommended to be used with mLINE pipettes Before fitting a tip ensure th...

Страница 12: ...lter ejector without touching the filters by hand Remove the operating button cap and eject the filter by pressing the operating button down Fig 7 Make sure that the filter is disposed into a suitable...

Страница 13: ...p Fig 8 0 1 2 9 2 Reverse pipetting Fig 9 1 Fit the tip onto the pipette tip cone 2 Press the operating button all the way to the second stop 3 Place the tip just under the surface of the liquid 2 3 m...

Страница 14: ...tte on its side especially with the tip attached Change the tip cone filter regularly recommendation after 50 250 pipetting cycles Never strike the tip cone against the tip tray when mounting the tip...

Страница 15: ...the tip ejector collar 1 counterclockwise and remove it 3 Pipettes m3 m10 m20 m100 m200 m1000 Unscrew the tip cone holder 2 counterclockwise and carefully remove it with the tip cone 3 Pipettes m5000...

Страница 16: ...ase has spread evenly 5 Check the performance of the pipette Note It is always necessary to check the performance of the pipette after in house service or maintenance 11 3 Sterilization of the pipette...

Страница 17: ...fit the tip onto the tip cone 3 Fill the tip with test water and expel to waste five times to reach a humidity equilibrium in the dead air volume 4 Replace the tip Pre wet the tip by filling it once...

Страница 18: ...e and the selected test volume Random error imprecision is the scatter of the dispensed volumes around the mean of the dispensed volume ISO 8655 1 Note Biohit specifications are achieved in strictly c...

Страница 19: ...d of the calibration nut located at the backside of the handle with the aid of the calibration tool Fig 11 2 Place the hexagonal head of the calibration tool into the hole of the calibration nut 3 Tur...

Страница 20: ...r servicing Pipette out of Incorrect operation Follow instructions established specifications Unsuitable tip Use original tips Calibration altered Recalibrate Operating button Liquid has penetrated Cl...

Страница 21: ...OUTSIDE THEIR SPECIFICATIONS CONTRARY TO THE INSTRUCTIONS GIVEN IN THIS MANUAL OR WITH OTHER THAN THE MANUFACTURER S ORIGINAL TIPS Each Biohit mLINE pipette is tested before shipping by the manufactur...

Страница 22: ...l 1000 l 0 60 0 20 500 l 0 60 0 20 100 l 1 00 0 40 725080 1 500 5000 l 5000 l 0 50 0 20 2500 l 0 60 0 30 500 l 2 00 0 60 725090 1 1 10 ml 10 000 l 0 6 0 2 5000 l 1 2 0 3 1000 l 3 0 0 6 725120 8 0 5 10...

Страница 23: ...it gr ter Sorgfalt zusammengetragen Biohit ist nicht verantwortlich f r Fehler oder Auslassungen und beh lt sich das Recht vor nderungen ohne Ank ndigung vorzunehmen Alle Marken sind Eigentum von Bioh...

Страница 24: ...t Service Center Repairs Experienced Engineers and Technicians on staff in our State of the art Full Service In House Service Center Facility InstraView Remote Inspection Remotely inspect equipment be...

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